Trim28、PRR11在结肠癌中的发生、发展及其临床意义

发布时间：2018-10-10 10:40

【摘要】：目的:探析Trim28、PRR11在结肠癌中的表达及其发生、发展中的意义,对Trim28、PRR11在结肠癌临床诊断中的表达价值进行评价,为临床诊断和治疗提供参考依据。方法:此次研究纳入97例结肠癌患者作为主要研究对象,病例纳入时间为2015年9月~2016年10月。选择97例结肠癌患者的肿瘤组织、及其中34例结肠癌患者距肿瘤边缘6cm以上的正常组织为主要研究内容。对肿瘤病理组织和正常组织进行免疫组化检测,检测两种组织中的Trim28、PRR11,对Trim28、PRR11与结肠癌临床病理组织相关参数的关系进行对比分析。结果:(1)Trim28、PRR11阳性表达率:结肠癌组织的Trim28的阳性表达率为94.85%,PRR11的阳性表达率为91.75%,正常组织的Trim28的阳性表达率为26.47%,正常组织中PRR11的阳性表达率为38.24%。通过统计学分析,发现两种指标的病理组织和正常组织对比差异显著,P0.05,存在统计学意义。(2)相关性上:在相关性方面:Trim28的表达与结肠癌肿瘤的肿瘤大小、TNM分期、肿瘤分化程度上存在明显相关性(P0.05);PRR11的表达与结肠癌肿瘤的TNM分期、肿瘤分化程度上存在明显相关性(P0.05);结肠癌病理组织中Trim28、PRR11的表达情况与患者的性别、年龄无明显相关性(P0.05)。(3)Trim28、PRR11表达对结肠癌细胞增殖的影响:结肠癌病理组织的细胞增殖OD值明显低于正常组织,组间差异存在统计学意义(P0.05)。(4)Trim28、PRR11表达对结肠癌细胞迁移的影响:结肠癌病理组织的细胞迁移距离明显低于正常组织,经统计学分析,P0.05,组间差异较为显著。结论:结肠癌中Trim28、PRR11的高表达与结肠癌的发生、恶性肿瘤的发展行为均存在明显相关性,可为临床诊断和疾病进展提供判断依据。降低结肠癌组织中Trim28、PRR11的高表达,可抑制结肠癌细胞增殖、迁移,是结肠癌治疗的靶点,同时也是对结肠癌患者进行预后判定的主要指标之一。
[Abstract]:Objective: to explore the expression and development of Trim28,PRR11 in colon cancer and evaluate the value of Trim28,PRR11 expression in the clinical diagnosis of colon cancer so as to provide reference for clinical diagnosis and treatment. Methods: 97 patients with colon cancer were included in the study from September 2015 to October 2016. The tumor tissues of 97 patients with colon cancer and 34 normal tissues above the margin of tumor were selected as the main contents of the study. The relationship between Trim28,PRR11 and clinicopathological parameters of colon cancer was compared and analyzed by using Trim28,PRR11, in tumor tissues and normal tissues. Results: (1) the positive expression rate of Trim28,PRR11: the positive expression rate of Trim28 in colon cancer tissue was 94.85 and the positive rate of PRR11 was 91.755.The positive expression rate of Trim28 in normal tissue was 26.47 and the positive expression rate of PRR11 in normal tissue was 38.24. Through statistical analysis, we found that there was significant difference between pathological tissue and normal tissue of the two indexes (P 0.05). (2) correlation: the expression of 1: Trim28 was correlated with the tumor size and TNM stage of colon cancer tumor. There was a significant correlation between the expression of PRR11 and the TNM stage of colon cancer tumor and the degree of tumor differentiation (P0.05). The expression of Trim28,PRR11 in the pathological tissue of colon cancer was correlated with the sex of the patient. There was no significant correlation of age (P0.05). (3) Trim28,PRR11 expression on the proliferation of colon cancer cells: the OD value of cell proliferation in pathological tissues of colon cancer was significantly lower than that in normal tissues. There was significant difference between the groups (P0.05). (4) Trim28,PRR11 expression on the migration of colon cancer cells: the distance of cell migration in pathological tissues of colon cancer was significantly lower than that in normal tissues (P0.05), the difference between groups was more significant. Conclusion: the high expression of Trim28,PRR11 in colon cancer has a significant correlation with the occurrence of colon cancer and the development of malignant tumor, which can provide a basis for clinical diagnosis and disease progression. Reducing the high expression of Trim28,PRR11 in colon cancer tissue can inhibit the proliferation and migration of colon cancer cells. It is the target of colon cancer therapy and one of the main prognostic indicators for colon cancer patients.
【学位授予单位】：承德医学院
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R735.35

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