氧化石墨烯介导的光热免疫疗法治疗转移性小鼠乳腺肿瘤
发布时间：2018-11-05 18:40
【摘要】：本研究分别从细胞层面和在体层面,研究纳米氧化石墨烯(nano graphene oxide,NGO)联合805 nm近红外激光对于肿瘤的杀伤和免疫刺激作用.细胞实验证实NGO具有良好的光热转换效应,联合激光治疗,能够有效杀伤肿瘤细胞.此外,NGO能够刺激巨噬细胞产生IL-6及TNFα,并增强激光杀伤的肿瘤细胞对巨噬细胞的免疫刺激效应.在体实验中,在小鼠背部不同位置使用同一瘤株种植两个瘤块,模拟局部原发肿瘤和转移瘤.实验结果表明,NGO联合激光治疗可以有效消融局部肿瘤,并且,未治疗的远隔部位肿瘤生长速度也显著降低.说明NGO联合激光治疗的方式在局部杀伤原位肿瘤的同时,可能诱发了机体的抗肿瘤免疫反应,从而达到了抑制远隔肿瘤生长的效果.
[Abstract]:In this study, the cytotoxic and immunostimulatory effects of nano-graphene oxide (nano graphene oxide,NGO) combined with 805 nm near infrared laser on tumor were studied at the cellular and in vivo levels, respectively. Cell experiments have proved that NGO has a good photothermal conversion effect, combined with laser therapy, can effectively kill tumor cells. In addition, NGO stimulated macrophages to produce IL-6 and TNF 伪, and enhanced the immunostimulatory effect of laser killed tumor cells on macrophages. In vivo, two tumor blocks were implanted with the same tumor strain at different sites in the back of mice to simulate local primary tumor and metastatic tumor. The experimental results show that NGO combined with laser therapy can effectively ablation local tumors, and the growth rate of untreated distant tumors is also significantly reduced. The results suggest that the combination of NGO and laser therapy may induce antitumor immune response and inhibit the growth of distant tumor.
【作者单位】： 天津医科大学肿瘤医院 介入肿瘤治疗科 天津市肿瘤防治重点实验室;Biophotonics
【基金】：美国国家卫生研究院(RS20132225-106,国家研究资源中心的INBRE项目) 俄克拉荷马州科学技术促进中心(HR16-085)资助项目~~
【分类号】：R737.9
本文编号：2313013
[Abstract]:In this study, the cytotoxic and immunostimulatory effects of nano-graphene oxide (nano graphene oxide,NGO) combined with 805 nm near infrared laser on tumor were studied at the cellular and in vivo levels, respectively. Cell experiments have proved that NGO has a good photothermal conversion effect, combined with laser therapy, can effectively kill tumor cells. In addition, NGO stimulated macrophages to produce IL-6 and TNF 伪, and enhanced the immunostimulatory effect of laser killed tumor cells on macrophages. In vivo, two tumor blocks were implanted with the same tumor strain at different sites in the back of mice to simulate local primary tumor and metastatic tumor. The experimental results show that NGO combined with laser therapy can effectively ablation local tumors, and the growth rate of untreated distant tumors is also significantly reduced. The results suggest that the combination of NGO and laser therapy may induce antitumor immune response and inhibit the growth of distant tumor.
【作者单位】： 天津医科大学肿瘤医院 介入肿瘤治疗科 天津市肿瘤防治重点实验室;Biophotonics
【基金】：美国国家卫生研究院(RS20132225-106,国家研究资源中心的INBRE项目) 俄克拉荷马州科学技术促进中心(HR16-085)资助项目~~
【分类号】：R737.9
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