血清miR-17-92簇在HPV阳性宫颈癌中的早期诊断价值
发布时间：2018-11-27 16:18
【摘要】：目的检测miR-17-92簇成员分子(miR-17、miR-18a、miR-19a、miR-19b、miR-20a和miR-92a)在人乳头瘤病毒(HPV)阳性宫颈癌(CC)及上皮内瘤变(CIN)患者血清中的表达水平,并评估其对HPV阳性宫颈癌的早期诊断价值。方法收集HPV阳性宫颈癌患者150例(CC组)、HPV阳性CIN1~3级患者100例(CIN组)及单纯HPV阳性或子宫良性病变者150例(对照组)血清,采用实时定量PCR(RT-q PCR)法检测血清中miR-17-92簇成员分子的表达,结合液基薄层细胞学检测(TCT)结果,评价miR-17-92簇成员分子在HPV阳性宫颈癌中的早期诊断价值。结果与对照组相比,miR-18a和miR-92a在CC组血清中异常高表达,且其表达水平显著高于CIN组及对照组,两者在CIN3级患者中的表达水平及miR-18a在CIN1-2级患者中的表达水平均显著高于对照组(P均0.05)。受试者工作曲线(ROC曲线)分析结果显示,血清miR-18a诊断CIN3及CC(CIN3+)的曲线下面积(AUC)为0.792(95%CI:0.749~0.862),灵敏度为87%,特异度为65%;血清miR-92a诊断CIN3+的AUC为0.799(95%CI:0.744~0.847),灵敏度为55.8%,特异度为92%;TCT诊断CIN3+的AUC为0.645(95%CI:0.582~0.704),灵敏度为51.9%,特异度为77%。血清miR-18a和miR-92对宫颈癌和CIN3+的诊断效能均显著优于TCT检测(P均0.01)。结论 miR-17-92簇成员分子miR-92a和miR-18a在HPV阳性宫颈癌患者血清中高表达,并可作为HPV阳性宫颈癌的早期诊断及鉴别高级别CIN的潜在标志物。
[Abstract]:Objective to detect the expression of miR-17-92 cluster members (miR-17,miR-18a,miR-19a,miR-19b,miR-20a and miR-92a) in the serum of patients with (HPV) positive cervical cancer (CC) and (CIN). To evaluate its value in early diagnosis of HPV positive cervical cancer. Methods Serum samples were collected from 150 patients with HPV positive cervical cancer (CC group, 100 cases of), HPV positive CIN1~3 grade patients (CIN group) and 150 cases of simple HPV positive or uterine benign lesions (control group). The expression of miR-17-92 cluster molecules in serum was detected by real-time quantitative PCR (RT-q PCR) method, and the results of (TCT) were detected by liquid-based thin layer cytology. To evaluate the value of miR-17-92 cluster molecules in early diagnosis of HPV positive cervical cancer. Results compared with the control group, the expression of miR-18a and miR-92a in serum of CC group was significantly higher than that of CIN group and control group. The expression levels of miR-18a and miR-18a in CIN3 patients and CIN1-2 patients were significantly higher than those in the control group (P < 0. 05). The results of ROC curve analysis showed that the area under the curve of serum miR-18a for the diagnosis of CIN3 and CC (CIN3) was 0.792 (95%CI:0.749~0.862), the sensitivity was 87, and the specificity was 65. The AUC of serum miR-92a for the diagnosis of CIN3 was 0.799 (95%CI:0.744~0.847), the sensitivity was 55.8 and the specificity was 92; The AUC of TCT for diagnosing CIN3 was 0.645 (95%CI:0.582~0.704), the sensitivity was 51.9 and the specificity was 77. The diagnostic efficacy of serum miR-18a and miR-92 in the diagnosis of cervical cancer and CIN3 was significantly better than that of TCT (P 0.01). Conclusion miR-92a and miR-18a are highly expressed in the serum of patients with HPV positive cervical cancer and can be used as a potential marker for early diagnosis and differential diagnosis of high grade CIN in patients with HPV positive cervical cancer.
【作者单位】： 山东大学齐鲁医院妇产科;平原县第一人民医院麻醉科;
【基金】：国家自然科学基金(81372808) 山东大学基本科研业务费(2082015QLMS44)
【分类号】：R737.33
，
本文编号：2361420
[Abstract]:Objective to detect the expression of miR-17-92 cluster members (miR-17,miR-18a,miR-19a,miR-19b,miR-20a and miR-92a) in the serum of patients with (HPV) positive cervical cancer (CC) and (CIN). To evaluate its value in early diagnosis of HPV positive cervical cancer. Methods Serum samples were collected from 150 patients with HPV positive cervical cancer (CC group, 100 cases of), HPV positive CIN1~3 grade patients (CIN group) and 150 cases of simple HPV positive or uterine benign lesions (control group). The expression of miR-17-92 cluster molecules in serum was detected by real-time quantitative PCR (RT-q PCR) method, and the results of (TCT) were detected by liquid-based thin layer cytology. To evaluate the value of miR-17-92 cluster molecules in early diagnosis of HPV positive cervical cancer. Results compared with the control group, the expression of miR-18a and miR-92a in serum of CC group was significantly higher than that of CIN group and control group. The expression levels of miR-18a and miR-18a in CIN3 patients and CIN1-2 patients were significantly higher than those in the control group (P < 0. 05). The results of ROC curve analysis showed that the area under the curve of serum miR-18a for the diagnosis of CIN3 and CC (CIN3) was 0.792 (95%CI:0.749~0.862), the sensitivity was 87, and the specificity was 65. The AUC of serum miR-92a for the diagnosis of CIN3 was 0.799 (95%CI:0.744~0.847), the sensitivity was 55.8 and the specificity was 92; The AUC of TCT for diagnosing CIN3 was 0.645 (95%CI:0.582~0.704), the sensitivity was 51.9 and the specificity was 77. The diagnostic efficacy of serum miR-18a and miR-92 in the diagnosis of cervical cancer and CIN3 was significantly better than that of TCT (P 0.01). Conclusion miR-92a and miR-18a are highly expressed in the serum of patients with HPV positive cervical cancer and can be used as a potential marker for early diagnosis and differential diagnosis of high grade CIN in patients with HPV positive cervical cancer.
【作者单位】： 山东大学齐鲁医院妇产科;平原县第一人民医院麻醉科;
【基金】：国家自然科学基金(81372808) 山东大学基本科研业务费(2082015QLMS44)
【分类号】：R737.33
，
本文编号：2361420
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