LETM2在食管鳞癌中的表达及其作用

发布时间：2018-11-26 11:38

【摘要】：目的:探讨LETM2在食管鳞癌(esophageal squamous cell carcinoma,ESCC)中的表达,研究LETM2对ESCC细胞系KYSE150和ECA109增殖和侵袭迁移的影响。方法:免疫组化(immunohistochemistry,IHC)检测90例ESCC及癌旁组织LETM2蛋白表达差异,RT-PCR和Western blot检测ESCC细胞系LETM2表达情况,慢病毒敲低KYSE150和ECA109细胞中LETM2表达。MTT和克隆形成技术检测LETM2对ESCC细胞增殖和克隆形成能力的影响,流式细胞术检测细胞周期变化,Transwell法分析LETM2敲低后细胞迁移侵袭能力的差异。结果:在ESCC组织中LETM2的表达显著高于癌旁组织,LETM2通过抑制ESCC细胞G1期向S期转化进而抑制细胞增殖,但对侵袭和迁移能力影响不大。结论:LETM2可能作为驱动基因促进ESCC的发生发展,是ESCC的关键遗传变异,可能作为ESCC早诊早治的分子标志物。
[Abstract]:Aim: to investigate the expression of LETM2 in esophageal squamous cell carcinoma (esophageal squamous cell carcinoma,ESCC) and the effect of LETM2 on the proliferation, invasion and migration of ESCC cell line KYSE150 and ECA109. Methods: the expression of LETM2 protein in 90 cases of ESCC and adjacent tissues was detected by immunohistochemistry (immunohistochemistry,IHC), and the expression of LETM2 in ESCC cell line was detected by RT-PCR and Western blot. The effects of LETM2 on the proliferation and clone formation of ESCC cells were examined by MTT and clone forming technique. The cell cycle changes were detected by flow cytometry. The difference of cell migration and invasion ability after LETM2 knockout was analyzed by Transwell assay. Results: the expression of LETM2 in ESCC was significantly higher than that in paracancerous tissues. LETM2 inhibited the proliferation of ESCC cells by inhibiting the transformation from G1 phase to S phase, but had little effect on the ability of invasion and migration. Conclusion: LETM2 may be a driving gene to promote the occurrence and development of ESCC, is the key genetic variation of ESCC, and may be used as a molecular marker for early diagnosis and early treatment of ESCC.
【作者单位】：山西医科大学基础医学院药理教研室;转化医学研究中心细胞生理学省部共建教育部重点实验室;山西省肿瘤医院;山西医科大学第一医院病理科;山西医科大学第一医院肿瘤内科;
【基金】：国家自然科学基金项目(编号:81502135) 山西医科大学博士启动基金(编号:03201006);山西医科大学青年基金(编号:057487)资助
【分类号】：R735.1

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