miR-128调控AK2蛋白的表达通过STAT3信号通路对宫颈癌细胞生物学行为的影响
发布时间：2018-12-10 21:55
【摘要】：目的:探讨miR-128调控AK2蛋白的表达通过STAT3信号通路对宫颈癌细胞生物学行为的影响其机制。方法:q PCR和Western blot检测宫颈癌组织和细胞中AK2和miR-128的表达情况;双荧光素酶实验检测miR-128和AK2之间的相互作用;CCK-8增殖试验检测miR-128对宫颈癌细胞增值能力的影响;裸鼠体内成瘤试验检测miR-128对宫颈癌细胞成瘤能力的影响;Western blot检测miR-128对STAT3信号通路蛋白水平的影响;Western blot检测过表达AK2蛋白逆转miR-128对p-STAT3的抑制水平。结果:在宫颈癌组织中AK2表达水平相比正常宫颈组织中高,miR-128在宫颈癌C33a细胞株中表达水平较低;双荧光素酶试验证实miR-128可以直接靶向调控AK2的表达;CCK-8增殖实验表明miR-128可以抑制宫颈癌细胞株的增殖能力;体内成瘤实验表明miR-128的增高可以抑制宫颈癌细胞成瘤能力[体积(3.05±0.35)cm3vs(0.86±0.11)cm3,P=0.031;(3.26±0.39)g vs(0.89±0.15)g,P=0.016];Western blot实验表明miR-128可以抑制p-STAT的激活[(42.12±6.28)%vs(91.25±9.29)%,P0.05],而AK2的过表达又可以逆转miR-128对p-STAT的抑制作用。结论:miR-128靶向调控AK2的表达进而通过STAT3通路的激活调控宫颈癌细胞的生物学行为。
[Abstract]:Aim: to investigate the effect of miR-128 on the biological behavior of cervical cancer cells through STAT3 signaling pathway. Methods: q PCR and Western blot were used to detect the expression of AK2 and miR-128 in cervical cancer tissues and cells, and double luciferase assay was used to detect the interaction between miR-128 and AK2. CCK-8 proliferation test was used to detect the effect of miR-128 on the proliferation of cervical cancer cells and in vivo tumorigenesis test in nude mice to detect the effect of miR-128 on the tumorigenic ability of cervical cancer cells.; Western blot was used to detect the effect of miR-128 on the level of STAT3 signal pathway protein. Overexpression of AK2 protein was detected by Western blot to reverse the inhibition of p-STAT3 by miR-128. Results: the expression of AK2 in cervical carcinoma was higher than that in normal cervix, and the expression of miR-128 was lower in cervical cancer C33a cell line, and double luciferase assay showed that miR-128 could directly target the expression of AK2. CCK-8 proliferation assay showed that miR-128 could inhibit the proliferation of cervical cancer cell line. In vivo tumorigenesis experiment showed that the increase of miR-128 could inhibit the tumorigenic ability of cervical cancer cells [volume (3.05 卤0.35) cm3vs (0.86 卤0.11) cm3,P=0.031; (3.26 卤0.39) g vs (0.89 卤0.15) g P0. 016]; Western blot assay showed that miR-128 could inhibit the activation of p-STAT [(42.12 卤6.28)% vs (91.25 卤9.29)%, P0.05], while the overexpression of AK2 could reverse the inhibitory effect of miR-128 on p-STAT. Conclusion: miR-128 can regulate the expression of AK2 and regulate the biological behavior of cervical cancer cells through the activation of STAT3 pathway.
【作者单位】： 许昌学院;
【基金】：河南省医学科技攻关计划项目(201203068)
【分类号】：R737.33
[Abstract]:Aim: to investigate the effect of miR-128 on the biological behavior of cervical cancer cells through STAT3 signaling pathway. Methods: q PCR and Western blot were used to detect the expression of AK2 and miR-128 in cervical cancer tissues and cells, and double luciferase assay was used to detect the interaction between miR-128 and AK2. CCK-8 proliferation test was used to detect the effect of miR-128 on the proliferation of cervical cancer cells and in vivo tumorigenesis test in nude mice to detect the effect of miR-128 on the tumorigenic ability of cervical cancer cells.; Western blot was used to detect the effect of miR-128 on the level of STAT3 signal pathway protein. Overexpression of AK2 protein was detected by Western blot to reverse the inhibition of p-STAT3 by miR-128. Results: the expression of AK2 in cervical carcinoma was higher than that in normal cervix, and the expression of miR-128 was lower in cervical cancer C33a cell line, and double luciferase assay showed that miR-128 could directly target the expression of AK2. CCK-8 proliferation assay showed that miR-128 could inhibit the proliferation of cervical cancer cell line. In vivo tumorigenesis experiment showed that the increase of miR-128 could inhibit the tumorigenic ability of cervical cancer cells [volume (3.05 卤0.35) cm3vs (0.86 卤0.11) cm3,P=0.031; (3.26 卤0.39) g vs (0.89 卤0.15) g P0. 016]; Western blot assay showed that miR-128 could inhibit the activation of p-STAT [(42.12 卤6.28)% vs (91.25 卤9.29)%, P0.05], while the overexpression of AK2 could reverse the inhibitory effect of miR-128 on p-STAT. Conclusion: miR-128 can regulate the expression of AK2 and regulate the biological behavior of cervical cancer cells through the activation of STAT3 pathway.
【作者单位】： 许昌学院;
【基金】：河南省医学科技攻关计划项目(201203068)
【分类号】：R737.33
【相似文献】
相关期刊论文 前10条
1 刘辉;英国推广液态宫颈癌细胞学检查[J];中华医学信息导报;2004年01期
2 卢美松;邓锁;王泽华;;体外模拟二氧化碳人工气腹对宫颈癌细胞生长的影响[J];中国实用妇科与产科杂志;2007年01期
3 周晨慧;;干扰素-γ对宫颈癌细胞免疫分子表达的影响[J];现代医药卫生;2007年01期
4 潘惠艳;赵群;詹阳;赵丽红;张卫华;吴玉梅;;电压门控钠离子通道表达对宫颈癌细胞增殖侵袭转移作用的研究[J];中国肿瘤临床;2012年04期
5 郭鸣，崔正言，，赵温利，温培娥，崔树龄;宫颈癌细胞细胞核DNA含量及形态的定量分析[J];中国肿瘤临床;1996年10期
6 俞帆,陈钢,欧阳明,田敬学,李萍;分形理论在宫颈癌细胞核形态研究中的应用[J];云南大学学报(自然科学版);1998年S2期
7 郑秀惠,陈竹钦,李力,梁培禾;~(252)锎中子放疗对宫颈癌细胞凋亡和增殖的影响[J];重庆医学;2005年06期
8 王海燕;钱伟丽;余海忠;林淦;;宫颈癌细胞中调控基因的研究进展[J];广东医学;2011年23期
9 潘s
本文编号：2371264
本文链接：https://www.wllwen.com/yixuelunwen/zlx/2371264.html
