Notch2、Notch3在非小细胞肺癌中的表达及相关性研究
发布时间：2018-10-15 08:43
【摘要】：目的:研究Notch2和Notch3在非小细胞肺癌(NSCLC)中的表达,以及它们和相关临床参数(包括性别、年龄、组织类型、TNM分期、肿瘤分化程度、淋巴结转移、原发灶大小、原发肿瘤状态)之间的联系,从而探讨Notch2和Notch3在非小细胞肺癌中表达的临床意义,探寻能用于估测NSCLC病人生存预后的新型分子标记物,以及NSCLC靶向治疗的新靶点。方法:挑选手术后病理证实的非小细胞肺癌病人癌组织标本60例,并且取30例癌旁正常肺组织当作对照。通过制作石蜡组织切片、常规HE染色、免疫组织化学染色,运用免疫组化方法,观察Notch2、Notch3在非小细胞肺癌组织和癌旁正常肺组织中的表达情况。结果:1.Notch2主要表达于肿瘤细胞的细胞质,Notch2在非小细胞肺癌组织中的表达水平大大高于癌旁肺组织(P0.05);Notch2表达与非小细胞肺癌病人的性别、年龄、组织类型及原发灶大小无明显相关性(P0.05);Notch2阳性表达率在Ⅲ、Ⅳ期NSCLC患者中明显高于Ⅰ、Ⅱ期患者(P0.05),低分化患者显然高于高分化患者(P0.05),有淋巴结转移患者明显高于无淋巴结转移患者(P0.05),原发肿瘤T3、T4期患者明显高于T1、T2期患者(P0.05);2.Notch3亦主要表达于细胞质,Notch3在非小细胞肺癌组织中的表达率大大高于癌旁肺组织,其阳性表达率在Ⅲ、Ⅳ期NSCLC患者中明显高于Ⅰ、Ⅱ期患者,低分化患者显然高于高分化患者,有淋巴结转移患者明显高于无淋巴结转移患者,原发肿瘤T3、T4期患者明显高于T1、T2期患者,上述差异均具有统计学意义(P0.05);Notch3的表达与非小细胞肺癌病人的性别、年龄、组织类型及原发灶大小也无明显相关性(P0.05);3.Notch2、Notch3在NSCLC中的表达呈正相关(P0.05)。结论:Notch2和Notch3在非小细胞肺癌组织中异常表达升高,Notch2和Notch3的表达与非小细胞肺癌临床分期、肿瘤分化程度、原发肿瘤状态、有无淋巴结转移紧密相关,在非小细胞肺癌的发生发展中发挥作用,在评估非小细胞肺癌病人的生存预后方面有一定的参考和指导意义。
[Abstract]:Objective: to investigate the expression of Notch2 and Notch3 in non-small cell lung cancer (NSCLC) (NSCLC), as well as their clinical parameters (including sex, age, tissue type, TNM stage, tumor differentiation, lymph node metastasis, primary tumor size). To explore the clinical significance of the expression of Notch2 and Notch3 in non-small cell lung cancer (NSCLC) and to explore a new molecular marker for estimating the survival and prognosis of NSCLC patients and a new target for NSCLC targeted therapy. Methods: 60 patients with non-small cell lung cancer and 30 normal adjacent lung tissues were selected as control group. The expression of Notch2,Notch3 in non-small cell lung cancer (NSCLC) and adjacent normal lung tissues (NSCLC) was observed by paraffin section, routine HE staining and immunohistochemical staining. Results: 1.Notch2 was mainly expressed in the cytoplasm of tumor cells. The expression of Notch2 in NSCLC was significantly higher than that in adjacent lung tissues (P0.05), and the expression of Notch2 was correlated with the sex and age of NSCLC patients. There was no significant correlation between the histological type and the size of the primary tumor (P0.05), and the positive expression rate of Notch2 was significantly higher in the patients with stage 鈪，
本文编号：2271988
[Abstract]:Objective: to investigate the expression of Notch2 and Notch3 in non-small cell lung cancer (NSCLC) (NSCLC), as well as their clinical parameters (including sex, age, tissue type, TNM stage, tumor differentiation, lymph node metastasis, primary tumor size). To explore the clinical significance of the expression of Notch2 and Notch3 in non-small cell lung cancer (NSCLC) and to explore a new molecular marker for estimating the survival and prognosis of NSCLC patients and a new target for NSCLC targeted therapy. Methods: 60 patients with non-small cell lung cancer and 30 normal adjacent lung tissues were selected as control group. The expression of Notch2,Notch3 in non-small cell lung cancer (NSCLC) and adjacent normal lung tissues (NSCLC) was observed by paraffin section, routine HE staining and immunohistochemical staining. Results: 1.Notch2 was mainly expressed in the cytoplasm of tumor cells. The expression of Notch2 in NSCLC was significantly higher than that in adjacent lung tissues (P0.05), and the expression of Notch2 was correlated with the sex and age of NSCLC patients. There was no significant correlation between the histological type and the size of the primary tumor (P0.05), and the positive expression rate of Notch2 was significantly higher in the patients with stage 鈪，
本文编号：2271988
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