联合检测血清AFP mRNA、h-TERT mRNA、CD44v6 mRNA和MAGE mRNA对原发性肝癌术后复发转移

发布时间：2018-09-14 15:30

【摘要】：目的:通过检测原发性肝癌患者血清AFP mRNA、h-TERT mRNA、CD44v6mRNA和MAGE mRNA表达情况,研究联合检测四种标志物在预测肿瘤术后复发转移中的早期预测价值。方法:采集40例PHC患者、20例活动性肝炎(HBV)患者及20例健康成人外周血,通过RT-PCR技术检测血清AFP mRNA、h TERT mRNA、CD44v6mRNA、MAGE-1 mRNA表达情况。结果:40例PHC患者血清AFP mRNA、h-TERT mRNA、CD44v6 mRNA、MAGE-1 mRNA阳性表达率分别为50%(20/40)、62.5%(25/40)、55%(22/40)、37.5%(15/40),90%的患者至少表达一种标志物。ROC分析显示AFP/h-TERT/CD44v6/MAGE-1 mRNA均阳性表达较AFP mRNA、h-TERT mRNA、CD44v6 mRNA、MAGE-1 mRNA中任一种标志物具有更大的曲线下面积(AUC=0.766),特异性(96.2%),阳性预测值(88.9%),阴性预测值(71.4%)。生存分析表明临床分期、h-TERT mRNA、CD44v6 mRNA、AFP/hTERT/CD44v6/MAGE-1 mRNA与患者术后复发转移相关,进一步logistic回归分析表明,临床分期及AFP/h-TERT/CD44v6/MAGE-1均阳性表达是原发性肝癌患者术后复发的独立预测因素(P0.05)。结论:联合检测AFP mRNA、h-TERT mRNA、CD44v6 mRNA和MAGE mRNA表达提高了术后早期预测PHC患者复发的准确性,可能具有一定的临床应用价值。
[Abstract]:Objective: to study the early predictive value of combined detection of four markers in predicting recurrence and metastasis of primary liver cancer by detecting the expression of serum AFP mRNA,h-TERT mRNA,CD44v6mRNA and MAGE mRNA in patients with primary liver cancer. Methods: peripheral blood samples were collected from 40 patients with PHC and 20 patients with active hepatitis (HBV) and 20 healthy adults. The expression of serum AFP mRNA,h TERT mRNA,CD44v6mRNA,MAGE-1 mRNA was detected by RT-PCR technique. Results the positive expression rate of serum AFP mRNA,h-TERT mRNA,CD44v6 mRNA,MAGE-1 mRNA in 40 PHC patients was 50% (20 / 40), 62.5% (25 / 40) or 55% (22 / 40) or 90% (15 / 40), respectively. (AUC=0.766), specificity (96.2%), positive predictive value (88.9%), negative predictive value (71.4%). Survival analysis showed that the clinical staging of h-TERT mRNA,CD44v6 mRNA,AFP/hTERT/CD44v6/MAGE-1 mRNA was associated with postoperative recurrence and metastasis. Further logistic regression analysis showed that the positive expression of AFP/h-TERT/CD44v6/MAGE-1 and clinical staging were independent predictors of postoperative recurrence in patients with primary liver cancer (P0.05). Conclusion: combined detection of AFP mRNA,h-TERT mRNA,CD44v6 mRNA and MAGE mRNA can improve the accuracy of early prediction of recurrence of PHC patients, and may have some clinical value.
【学位授予单位】：宁波大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R735.7

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