外周血癌细胞中多种肿瘤标记物基因检测预测乳腺癌患者转移和预后的意义

发布时间：2018-09-11 08:49

【摘要】：目的:筛选一组可检测乳腺癌循环癌细胞的标记物基因,分析乳腺癌患者相对循环癌细胞数(Lc)与临床病理特征的关联。方法:收集乳腺癌患者142例(乳腺癌组)和正常女性60人(正常对照组)外周血标本。利用CGAP提供的SAGE Genie数据库中数字基因表达演示工具,筛选一组可用于检测乳腺癌循环癌细胞的标记物基因(FAM83A、NPY1R和KRT19)。应用定量巢式PCR方法检测研究对象外周血中标记物基因的表达水平,并通过公式计算患者Lc值。结果:在乳腺癌组患者外周血中肿瘤标记物基因FAM83A、NPY1R和KRT19的表达水平均明显高于正常对照组(P0.001)。142例乳腺癌患者中有113例(79.6%)至少表达1个肿瘤标记物基因。乳腺癌患者Lc值与其临床分期和远处转移有关联(P0.001)。Kaplam-Meier生存曲线,Lc值小于2的患者生存时间明显长于Lc值大于2者(P=0.005)。结论:筛选了一组检测乳腺癌外周血循环癌细胞的标记物基因,联合该组基因计算的Lc值与患者临床分期和远处转移有关联,并可辅助判断乳腺癌预后。
[Abstract]:Aim: to screen a group of marker genes for detecting circulating breast cancer cells and to analyze the correlation between the relative number of circulating cancer cells and clinicopathological features in breast cancer patients. Methods: peripheral blood samples were collected from 142 breast cancer patients (breast cancer group) and 60 normal women (normal control group). A set of marker genes (FAM83A,NPY1R and KRT19) which can be used to detect circulating breast cancer cells were screened by using the digital gene expression demonstration tool in SAGE Genie database provided by CGAP. The expression level of marker gene in peripheral blood was detected by quantitative nested PCR, and the Lc value of patients was calculated by formula. Results: the expression of tumor marker gene FAM83A,NPY1R and KRT19 in peripheral blood of breast cancer patients was significantly higher than that of normal control group (P 0.001). 113 cases (79.6%) of breast cancer patients expressed at least one tumor marker gene. The survival time of breast cancer patients whose Lc value was less than 2 was significantly longer than that of patients with Lc greater than 2 (P0. 001). Kaplam-Meier survival curve was less than 2 (P0. 005). Conclusion: a group of marker genes for detection of circulating cancer cells in peripheral blood of breast cancer were screened. The Lc values calculated by the combination of these genes were associated with clinical stage and distant metastasis of breast cancer patients, and could help to judge the prognosis of breast cancer.
【作者单位】：承德医学院临床技能培训中心;承德医学院基础医学院免疫学教研室;河北省承德市妇幼保健院妇幼产房;承德医学院附属医院肿瘤科;承德医学院基础医学院;
【基金】：河北省科技厅自然科学基金资助课题(H2015406014) 河北省教育厅高等学校科学技术研究项目资助课题(QN20131010) 河北省人力资源和社会保障厅人才工程培养经费资助课题(A2016002085)
【分类号】：R737.9

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