LKB1在肝细胞肝癌上皮—间质转化中的作用研究
发布时间：2018-09-11 19:38
【摘要】：目的研究LKB1在肝细胞肝癌(hepatocellular carcinoma,HCC)上皮-间质转化(epithelial-mesenchymal transition,EMT)中的作用。研究方法收集郑州大学人民医院病例资料完整的HCC标本90例、慢性乙肝肝硬化组织和肝血管瘤组织各30例。通过免疫组化的方法分别检测HCC、乙肝肝硬化组织和肝血管瘤组织中LKB1和EMT相关分子E-cadherin、N-cadherin的表达。结果选取的90例HCC标本中,LKB1阳性表达率为31.1%(28/90)。明显低于其在乙肝肝硬化组织和肝血管瘤组织中的的表达,阳性表达率分别为93.3%(28/30)和96.6%(29/30)。E-cadherin在乙肝肝硬化组织和肝血管瘤组织中的阳性表达率分别为73.3%(22/30)和66.6%(20/30),而HCC组织其阳性表达率为45.6%(41/90)。与之相反,N-cadherin在HCC组织中表现为表达增高(70.0%,63/90),乙肝肝硬化组织和肝血管瘤组织中表现为低表达(8/30,26.6%;4/30,13.3%)。LKB1在HCC中的表达与肿瘤直径、肿瘤有无包膜、门静脉受侵犯以及TNM分期有关(p0.05),而与年龄、性别、感染乙肝病毒无关;肿瘤直径、HCC包膜缺失、门静脉侵犯与E-cadherin表达降低有关(p0.05)。肿瘤包膜、TNM分期、门静脉侵犯与N-cadherin表达增高有关(p0.05)。LKB1在高分化和中分化HCC中的阳性表达率为(36.99%,27/73)高于其在低分化HCC中的阳性表达率(5.88%,1/17),存在统计学差异(p0.05)。LKB1在HCC中的表达与E-cadherin的表达呈正相关,具有统计学差异(p0.05),LKB1在HCC中的表达与N-cadherin的表达呈负相关,具有统计学差异(p0.05)。结论1.随着HCC分化程度越低,LKB1的表达也随之降低,HCC中LKB1的表达与分化程度相关;2.LKB1、E-cadherin在HCC中表达降低,N-cadherin在HCC中表达增高,LKB1表达降低与HCC上皮-间质转化相关。
[Abstract]:Objective to study the role of LKB1 in epithelial-interstitial transformation (epithelial-mesenchymal transition,EMT) of hepatocellular carcinoma (hepatocellular carcinoma,HCC). Methods 90 HCC specimens were collected from Renmin Hospital of Zhengzhou University, 30 cases from liver cirrhosis and 30 cases from hemangioma of liver. The expression of LKB1 and EMT related molecules E-cadherin in liver cirrhosis and hemangioma tissues of HCC, hepatitis B were detected by immunohistochemical method. Results the positive rate of LKB1 in 90 HCC samples was 31. 1% (28 / 90). The positive expression rates of E-cadherin in liver cirrhosis and hemangioma were 93.3% (28 / 30) and 96.6% (29 / 30), respectively. The positive expression rates of E-cadherin in liver cirrhosis and hepatic hemangioma were 73.3% (2230 / 30) and 66.6% (2030), respectively. The positive rate of E-cadherin in HCC was 45.6% (41 / 90). In contrast, the expression of N-cadherin in HCC was increased (70.063 / 90), and the expression of LKB1 in liver cirrhosis and hemangioma was low (83026.6 / 4 / 3013.3%). The expression of LKB1 in HCC was correlated with the diameter of tumor, and the tumor had no capsule. Portal vein invasion and TNM staging were correlated with age, sex and hepatitis B virus infection (p0.05). The tumor diameter of HCC was absent and portal vein invasion was associated with decreased expression of E-cadherin (p0.05). TNM staging of tumor capsule The positive expression rate of LKB1 in well-differentiated and moderately differentiated HCC was higher than that in poorly differentiated HCC (p0.05). The expression of LKB1 in HCC was significantly higher than that in poorly differentiated HCC (5.8820 / 17). There was a positive correlation between the expression of LKB1 in HCC and the expression of E-cadherin. The expression of LKB1 in HCC was negatively correlated with the expression of N-cadherin (p0.05). Conclusion 1. With the lower the differentiation of HCC, the lower the expression of LKB1, the lower the expression of LKB1E-cadherin in HCC and the lower the expression of N-cadherin in HCC. The decrease of LKB1 expression was related to the epithelial-interstitial transformation of HCC.
【学位授予单位】：郑州大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R735.7
[Abstract]:Objective to study the role of LKB1 in epithelial-interstitial transformation (epithelial-mesenchymal transition,EMT) of hepatocellular carcinoma (hepatocellular carcinoma,HCC). Methods 90 HCC specimens were collected from Renmin Hospital of Zhengzhou University, 30 cases from liver cirrhosis and 30 cases from hemangioma of liver. The expression of LKB1 and EMT related molecules E-cadherin in liver cirrhosis and hemangioma tissues of HCC, hepatitis B were detected by immunohistochemical method. Results the positive rate of LKB1 in 90 HCC samples was 31. 1% (28 / 90). The positive expression rates of E-cadherin in liver cirrhosis and hemangioma were 93.3% (28 / 30) and 96.6% (29 / 30), respectively. The positive expression rates of E-cadherin in liver cirrhosis and hepatic hemangioma were 73.3% (2230 / 30) and 66.6% (2030), respectively. The positive rate of E-cadherin in HCC was 45.6% (41 / 90). In contrast, the expression of N-cadherin in HCC was increased (70.063 / 90), and the expression of LKB1 in liver cirrhosis and hemangioma was low (83026.6 / 4 / 3013.3%). The expression of LKB1 in HCC was correlated with the diameter of tumor, and the tumor had no capsule. Portal vein invasion and TNM staging were correlated with age, sex and hepatitis B virus infection (p0.05). The tumor diameter of HCC was absent and portal vein invasion was associated with decreased expression of E-cadherin (p0.05). TNM staging of tumor capsule The positive expression rate of LKB1 in well-differentiated and moderately differentiated HCC was higher than that in poorly differentiated HCC (p0.05). The expression of LKB1 in HCC was significantly higher than that in poorly differentiated HCC (5.8820 / 17). There was a positive correlation between the expression of LKB1 in HCC and the expression of E-cadherin. The expression of LKB1 in HCC was negatively correlated with the expression of N-cadherin (p0.05). Conclusion 1. With the lower the differentiation of HCC, the lower the expression of LKB1, the lower the expression of LKB1E-cadherin in HCC and the lower the expression of N-cadherin in HCC. The decrease of LKB1 expression was related to the epithelial-interstitial transformation of HCC.
【学位授予单位】：郑州大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R735.7
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