肺腺癌和肺鳞癌中mRNA可变剪接特征的比较

发布时间：2018-10-05 16:56

【摘要】：利用SUPPA(a super-fast pipeline for alternative splicing)软件分析癌症基因组数据库癌基因组图谱中肺腺癌和肺鳞癌样本的RNA-Seq数据,发现在鉴定的7种主要可变剪接转录本中外显子跳跃转录本发生频率最高.通过癌组织与癌旁组织的比较,系统地鉴定了肺腺癌和肺鳞癌中差异表达的可变剪接转录本,发现约60%的差异可变剪接转录本是两种肺癌亚型所共有的.功能富集分析表明,癌组织特异的可变剪接转录本主要富集在细胞周期调控、脱氧核糖核酸代谢和核糖核酸代谢等生物过程中,而癌旁组织特异的可变剪接转录本则主要富集在囊泡介导转运、多肽转运和脂质转运等生物过程中.通过肺癌驱动基因可变剪接转录本的比较分析,鉴定了两种肺癌亚型共有或特有的可能具有癌驱动功能的可变剪接转录本.
[Abstract]:The SUPPA (a super-fast pipeline for alternative splicing) software was used to analyze the RNA-Seq data of lung adenocarcinoma and lung squamous cell carcinoma samples from the cancer genome map of cancer genome database. It was found that the exon jumping transcripts were the most frequent among the 7 major mutable splicing transcripts identified. The variable splicing transcripts of differential expression in lung adenocarcinoma and lung squamous cell carcinoma were systematically identified by comparing cancer tissues with adjacent tissues. It was found that about 60% of the differentially expressed variant splicing transcripts were common to two subtypes of lung cancer. Functional enrichment analysis showed that the specific variable splicing transcripts were mainly concentrated in cell cycle regulation, deoxyribonucleic acid metabolism and ribonucleic acid metabolism, and other biological processes, such as cell cycle regulation, deoxyribonucleic acid metabolism and ribonucleic acid metabolism. The specific variant splicing transcripts of paracancerous tissues were mainly concentrated in vesicular mediated transport, polypeptide transport and lipid transport. Based on the comparative analysis of variant splicing transcripts of lung cancer driving genes, two types of lung cancer subtypes were identified.
【作者单位】：深圳大学医学部呼吸疾病国家重点实验室深圳大学变态反应分室;中国科学院遗传与发育生物学研究所;
【基金】：国家高技术研究发展计划资助项目(2012AA020409) 深圳市知识创新计划基础研究资助项目(JCYJ20150525092941055)~~
【分类号】：R734.2

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