甲状腺未分化癌组织vWF表达与血管生成拟态相关性研究

发布时间：2018-10-05 20:25

【摘要】：目的肿瘤源性的血管性血友病因子(von Willbrend factor,vWF)表达及血管生成拟态(vasculogenic mimicry,VM)形成都是高度恶性肿瘤中的高频事件。本研究检测甲状腺未分化癌组织中vWF的表达及VM水平,在组织学水平上探讨vWF的表达及其与肿瘤组织中VM形成的相关性。方法选取甘肃省人民医院(20例)及甘肃省肿瘤医院(43例)2000-01-01-2014-12-31病理诊断为甲状腺未分化癌的手术标本63例。同时随机挑选甘肃省人民医院同期甲状腺乳头状癌病例63例和相应癌旁正常组织作为对照。采用免疫组化法检测甲状腺组织中vWF蛋白的表达水平,CD31联合PAS双重染色检测VM水平。结果vWF蛋白在正常甲状腺组织无表达,甲状腺乳头状癌中有5例(7.90%)阳性表达,甲状腺未分化癌中vWF阳性表达31例(49.20%),组间比较差异有统计学意义,χ~2=26.289,P0.001。vWF在甲状腺未分化癌中的阳性表达与患者的性别、年龄、肿瘤大小和淋巴结转移无关,而与远处转移(χ~2=5.341,P=0.021)和临床分期(χ~2=4.753,P=0.029)相关。VM在正常甲状腺组织、乳头状癌及甲状腺未分化癌中的形成率分别为0、3.20%和34.92%,组间差异有统计学意义,χ~2=20.588,P0.001。VM形成与肿瘤最大径(χ~2=4.789,P=0.029)、淋巴结转移(χ~2=5.684,P=0.028)、远处转移(χ~2=4.722,P=0.030)和临床分期(χ~2=5.777,P=0.061)相关。vWF的表达与VM水平正相关,r=0.411,P=0.001。结论甲状腺未分化癌中有不同程度vWF的高表达,其高表达与VM的形成正相关,vWF的高表达可能是导致甲状腺未分化癌预后差的因素之一。
[Abstract]:Objective the expression of tumor derived von Willebrand factor (von Willbrend factor,vWF) and the formation of angiogenic mimicry (vasculogenic mimicry,VM) are high frequency events in highly malignant tumors. In this study, the expression of vWF and the level of VM were detected in undifferentiated thyroid carcinoma. The expression of vWF and its correlation with the formation of VM in tumor tissue were studied at the histological level. Methods Sixty-three cases of undifferentiated thyroid carcinoma were selected from Gansu Provincial people's Hospital (20 cases) and Gansu Cancer Hospital (43 cases) from January to January 2014-12-31, 2000. At the same time, 63 cases of thyroid papillary carcinoma in Gansu Provincial people's Hospital were randomly selected as control group. The expression of vWF protein in thyroid tissue was detected by immunohistochemical method and the VM level was detected by double staining of CD31 and PAS. Results there was no expression of vWF protein in normal thyroid tissue, and 5 cases (7.90%) were positive in papillary thyroid carcinoma. 31 cases (49.20%) of thyroid undifferentiated carcinoma showed vWF positive expression. The positive expression of vWF in undifferentiated thyroid carcinoma was not correlated with sex, age, tumor size and lymph node metastasis. VM was associated with distant metastasis (蠂 2 + 5.341) and clinical stage (蠂 2 / 24.753) in normal thyroid tissue. The formation rates of papillary carcinoma and thyroid undifferentiated carcinoma were 0 / 3.20% and 34.92%, respectively. There was significant difference between the two groups. The expression of VWF was positively correlated with the VM level and the maximum diameter of the tumor (蠂 2 / 24.789 / P 0.029), lymph node metastasis (蠂 ~ 2 / 25.684P / 0.028), distant metastasis (蠂 ~ 2 / 2 / 4.722 / P ~ 0.030) and clinical stage (蠂 ~ 25.777A / P 0.061). The expression of VWF was positively correlated with the VM level. Conclusion there is high expression of vWF in undifferentiated thyroid carcinoma. The high expression of vWF and the formation of VM may be one of the factors leading to poor prognosis of undifferentiated thyroid carcinoma.
【作者单位】：甘肃省人民医院病理科;甘肃省肿瘤医院病理科;
【分类号】：R736.1

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