常见肿瘤标志物的生物学变异的研究

发布时间：2018-11-18 07:32

【摘要】：[目的]研究10项常见肿瘤标志物:癌胚抗原、糖类抗原19-9、糖类抗原72-4、甲胎蛋白、绒毛膜促性腺激素、糖类抗原15-3、糖类抗原125、神经元特异性烯醇化酶、细胞角蛋白19片段和总前列腺抗原的生物学变异。[方法]依据美国临床实验室标准化研究所相关评价文件对ROCHE e601电化学发光分析仪进行校准及方法学性能进行评价;通过问卷调查,选取120位健康成年人建立适合于本实验室的上述肿瘤标志物的参考区间;并募集55位健康成年人志愿者,采用相关生物学变异文献的实验方法,研究55例健康成年人志愿者的10项常见肿瘤标志物的个体内生物学变异、个体间生物学变异、参考变化值及个体指数。[结果](1)得出了 10项常见肿瘤标志物的个体内生物学变异、个体间生物学变异、参考变化值及个体指数数据。(2)不同检验项目生物学变异不同,健康成年人志愿者10项常见肿瘤标志物的平均个体内生物学变异,其中CA15-3、AFP等较小,NSE、CA21-1等较大,其余检验项目介于两者之间。(3)健康成年人志愿者10项常见肿瘤标志物个体间生物学变异,其中CA153较小,CA19-9较大,其余检验项目介于两者之间。(4)健康成年人志愿者10项常见肿瘤标志物的参考变化值差异较大。(5)健康成年人志愿者各项肿瘤标志物的个体指数比较接近。[结论](1)本研究得出10种常见肿瘤标志物项目在我国实验室情况下的基于国人的生物学变异数据库。(2)建立的基于中国人群的常见肿瘤标志物的生物学变异数据,与西班牙临床化学和分子病理学协会发布的数据库基本一致,但也有少部分项目存在差异。(3)使用生物学变异数据对于评估肿瘤标志物的群体参考区间、参考变化值及临床诊疗应用有重要价值。
[Abstract]:[objective] to study 10 common tumor markers: carcinoembryonic antigen, carbohydrate antigen 19-9, carbohydrate antigen 72-4, alpha-fetoprotein, chorionic gonadotropin, carbohydrate antigen 15-3, carbohydrate antigen 125, neuron-specific enolase. Biological variation of cytokeratin 19 fragment and total prostate antigen. [methods] the calibration and methodological performance of ROCHE e601 electroluminescent analyzer were evaluated according to the relevant evaluation documents of American Institute of Clinical Laboratory Standardization. By questionnaire, 120 healthy adults were selected to establish a reference range for the above tumor markers in our laboratory. In addition, 55 healthy adult volunteers were recruited to study the intra-individual biological variation and inter-individual biological variation of 10 common tumor markers in 55 healthy adult volunteers. Reference value and individual index. [results] (1) Intra-individual biological variation, inter-individual biological variation, reference variation value and individual index data of 10 common tumor markers were obtained. (2) the biological variation of different test items was different. The average in vivo biological variation of 10 common tumor markers in healthy adult volunteers was CA15-3,AFP and NSE,CA21-1. The other test items were between the two. (3) the biological variation of 10 common tumor markers in healthy adult volunteers, in which CA153 was small, CA19-9 was larger, The other test items were between the two. (4) the reference values of 10 common tumor markers in healthy adult volunteers were significantly different. (5) the individual index of each tumor marker in healthy adult volunteers was similar. [conclusion] (1) in this study, we obtained the biological variation database of 10 common tumor markers in Chinese laboratory. (2) the biological variation data of common tumor markers based on Chinese population were established. It is basically consistent with the database published by the Spanish Association of Clinical Chemistry and Molecular Pathology, but there are also differences in a small number of items. (3) the use of biological variation data to evaluate the population reference interval for tumor markers. Reference value and clinical application have important value.
【学位授予单位】：昆明医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R730.43

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