TIM-3在胃癌肿瘤浸润γδT细胞与NK细胞中的表达及其临床意义

发布时间：2018-11-05 18:15

【摘要】：检测T细胞免疫球蛋白及黏蛋白分子3(T cell immunoglobulin and mucin domain containing molecules 3,TIM-3)在胃癌患者固有免疫细胞(γδT细胞与NK细胞)中的表达率,探讨其与临床病理特征及术后中位无进展生存期(progress free survival,PFS)的关系。收集临床病理资料齐全的胃癌患者26例,经流式细胞技术检测TIM-3在胃癌组织、癌旁组织及外周血γδT细胞、NK细胞中的表达率,并分析其与临床病理因素及中位PFS的相关性。结果发现,TIM-3在胃癌组织和癌旁组织中γδT细胞上均表达,而2类组织中γδT细胞上TIM-3的表达率差异无统计学意义(P=0.074),但均显著高于其在外周血γδT细胞上的表达水平(P0.001;P0.001)。TIM-3在外周血NK细胞中表达率较高,且TIM-3在外周血中NK细胞表达率显著高于胃癌组织及癌旁组织(P0.001;P0.001),而癌组织与癌旁组织之间差异无统计学意义(P=0.097)。NK细胞中TIM-3的表达率分别与肿瘤浸润深度和TNM分期相关(P0.01),γδT细胞中TIM-3表达率与TNM分期有关(P0.01)。γδT细胞及NK细胞中TIM-3表达率较低的胃癌患者比TIM-3表达率较高的胃癌患者的中位PFS更长(P=0.014;P=0.028),提示TIM-3在胃癌的进展过程中起到重要作用。
[Abstract]:The expression rate of T cell immunoglobulin and mucin molecule 3 (T cell immunoglobulin and mucin domain containing molecules 3 TIM-3 in innate immune cells (纬未 T cells and NK cells) of patients with gastric cancer was detected. To investigate its relationship with clinicopathological features and median progressive survival time (progress free survival,PFS). 26 cases of gastric cancer with complete clinicopathological data were collected. The expression rate of TIM-3 in gastric cancer tissues, peripheral blood 纬未 T cells and NK cells was detected by flow cytometry, and the correlation with clinicopathological factors and median PFS was analyzed. The results showed that TIM-3 was expressed on 纬未 T cells in gastric cancer and paracancerous tissues, but there was no significant difference in the expression rate of TIM-3 on 纬未 T cells between the two types of tissues (P0. 074). The expression level of 纬未 T cells was significantly higher than that of 纬未 T cells (P0.001). The expression rate of TIM-3 in peripheral blood NK cells was higher than that in gastric cancer tissues and adjacent tissues (P0.001), and the expression rate of TIM-3 in peripheral blood NK cells was significantly higher than that in gastric cancer tissues and adjacent tissues (P0.001). There was no significant difference between cancer tissue and paracancerous tissue (P0. 097). NK cell TIM-3 expression was correlated with the depth of tumor invasion and TNM stage (P0. 01), respectively), but there was no significant difference between P0. 001 and paracancerous tissues (P0. 097). The expression rate of TIM-3 in 纬未 T cells was related to the TNM stage (P0.01). The median PFS of patients with lower TIM-3 expression in 纬未 T cells and NK cells was longer than that in patients with higher TIM-3 expression rate (P0.014). It suggests that TIM-3 plays an important role in the progression of gastric cancer.
【作者单位】：苏州大学附属第三医院肿瘤科;江苏省肿瘤免疫治疗工程技术研究中心;
【基金】：国家科技支撑项目(2015BAI12B12) 国家自然科学基金资助项目(31570877;31570908;81171653;30950022);国家自然科学基金海外及港澳学者合作研究基金资助项目(31428005) 江苏省重点研发计划(BE2015634) 常州市卫生系领军人才计划项目(2016ZCLJ022)
【分类号】：R735.2

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