肿瘤相关巨噬细胞对肺腺癌的发展及耐药作用的研究
发布时间：2018-09-13 06:12
【摘要】：第一部分肿瘤相关巨噬细胞的诱导及鉴定目的:通过体外诱导确立人肿瘤相关巨噬细胞(TAM),并分析其表型特征。方法:一般观点认为,普通巨噬细胞为M1型巨噬细胞,TAM为M2型巨噬细胞。本部分首先运用佛波酯(PMA)诱导人单核细胞株THP-1细胞贴壁分化成巨噬细胞(PMA only型),运用流式细胞分析仪检测巨噬细胞(PMA only型)表面分子CD14的表达水平,然后运用人白细胞介素4(IL-4)或脂多糖(LPS)分别刺激巨噬细胞极化成为M2或M1型巨噬细胞;半定量RT-PCR法检测不同组巨噬细胞IL-6m RNA的表达水平,ELISA实验分析不同组巨噬细胞TNF-α蛋白的表达情况。结果:THP-1细胞被PMA诱导后,其细胞表面CD14的表达水平明显增高。和LPS诱导极化的M1型巨噬细胞相比,单独运用PMA诱导的巨噬细胞(PMA only型)和IL-4诱导的M2型巨噬细胞其IL-6m RNA的表达和TNF-α蛋白的表达水平明显降低。结论:成功诱导了巨噬细胞,其CD14高表达且偏向于M2型巨噬细胞;成功诱导建立了TAM,其特征为M2型巨噬细胞,表现为IL-6及TNF-α低表达。第二部分肿瘤相关巨噬细胞对肺腺癌细胞迁移、侵袭的影响及初步机制研究目的:探讨肿瘤相关巨噬细胞(TAM)对人肺腺癌A549细胞迁移和侵袭的影响,并初步分析其潜在的分子机制。方法:将肺腺癌A549细胞与肿瘤相关巨噬细胞(TAM)通过transwell小室进行间接共培养,然后收集其共培养液用作后续实验。用划痕愈合实验检测肿瘤相关巨噬细胞(TAM)对A549细胞迁移的影响;用transwell侵袭实验检测肿瘤相关巨噬细胞(TAM)对A549细胞侵袭的影响;用半定量RT-PCR,q RT-PCR和western blot法检测肿瘤相关巨噬细胞(TAM)对A549细胞VEGF-C,VEGF-D和VEGFR3表达的影响;用免疫组化法(IHC)检测VEGFR3在肺腺癌组织中的表达分布。在肿瘤相关巨噬细胞(TAM)微环境中,VEGFR3的抑制剂(MAZ51)抑制VEGFR3后,用划痕愈合实验和transwell侵袭实验分别检测A549细胞的迁移、侵袭能力。结果:肿瘤相关巨噬细胞(TAM)促进肺腺癌A549细胞的迁移和侵袭。肿瘤相关巨噬细胞(TAM)提高肺腺癌A549细胞VEGF-C和VEGFR3蛋白的表达。免疫组化(IHC)实验证实VEGFR3主要在人肺腺癌细胞中高表达。抑制VEGFR3后明显降低肺腺癌A549细胞的迁移和侵袭。结论:肿瘤相关巨噬细胞(TAM)通过提高A549细胞VEGFR3的表达从而促进肿瘤细胞的迁移和侵袭。第三部分肿瘤相关巨噬细胞介导的VEGFR3对肿瘤增殖的影响目的:探讨肿瘤相关巨噬细胞(TAM)介导的人肺腺癌细胞VEGFR3的表达对肺腺癌细胞增殖的影响,并探究其潜在的分子机制。方法:用MTT实验分析在不同浓度梯度的VEGFR3抑制剂(MAZ51)作用下,其共培养液中A549细胞的活力变化;流式细胞分析仪检测在MAZ51作用下,共培养A549细胞的凋亡情况;细胞集落形成实验分析MAZ51对共培养A549细胞增值的抑制作用。Western blot分析法检测在MAZ51作用下共培养A549细胞中MAPK信号通路以及P53,PTEN,BCL-2和MMP-2蛋白表达变化;半定量RT-PCR法检测ERK信号通路抑制剂U0126抑制ERK信号通路及si RNA干扰P53和PTEN的表达后,共培养A549细胞中P53和PTEN m RNA的表达变化;MTT实验分析si RNA干扰P53和PTEN的表达后,在MAZ51作用下,共培养A549细胞的活力变化。结果:MAZ51降低共培养A549细胞的增殖活力,促进其凋亡,降低其细胞集落形成数。Western blot实验证实MAZ51能够抑制p-ERK的活性,提高抑癌蛋白P53,PTEN的表达,降低抗凋亡蛋白BCL-2和促侵袭蛋白MMP-2的表达。抑制ERK信号通路后,P53和PTEN m RNA的表达明显提高。干扰P53和PTEN的表达后,提高了共培养A549细胞的增殖活力。结论:抑制VEGFR3后,可通过降低p-ERK的活性,提高抑癌蛋白P53和PTEN的表达,进而抑制肿瘤细胞的增殖活力。第四部分肿瘤相关巨噬细胞介导的VEGFR3对肿瘤药物敏感性的影响目的:探讨肿瘤相关巨噬细胞(TAM)介导的人肺腺癌细胞VEGFR3的表达对肺腺癌A549细胞化疗药物敏感性的影响,为临床肺腺癌的治疗提供新的策略。方法:在体外,用流式细胞分析仪检测VEGFR3抑制剂(MAZ51)与化疗药物多柔比星在不同的药物组合方案中,其共培养液中肺腺癌A549细胞凋亡的情况。在体内,构建裸鼠皮下成瘤模型,检测VEGFR3抑制剂(MAZ51)对肺腺癌化疗药物敏感性的影响。结果:体外实验证实,与单独运用MAZ51、多柔比星或者两者同时运用相比,先用MAZ51处理肿瘤细胞,而后用多柔比星处理肿瘤细胞后,其细胞的凋亡比例比单用MAZ51、多柔比星或者同时运用两种药物更加明显。体内实验证实,与单独运用多柔比星相比,MAZ51与多柔比星联合运用后,其裸鼠肿瘤的生长体积明显降低。结论:抑制VEGFR3后可以增强药物多柔比星对肺腺癌的化疗敏感性。
[Abstract]:Part I: Induction and identification of tumor-associated macrophages Objective: To establish human tumor-associated macrophages (TAM) by induction in vitro and analyze their phenotypic characteristics. Methods: It is generally believed that normal macrophages are M1 macrophages and TAM is M2 macrophages. The expression of CD14 on the surface of macrophages (PMA only type) was detected by flow cytometry, and then the polarization of macrophages was stimulated by human interleukin 4 (IL-4) or lipopolysaccharide (LPS) to M2 or M1 type macrophages respectively. IL-6mR was detected by semi-quantitative RT-PCR. Results: The expression of CD14 on the surface of THP-1 cells was significantly increased after PMA induction. Compared with LPS-induced polarized M 1 macrophages, the expression of IL-6 in PMA-induced macrophages and IL-4-induced M2 macrophages was significantly increased. CONCLUSION: Macrophages were successfully induced to express CD14 over-expression and tend to M2 type macrophages, and TAM was successfully induced, characterized by low expression of IL-6 and TNF-alpha in M2 type macrophages. Objective: