基于穿刺活检的复发转移性结直肠癌患者源性异种移植模型的建立

发布时间：2018-11-22 12:28

【摘要】：背景与目的:现行的结直肠癌患者源性异种移植(patient-derived xenografts,PDXs)模型建模样本多来源于外科手术,但复发转移性结直肠癌(metastatic colorectal cancer,m CRC)患者手术机会少,难以获取标本。该研究旨在利用穿刺活检术建立m CRC的PDXs模型。方法:入组12例结直肠癌根治术后患者,存在临床症状和(或)影像学提示术后复发和(或)转移,需行穿刺活检明确诊断、且不存在穿刺活检禁忌证者,保留用于常规病理诊断包括基因检测的组织量后,剩余的标本用于PDXs模型的建立。结果:共成功建立7例m CRC的PDXs模型,成功率为77.8%。结论:基于穿刺活检术建立m CRC的PDXs模型成功率高,可较完整的复制原始肿瘤特性,且操作安全、简便。
[Abstract]:Background & objective: most of the current models of colorectal cancer patients with xenotransplantation (patient-derived xenografts,PDXs) come from surgery, but the patients with recurrent and metastatic colorectal cancer (metastatic colorectal cancer,m CRC) have less chance of operation, so it is difficult to obtain specimens. The purpose of this study was to establish a PDXs model of m CRC by biopsy. Methods: 12 patients with colorectal cancer after radical operation had clinical symptoms and / or imaging indications of recurrence and / or metastasis, which required a definite diagnosis by puncture biopsy, and there were no contraindication for biopsy. After reserving the amount of tissue used in routine pathological diagnosis, including gene detection, the remaining specimens were used to establish the PDXs model. Results: 7 PDXs models of m CRC were successfully established, and the success rate was 77.8%. Conclusion: the PDXs model of m CRC based on puncture biopsy has a high success rate, can completely replicate the original tumor characteristics, and is safe and simple to operate.
【作者单位】：复旦大学附属肿瘤医院介入科复旦大学上海医学院肿瘤学系;
【基金】：国家重点研发计划(2016YFC0106203) 上海市科委实验动物专项(14140902202) 上海市申康新兴前沿技术项目(SHDC12014112) 上海科委自然科学基金(14ZR1407900)
【分类号】：R735.34

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