白藜芦醇对胃癌细胞及其表达DNA甲基转移酶和组蛋白脱乙酰化酶的影响及意义

发布时间：2018-11-17 21:03

【摘要】：目的:探讨白藜芦醇(resveratrol,Res)对AGS胃癌细胞及其表达DNA甲基转移酶(DNA methyltransferase,DNMTs)和组蛋白脱乙酰化酶(Histone deacetylase,HDACs)的影响及意义。方法:将培养好的胃癌细胞分别用浓度为空白、25μmol/L、50μmol/L、100μmol/L的Res配置的培养基培养24小时后分别用流式仪测验AGS的凋亡及周期阻滞状态;免疫细胞化学测DNMT1、DNMT3a、DNMT3b、HDAC2、HDAC11表达情况。结果:培养24h后对照组、25μmol/L组、50μmol/L组、100μmol/L组用流式细胞仪测凋亡结果分别为(1.48±0.15、12.77±0.26、26.59±0.43、38.59±0.23,F=9585.25,p0.01);流式细胞仪测细胞周期G1期结果分别为(44.47±0.41、52.15±0.34、55.22±0.32、71.48±0.40,F=2807.43,p0.01);免疫细胞化学检测DNMTs和HDACs灰度值结果分别是DNMT1(191.70±0.60、197.37±0.73、201.88±0.29、207.97±0.81,F=347.21,p0.01)、DNMT3a(187.64±0.96、197.21±0.63、203.35±0.74、208.17±0.30,F=481.49,p0.01)、DNMT3b(191.69±0.65、196.36±0.44、201.04±0.37、205.69±0.46,F=452.17,p0.01)、HDAC2(184.58±1.01、193.32±0.57、200.60±0.84、205.12±0.53,F=414.68,p0.01)、HDAC11(189.94±0.70、194.14±0.99、201.71±0.73、207.46±0.77,F=445.69,p0.01)。结论:1.一定浓度范围内,Res能够抑制DNMTs和HDACs的产生,并加快胃癌细胞凋亡,并且可以将周期阻滞于G1期;2.Res发挥抗肿瘤作用存在一定的浓度相关性。
[Abstract]:Aim: to investigate the effect and significance of resveratrol (resveratrol,Res) on the expression of DNA methyltransferase (DNA methyltransferase,DNMTs) and histone deacetylase (Histone deacetylase,HDACs) in AGS gastric cancer cells. Methods: the cultured gastric cancer cells were cultured on the Res medium with 25 渭 mol/L,50 渭 mol/L,100 渭 mol/L for 24 hours. The apoptosis and cycle arrest of AGS were measured by flow cytometry. The expression of DNMT1,DNMT3a,DNMT3b,HDAC2,HDAC11 was detected by immunocytochemistry. Results: 24 hours after culture, the apoptosis of the control group, 25 渭 mol/L group, 50 渭 mol/L group and 100 渭 mol/L group were measured by flow cytometry (1.48 卤0.151.77 卤0.260.26 卤26.59 卤0.43) respectively. The G1 phase of cell cycle was measured by flow cytometry (44.47 卤0.41U 52.15 卤0.34U 55.22 卤0.32U 71.48 卤0.40F 2807.43p0.01). The gray values of DNMTs and HDACs by immunocytochemistry were DNMT1 (191.70 卤0.60197.37 卤0.73201.88 卤0.29207.97 卤0.81FU 347.21 p0.01) and DNMT3a (187.64 卤0.96197.21 卤0.63203.35 卤0.74208.17 卤0.30F481.49p0.01), respectively. DNMT3b (191.69 卤0.65196.36 卤0.44201.04 卤0.37205.69 卤0.46FU 452.17p0.01), HDAC2 (184.58 卤1.01193.32 卤0.57200.60 卤0.84205.12 卤0.53FU 414.68p0.01). HDAC11 (189.94 卤0.70194.14 卤0.99201.71 卤0.73207.46 卤0.777). Conclusion: 1. In a certain concentration range, Res could inhibit the production of DNMTs and HDACs, accelerate the apoptosis of gastric cancer cells, and block the cell cycle in G1 phase.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R735.2

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