miR-143调控PAN3蛋白的表达对B细胞淋巴瘤细胞侵袭和迁移的影响

发布时间：2018-09-12 20:59

【摘要】：目的:探讨miR-143调控PAN3蛋白的表达对B细胞淋巴瘤细胞侵袭和迁移的影响。方法:运用q PCR实验检测miR-143在正常骨髓组织和淋巴瘤血液组织中的表达情况;检测在不同种类的细胞株中miR-143的表达水平;q PCR检测miR-143对PAN3的表达水平的影响;双荧光素酶实验检测miR-143和PAN3之间的相互关系;划痕实验和Transwell侵袭实验检测miR-143表达对B细胞淋巴瘤E6-1细胞迁移和侵袭能力的影响。结果:和正常骨髓组织相比,miR-143在B细胞淋巴瘤中表达明显下调;选取miR-143表达水平较低的E6-1细胞株;双荧光素酶实验表明miR-143可以下调PAN3的表达水平,直接影响PAN3的表达活性;过表达miR-143后,E6-1细胞的侵袭和迁移能力明显受到抑制,抑制E6-1细胞的侵袭转移能力。结论:miR-143可以通过调控PAN3的表达,从而调节B细胞淋巴瘤细胞的迁移和侵袭行为。
[Abstract]:Objective: to investigate the effect of miR-143 on the invasion and migration of B cell lymphoma cells. Methods: Q PCR assay was used to detect the expression of miR-143 in normal bone marrow tissue and lymphoma blood tissue, and the expression level of miR-143 was detected in different cell lines. Q PCR was used to detect the effect of miR-143 on PAN3 expression. The relationship between miR-143 and PAN3 was detected by double luciferase assay, and the effect of miR-143 expression on the migration and invasion of B cell lymphoma E6-1 cells was detected by scratch test and Transwell invasion assay. Results: compared with the normal bone marrow tissue, the expression of miR-143 was down-regulated in B-cell lymphoma, and the E6-1 cell line with lower miR-143 expression level was selected. Double luciferase assay showed that miR-143 could down-regulate the expression of PAN3 and directly affect the activity of PAN3. After overexpression of miR-143, the invasion and migration of E6-1 cells were inhibited, and the invasion and metastasis of E6-1 cells were inhibited. Conclusion: miR-143 can regulate the migration and invasion of B cell lymphoma cells by regulating the expression of PAN3.
【作者单位】：新乡医学院第一附属医院血液科;
【基金】：河南省教育厅基金资助项目(No.2006320039)
【分类号】：R733.1

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