To investigate the effect of tumor-associated macrophages (TAM) on the migration and invasion of human lung adenocarcinoma A549 cells, and to analyze its potential molecular mechanism. Methods: TAM and A549 cells were co-cultured indirectly in Transwell chamber, and then the co-culture medium was collected for follow-up. Scratch healing test was used to detect the effect of tumor-associated macrophages (TAM) on the migration of A549 cells; Transwell invasion test was used to detect the effect of tumor-associated macrophages (TAM) on the invasion of A549 cells; semi-quantitative RT-PCR, Q RT-PCR and Western blot were used to detect the expression of VEGF-C, VEGF-D and VEGFR3 in A549 cells. Immunohistochemistry (IHC) was used to detect the expression and distribution of VEGF R3 in lung adenocarcinoma. In tumor-associated macrophage (TAM) microenvironment, the inhibitor of VEGF R3 (MAZ51) inhibited the expression of VEGF R3. Scratch healing test and Transwell invasion test were used to detect the migration and invasion ability of A549 cells. Tumor-associated macrophages (TAM) increased the expression of VEGF-C and VEGFR3 protein in lung adenocarcinoma A549 cells. Immunohistochemistry (IHC) showed that the expression of VEGF-R3 was mainly high in human lung adenocarcinoma cells. Inhibition of VEGF-R3 significantly decreased the migration and invasion of lung adenocarcinoma A549 cells. Cell (TAM) promotes the migration and invasion of tumor cells by increasing the expression of VEGFR3 in A549 cells. Part III Effect of tumor-associated macrophages-mediated VEGFR3 on tumor proliferation Objective: To investigate the effect of tumor-associated macrophages (TAM)-mediated expression of human lung adenocarcinoma cell line VEGFR3 on the proliferation of lung adenocarcinoma cells and explore its potential. Methods: MTT assay was used to analyze the changes of A549 cell viability in co-culture medium with different concentration gradient of inhibitor of VEGF R3 (MAZ51); flow cytometry was used to detect the apoptosis of A549 cells in co-culture with MAZ51; cell colony formation assay was used to analyze the inhibitory effect of MAZ51 on the proliferation of co-cultured A549 cells. Western blot assay was used to detect the expression of MAPK signaling pathway and P53, PTEN, BCL-2 and MMP-2 proteins in A549 cells co-cultured with MAZ51. Semi-quantitative RT-PCR was used to detect the inhibition of ERK signaling pathway by ERK signaling pathway inhibitor U0126 and the interference of SIRNA with P53 and PTEN. After interfering with the expression of P53 and PTEN by Si RNA, A549 cells were co-cultured with MAZ51. Results: MAZ51 decreased the proliferation, apoptosis and colony formation of A549 cells. Western blot showed that MAZ51 could inhibit the activity of p-ERK, increase the expression of tumor suppressor P53 and PTEN, and decrease the number of colony formation. The expression of anti-apoptotic protein BCL-2 and pro-invasive protein MMP-2 was significantly increased after inhibition of ERK signaling pathway. Interference with the expression of P53 and PTEN increased the proliferative activity of co-cultured A549 cells. Conclusion: Inhibition of VEGF R3 can inhibit the expression of tumor suppressor protein P53 and PTEN by decreasing the activity of p-ERK. Part IV Effect of tumor-associated macrophages-mediated VEGF R3 on