同步原位检测CTC多重瘤标蛋白表达与染色体异倍体的意义及应用

发布时间：2018-11-16 18:46

【摘要】：循环肿瘤细胞(circulating tumor cell,CTC)及播散肿瘤细胞(disseminated tumor cell,DTC)的可靠检测主要取决于有效的细胞分离与鉴别,目前最为新颖的"差相富集-多重瘤标免疫荧光染色-染色体荧光原位杂交(SE-i FISH)"技术平台,首次将CTC/DTC的富集方法与后续的原位、同步检测肿瘤细胞染色体异倍体的核型分析及多种肿瘤标志物的表型分析等进行有效整合,并成功应用于肿瘤细胞的基础分析与临床检测。大量临床实验证明,独特的SE-i FISH可以为分析CTC/DTC在肿瘤早期诊断、疗效快速评估、肿瘤耐药与复发的实时监测等提供可靠的技术保障。该文阐述SEi FISH在不同瘤种患者及人源肿瘤动物模型体内的有效检测方法,并锁定具有不同临床意义的不同表型与核型的CTC/DTC亚类细胞,为后续的肿瘤单细胞分析与实验提供指导。
[Abstract]:The reliable detection of circulating tumor cells (circulating tumor cell,CTC) and disseminated tumor cells (disseminated tumor cell,DTC) mainly depends on effective cell isolation and differentiation. At present, the most novel technique of "differential phase enrichment-multiple tumor labeled immunofluorescence staining-chromosome fluorescence in situ hybridization (SE-i FISH)" platform, the enrichment method of CTC/DTC and subsequent in situ for the first time, The karyotype analysis of chromosome aneuploidy and phenotypic analysis of various tumor markers were effectively integrated and successfully applied to the basic analysis and clinical detection of tumor cells. A large number of clinical experiments have proved that the unique SE-i FISH can provide reliable technical support for the analysis of CTC/DTC in early diagnosis of tumor, rapid evaluation of curative effect, real-time monitoring of drug resistance and recurrence of tumor. This paper presents an effective method for the detection of SEi FISH in different tumor patients and human tumor animal models, and localizes CTC/DTC subtypes with different phenotypes and karyotypes with different clinical significance. To provide guidance for the subsequent single cell analysis and experiment of tumor.
【作者单位】：北京协和医院基本外科;中国医学科学院基础医学研究所/北京协和医学院基础学院生物化学与分子生物学系及中心实验室;北京大学人民医院病理科;北京博雅医学健康研究所;北京大学肿瘤医院消化内科;北京大学第一医院乳腺癌中心;中国人民解放军总医院(301医院)肝胆中心;上海第一人民医院肿瘤中心;赛特生物医药科技有限公司;Cytelligen San
【分类号】：R730.4

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