5-氟尿嘧啶耐药的晚期胃癌患者血清外泌体miRNA表达谱分析

发布时间：2018-09-11 08:17

【摘要】：目的 :探讨对5-氟尿嘧啶(5-uorouracil,5-Fu)原发性耐药和继发性耐药的晚期胃癌患者血清外泌体mi RNA的表达差异。方法:收集6例以5-Fu为基础化疗的原发性和继发性耐药的晚期胃癌患者化疗前及耐药后的血清样本,提取外泌体miRNA,通过高通量测序技术筛选显著差异表达的miRNA,应用生物信息学技术预测差异表达miRNA的靶基因,并进行GO(gene ontology)分析及KEGG(Kyoto Encyclopedia of Genes and Genomes)通路分析。结果:原发性耐药晚期胃癌患者血清外泌体中有108个miRNA明显上调(P值均0.01),137个miRNA明显下调(P值均0.01);继发性耐药患者耐药前后有2个miRNA表达有差异(P值均0.05)。差异表达的miRNA靶基因主要与细胞周期、磷酸化和细胞连接形成等多种生物学过程及磷脂酰肌醇3激酶(phosphoinositide 3-kinase,PI3K)/蛋白激酶B(protein kinase B,PKB,又称Akt)和丝裂原活化蛋白激酶(mitogen-activated protein kinase,MAPK)等多条通路有关。结论:初步筛选出对5-Fu原发性和继发性耐药胃癌患者及继发性耐药患者耐药前后差异表达的血清外泌体miRNA,这些差异表达的miRNA与多种生物学过程及通路有关。
[Abstract]:Objective: to investigate the difference of serum exocrine mi RNA expression in patients with 5 fluorouraciline (5 fluorouracil) primary and secondary resistant advanced gastric cancer. Methods: the serum samples of 6 patients with primary and secondary drug-resistant advanced gastric cancer before and after chemotherapy based on 5-Fu were collected. Exocrine miRNA, was extracted and screened by high-throughput sequencing. Bioinformatics was used to predict the target genes of differentially expressed miRNA. GO (gene ontology) analysis and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway analysis were performed. Results: 108 miRNA were up-regulated and 137 miRNA were down-regulated in patients with primary drug-resistant advanced gastric cancer (P0.01), and there were two miRNA expressions in secondary drug-resistant patients before and after drug resistance (P0. 05). The differentially expressed miRNA target genes are mainly related to many biological processes, such as cell cycle, phosphorylation and cell junction formation, and several pathways, such as phosphoinositide 3-kinase PI3K / protein kinase B (protein kinase PKB, and mitogen-activated protein kinase (mitogen-activated protein kinase,MAPK). Conclusion: the differential expression of serum exocrine miRNA, in patients with primary and secondary drug-resistant gastric cancer and secondary drug-resistant patients with 5-Fu were preliminarily screened. The differential expression of miRNA was related to a variety of biological processes and pathways.
【作者单位】：上海市浦东新区浦南医院肿瘤科;上海交通大学附属第一人民医院肿瘤科;上海交通大学医学院附属仁济医院肿瘤科;
【基金】：上海市卫生和计划生育委员会科研课题计划(编号:201540303) 上海市浦东新区卫生系统学科带头人培养(编号:PWRd2014-03)~~
【分类号】：R735.2

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