tumor drug sensitivity Objective: To investigate the effect of tumor-associated macrophages (TAM)-mediated expression of human lung adenocarcinoma cell line VEGF R3 on chemosensitivity of lung adenocarcinoma A549 cells, and to provide a new strategy for clinical treatment of lung adenocarcinoma. In vitro, the apoptosis of lung adenocarcinoma A549 cells was detected by flow cytometry (FCM) in the co-culture medium of the inhibitor of VEGF R3 (MAZ51) and the chemotherapeutic drug doxorubicin in different regimens. In vivo, a nude mouse model of subcutaneous tumor formation was established to detect the effect of the inhibitor of VEGF R3 (MAZ51) on the chemosensitivity of lung adenocarcinoma. Compared with MAZ51, doxorubicin or both, the apoptotic rate of tumor cells treated with MAZ51 and then doxorubicin was more obvious than that treated with MAZ51, doxorubicin or both. Conclusion: Inhibition of VEGF R3 can enhance the chemosensitivity of doxorubicin to lung adenocarcinoma.
【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R734.2
本文编号：2240314
[Abstract]:Part I: Induction and identification of tumor-associated macrophages Objective: To establish human tumor-associated macrophages (TAM) by induction in vitro and analyze their phenotypic characteristics. Methods: It is generally believed that normal macrophages are M1 macrophages and TAM is M2 macrophages. The expression of CD14 on the surface of macrophages (PMA only type) was detected by flow cytometry, and then the polarization of macrophages was stimulated by human interleukin 4 (IL-4) or lipopolysaccharide (LPS) to M2 or M1 type macrophages respectively. IL-6mR was detected by semi-quantitative RT-PCR. Results: The expression of CD14 on the surface of THP-1 cells was significantly increased after PMA induction. Compared with LPS-induced polarized M 1 macrophages, the expression of IL-6 in PMA-induced macrophages and IL-4-induced M2 macrophages was significantly increased. CONCLUSION: Macrophages were successfully induced to express CD14 over-expression and tend to M2 type macrophages, and TAM was successfully induced, characterized by low expression of IL-6 and TNF-alpha in M2 type macrophages. Objective: To investigate the effect of tumor-associated macrophages (TAM) on the migration and invasion of human lung adenocarcinoma A549 cells, and to analyze its potential molecular mechanism. Methods: TAM and A549 cells were co-cultured indirectly in Transwell chamber, and then the co-culture medium was collected for follow-up. Scratch healing test was used to detect the effect of tumor-associated macrophages (TAM) on the migration of A549 cells; Transwell invasion test was used to detect the effect of tumor-associated macrophages (TAM) on the invasion of A549 cells; semi-quantitative RT-PCR, Q RT-PCR and Western blot were used to detect the expression of VEGF-C, VEGF-D and VEGFR3 in A549 cells. Immunohistochemistry (IHC) was used to detect the expression and distribution of VEGF R3 in lung adenocarcinoma. In tumor-associated macrophage (TAM) microenvironment, the inhibitor of VEGF R3 (MAZ51) inhibited the expression of VEGF R3. Scratch healing test and Transwell invasion test were used to detect the migration and invasion ability of A549 cells. Tumor-associated macrophages (TAM) increased the expression of VEGF-C and VEGFR3 protein in lung adenocarcinoma A549 