miR-31通过激活NF-κB信号通路而促进结肠癌细胞增殖
发布时间：2018-11-07 21:06
【摘要】：微小RNA(microRNA)在肿瘤的发生发展中发挥重要作用。有研究表明,在结肠癌患者肿瘤组织中,miR-31表达水平增高。然而,定量PCR只能检测所在组织miR-31的整体表达水平,而无法观察miR-31在特定组织与特定细胞中的表达分布。目前,尚未见关于miR-31在结肠癌中原位表达的报道。本文从研究miR-31在结肠癌中的原位表达入手,进一步探究miR-31在结肠癌细胞中的功能及作用机制。原位杂交实验结果显示,miR-31在结肠癌肿瘤细胞中的原位表达明显升高;体外过表达或敲减miR-31证实,其可以促进结肠癌细胞增殖和集落形成;荧光定量PCR与Western印迹和双荧光素酶报告基因实验证实,在结肠癌细胞中,NF-κB通路的抑制因子丝氨酸/苏氨酸激酶40(STK40)是miR-31下游靶基因,miR-31靶向作用于STK40而激活NF-κB通路;反之,抑制NF-κB通路,miR-31的促增殖能力明显下降。上述结果提示,miR-31可能通过激活NF-κB信号通路而促进结肠癌的细胞增殖。
[Abstract]:Micro RNA (microRNA) plays an important role in the development of tumor. Studies have shown that the expression of miR-31 is higher in cancer tissues of colon cancer patients. However, quantitative PCR can only detect the overall expression of miR-31 in tissues, but can not observe the distribution of miR-31 in specific tissues and cells. At present, there is no report on the expression of miR-31 in colon cancer. In order to explore the function and mechanism of miR-31 in colon cancer cells, we studied the in situ expression of miR-31 in colon cancer cells. The results of in situ hybridization showed that the in situ expression of miR-31 was significantly increased in colon cancer cells, and the overexpression or knockdown of miR-31 in vitro confirmed that it could promote the proliferation and colony formation of colon cancer cells. Fluorescence quantitative PCR, Western blot and double luciferase reporter gene experiments confirmed that the inhibitor of NF- 魏 B pathway, serine / threonine kinase 40 (STK40), was the downstream target gene of miR-31 in colon cancer cells. MiR-31 targets STK40 and activates NF- 魏 B pathway. On the contrary, inhibition of NF- 魏 B pathway significantly decreased the proliferative ability of miR-31. These results suggest that miR-31 may promote the proliferation of colon cancer cells by activating NF- 魏 B signaling pathway.
【作者单位】： 北京大学基础医学院生物化学与分子生物学系;北京大学第三医院肿瘤放疗科;北京大学基础医学院病理学系;北京大学第三医院中心实验室;
【基金】：国家自然科学基金(No.81672091,No.81541142) 北京市自然科学基金(No.7172232)资助~~
【分类号】：R735.35
[Abstract]:Micro RNA (microRNA) plays an important role in the development of tumor. Studies have shown that the expression of miR-31 is higher in cancer tissues of colon cancer patients. However, quantitative PCR can only detect the overall expression of miR-31 in tissues, but can not observe the distribution of miR-31 in specific tissues and cells. At present, there is no report on the expression of miR-31 in colon cancer. In order to explore the function and mechanism of miR-31 in colon cancer cells, we studied the in situ expression of miR-31 in colon cancer cells. The results of in situ hybridization showed that the in situ expression of miR-31 was significantly increased in colon cancer cells, and the overexpression or knockdown of miR-31 in vitro confirmed that it could promote the proliferation and colony formation of colon cancer cells. Fluorescence quantitative PCR, Western blot and double luciferase reporter gene experiments confirmed that the inhibitor of NF- 魏 B pathway, serine / threonine kinase 40 (STK40), was the downstream target gene of miR-31 in colon cancer cells. MiR-31 targets STK40 and activates NF- 魏 B pathway. On the contrary, inhibition of NF- 魏 B pathway significantly decreased the proliferative ability of miR-31. These results suggest that miR-31 may promote the proliferation of colon cancer cells by activating NF- 魏 B signaling pathway.
【作者单位】： 北京大学基础医学院生物化学与分子生物学系;北京大学第三医院肿瘤放疗科;北京大学基础医学院病理学系;北京大学第三医院中心实验室;
【基金】：国家自然科学基金(No.81672091,No.81541142) 北京市自然科学基金(No.7172232)资助~~
【分类号】：R735.35
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