B-ALL患儿VDLP化疗缓解后髓源抑制性细胞的水平变化及其与免疫系统的关系
发布时间：2018-07-20 11:53
【摘要】：目的:观察并研究外周血髓系来源的抑制性细胞在B系急性淋巴细胞白血病(B-ALL)患儿经长春地辛、柔红霉素、培门冬酶和强的松(Vindesine、Daunorubicin、Pegaspargase、Prednisone,VDLP)方案化疗缓解后的变化并分析其与免疫系统的可能关系。方法:选取我院2015年8月至2016年8月期间收治的30例经VDLP方案化疗后缓解的B-ALL患儿为B-ALL组,选取30例正常健康儿童为对照组,分别抽取2组的外周血,采用流式细胞术检测并计算其外周血CD33~+细胞和CD33~+HLA-DR~-、CD14~+CD33~+HLA-DR~-、CD15~+CD33~+HLA-DR~-骨髓源抑制性细胞的比例,分析其在B-ALL患儿经VDLP方案化疗缓解后的变化及其与免疫系统的关系。结果:治疗后B-ALL组和对照组外周血CD33~+细胞比例无明显差异(P0.05);治疗后B-ALL组外周血患儿CD33~+细胞比例显著高于治疗前(P0.05)。治疗后B-ALL组患儿外周血CD33~+HLA-DR~-、CD14~+CD33~+HLA-DR~-、CD15~+CD33~+HLA-DR~-髓系来源抑制性细胞比例均显著低于对照组健康儿童(P=0.02,P=0.02,P=0.04)。治疗后B-ALL组患儿外周血CD33~+HLA-DR~-、CD14~+CD33~+HLA-DR~-、CD15~+CD33~+HLA-DR~-骨髓源抑制性细胞比例均高于治疗前,但差异均无统计学意义(均P0.05)。结论:外周血骨髓源的抑制性细胞在B-ALL患儿行VDLP方案化疗后完全缓解期比例高于治疗前,显著低于正常值,这可能与化疗后患儿免疫系统还未完全恢复有关。
[Abstract]:Objective: To observe and study the changes of the suppressor cells derived from peripheral blood myeloid in B system acute lymphoblastic leukemia (B-ALL) children with Changchun digoxin, daunorubicin, aspartate and Jodison (Vindesine, Daunorubicin, Pegaspargase, Prednisone, VDLP) and to analyze the possible relationship with the immune system. In our hospital from August 2015 to August 2016, 30 children with B-ALL after VDLP chemotherapy were treated as B-ALL group. 30 normal healthy children were selected as the control group. 2 groups of peripheral blood were extracted respectively. Flow cytometry was used to detect and calculate the peripheral blood CD33~+ cells and CD33~+ HLA-DR~-, CD14~+CD33~+HLA-DR~-, CD15~+CD33~+HLA-DR~-. The ratio of bone marrow suppressor cells was analyzed, and the relationship between the changes in the VDLP regimen and the immune system in children with B-ALL was analyzed. Results: there was no significant difference in the proportion of CD33~+ cells in the peripheral blood of the B-ALL group and the control group after treatment (P0.05), and the proportion of CD33~+ cells in the peripheral blood of the B-ALL group was significantly higher than that before the treatment (P0.05). The percentage of peripheral blood CD33~+HLA-DR~-, CD14~+CD33~+HLA-DR~-, and CD15~+CD33~+HLA-DR~- in the B-ALL group was significantly lower than that in the control group (P=0.02, P=0.02, P=0.04). The percentage of the peripheral blood CD33~+HLA-DR~-, CD14~+CD33~+HLA-DR~-, and CD15~+CD33~+HLA-DR~- myelogenous cells in the peripheral blood of the group B-ALL was higher than that of the group B-ALL. Before treatment, the difference was not statistically significant (all P0.05). Conclusion: the proportion of peripheral blood bone marrow suppressor cells in B-ALL children's complete remission stage after VDLP regimen was higher than that before the treatment, significantly lower than the normal value, which may be related to the incomplete recovery of the immune system after chemotherapy.
【作者单位】： 南京医科大学附属儿童医院血液肿瘤科;南京大学附属鼓楼医院血液科;
【分类号】：R733.71
本文编号：2133431
[Abstract]:Objective: To observe and study the changes of the suppressor cells derived from peripheral blood myeloid in B system acute lymphoblastic leukemia (B-ALL) children with Changchun digoxin, daunorubicin, aspartate and Jodison (Vindesine, Daunorubicin, Pegaspargase, Prednisone, VDLP) and to analyze the possible relationship with the immune system. In our hospital from August 2015 to August 2016, 30 children with B-ALL after VDLP chemotherapy were treated as B-ALL group. 30 normal healthy children were selected as the control group. 2 groups of peripheral blood were extracted respectively. Flow cytometry was used to detect and calculate the peripheral blood CD33~+ cells and CD33~+ HLA-DR~-, CD14~+CD33~+HLA-DR~-, CD15~+CD33~+HLA-DR~-. The ratio of bone marrow suppressor cells was analyzed, and the relationship between the changes in the VDLP regimen and the immune system in children with B-ALL was analyzed. Results: there was no significant difference in the proportion of CD33~+ cells in the peripheral blood of the B-ALL group and the control group after treatment (P0.05), and the proportion of CD33~+ cells in the peripheral blood of the B-ALL group was significantly higher than that before the treatment (P0.05). The percentage of peripheral blood CD33~+HLA-DR~-, CD14~+CD33~+HLA-DR~-, and CD15~+CD33~+HLA-DR~- in the B-ALL group was significantly lower than that in the control group (P=0.02, P=0.02, P=0.04). The percentage of the peripheral blood CD33~+HLA-DR~-, CD14~+CD33~+HLA-DR~-, and CD15~+CD33~+HLA-DR~- myelogenous cells in the peripheral blood of the group B-ALL was higher than that of the group B-ALL. Before treatment, the difference was not statistically significant (all P0.05). Conclusion: the proportion of peripheral blood bone marrow suppressor cells in B-ALL children's complete remission stage after VDLP regimen was higher than that before the treatment, significantly lower than the normal value, which may be related to the incomplete recovery of the immune system after chemotherapy.
【作者单位】： 南京医科大学附属儿童医院血液肿瘤科;南京大学附属鼓楼医院血液科;
【分类号】：R733.71
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本文编号：2133431
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