cells. Immunohistochemistry (IHC) showed that the expression of VEGF-R3 was mainly high in human lung adenocarcinoma cells. Inhibition of VEGF-R3 significantly decreased the migration and invasion of lung adenocarcinoma A549 cells. Cell (TAM) promotes the migration and invasion of tumor cells by increasing the expression of VEGFR3 in A549 cells. Part III Effect of tumor-associated macrophages-mediated VEGFR3 on tumor proliferation Objective: To investigate the effect of tumor-associated macrophages (TAM)-mediated expression of human lung adenocarcinoma cell line VEGFR3 on the proliferation of lung adenocarcinoma cells and explore its potential. Methods: MTT assay was used to analyze the changes of A549 cell viability in co-culture medium with different concentration gradient of inhibitor of VEGF R3 (MAZ51); flow cytometry was used to detect the apoptosis of A549 cells in co-culture with MAZ51; cell colony formation assay was used to analyze the inhibitory effect of MAZ51 on the proliferation of co-cultured A549 cells. Western blot assay was used to detect the expression of MAPK signaling pathway and P53, PTEN, BCL-2 and MMP-2 proteins in A549 cells co-cultured with MAZ51. Semi-quantitative RT-PCR was used to detect the inhibition of ERK signaling pathway by ERK signaling pathway inhibitor U0126 and the interference of SIRNA with P53 and PTEN. After interfering with the expression of P53 and PTEN by Si RNA, A549 cells were co-cultured with MAZ51. Results: MAZ51 decreased the proliferation, apoptosis and colony formation of A549 cells. Western blot showed that MAZ51 could inhibit the activity of p-ERK, increase the expression of tumor suppressor P53 and PTEN, and decrease the number of colony formation. The expression of anti-apoptotic protein BCL-2 and pro-invasive protein MMP-2 was significantly increased after inhibition of ERK signaling pathway. Interference with the expression of P53 and PTEN increased the proliferative activity of co-cultured A549 cells. Conclusion: Inhibition of VEGF R3 can inhibit the expression of tumor suppressor protein P53 and PTEN by decreasing the activity of p-ERK. Part IV Effect of tumor-associated macrophages-mediated VEGF R3 on tumor drug sensitivity Objective: To investigate the effect of tumor-associated macrophages (TAM)-mediated expression of human lung adenocarcinoma cell line VEGF R3 on chemosensitivity of lung adenocarcinoma A549 cells, and to provide a new strategy for clinical treatment of lung adenocarcinoma. In vitro, the apoptosis of lung adenocarcinoma A549 cells was detected by flow cytometry (FCM) in the co-culture medium of the inhibitor of VEGF R3 (MAZ51) and the chemotherapeutic drug doxorubicin in different regimens. In vivo, a nude mouse model of subcutaneous tumor formation was established to detect the effect of the inhibitor of VEGF R3 (MAZ51) on the chemosensitivity of lung adenocarcinoma. Compared with MAZ51, doxorubicin or both, the apoptotic rate of tumor cells treated with MAZ51 and then doxorubicin was more obvious than that treated with MAZ51, doxorubicin or both. Conclusion: Inhibition of VEGF R3 can enhance the chemosensitivity of doxorubicin to lung adenocarcinoma.
【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R734.2
【参考文献】
相关期刊论文 前1条
1 许金华;朱文玉;;肺腺癌化疗方案及辅助药物使用分析[J];中国药房;2013年22期
，本文编号：2240314
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