阿司匹林对血小板诱导的乳腺癌MCF-7细胞上皮间质转化和迁移侵袭能力的影响

发布时间：2018-08-29 12:45

【摘要】：目的探讨阿司匹林对血小板诱导人乳腺癌MCF-7细胞(MCF-7 human breast cancer cells)上皮间质转化和迁移侵袭能力的影响。方法以未经干预的MCF-7细胞为对照组,分别用不同浓度(0.5、2.0 mmol/L)的阿司匹林、花生四烯酸激活的血小板及不同浓度阿司匹林预处理花生四烯酸激活的血小板分别干预MCF-7细胞,采用细胞划痕及Transwell实验检测肿瘤细胞迁移侵袭能力的变化。Western Blot检测肿瘤细胞上皮间质转化标记蛋白E-cadherin及Vimentin的表达。结果活化的血小板处理后与对照组相比MCF-7细胞迁移侵袭能力显著增强(P0.05),Western Blot显示E-cadherin表达显著减少(P0.01),Vimentin的表达显著增加(P0.01)。阿司匹林预处理血小板活化后干预MCF-7细胞,其迁移侵袭能力较活化血小板组显著降低(P0.05),E-cadherin表达显著增高(P0.01),Vimentin的表达显著减少(P0.05)。阿司匹林单独处理后的MCF-7细胞迁移及侵袭能力较对照组无显著变化(P0.05),E-cadherin及Vimentin的表达亦无显著改变(P0.05)。结论体外实验结果表明,阿司匹林可通过抗血小板活化,抑制血小板诱导的乳腺癌MCF-7细胞上皮间质转化和迁移侵袭能力。
[Abstract]:Objective to investigate the effects of aspirin on the translocation and invasion of human breast cancer MCF-7 cells (MCF-7 human breast cancer cells) induced by platelets. Methods the untreated MCF-7 cells were treated with different concentrations of aspirin (0.5 mmol/L), arachidonic acid-activated platelets and different concentrations of aspirin pretreated with arachidonic acid-activated platelets, respectively. Cell scratch and Transwell assay were used to detect the change of migration and invasion ability of tumor cells. Western Blot was used to detect the expression of E-cadherin and Vimentin. Results compared with the control group, the migration and invasion ability of MCF-7 cells was significantly increased (P0.05). Western Blot showed that the expression of E-cadherin decreased significantly (P0.01) and the expression of Vimentin increased significantly (P0.01). After pretreatment of platelet activation with aspirin, the migration and invasion ability of MCF-7 cells was significantly lower than that of activated platelet group (P0.05), the expression of E-cadherin was significantly increased (P0.01) and the expression of Vimentin was significantly decreased (P0.05). The expression of E-cadherin and Vimentin in MCF-7 cells treated with aspirin alone had no significant change compared with the control group (P0.05). Conclusion Aspirin can inhibit platelet-induced epithelial mesenchymal transformation and migration and invasion of breast cancer MCF-7 cells by anti-platelet activation in vitro.
【作者单位】：南方医科大学第三附属医院普外科;
【基金】：广州市天河区科技计划项目(201504KW038)
【分类号】：R737.9

【相似文献】