SBEM与Ki67在三阴乳腺癌组织中的表达对三阴乳腺癌患者的联合预测意义

发布时间：2018-12-22 07:57

【摘要】：目的:探究SBEM与Ki67在三阴乳腺癌组织中的表达情况与三阴乳腺癌患者临床病理特征之间的关系,探究联合检测SBEM与Ki67在三阴乳腺癌组织中的表达对三阴乳腺癌患者预后之间的关系。材料与方法:本研究选取来自于沈阳军区总医院全军肿瘤诊治中心收治的,时间由2005年9月至2011年3月的女性三阴乳腺癌术后患者共97例。选取病理资料来自于沈阳军区总医院病理科封存的术后组织蜡块,应用PV9000两步法对三阴乳腺癌患者进行乳腺上皮粘蛋白表达情况的检测,查阅临床相关病例资料获取患者的Ki67表达情况以及年龄、肿瘤大小、肿瘤分期、病理类型、淋巴结转移情况、复发转移情况、BMI指数、脉管癌栓等临床病理特征,并对5名患者的Ki67表达进行重新染色抽查。对患者进行中位时间为49个月的随访,获取患者的生存及复发转移状况。使用SPSS21.0进行统计学分析获取结果。各亚组间的计量资料比较均采用Pearson’sχ2检验;对患者的生存分析采用K-M法。采用Log-rank检验对组间的生存率曲线进行比较。所有检验方法为双侧检验,P0.05表示差异具有统计学意义。结果:1.SBEM高表达率达57.7%(56/97),低表达或不表达占42.3%(41/97)。Ki67的高表达率可达60.8%(59/97),低表达占39.2%(38/97)。SBEM与Ki67高表达情况不具有相关型(p0.05)。两者同时高表达占总患者数的37.1%(36/97)。2.SBEM蛋白的表达情况与淋巴结转移、有无复发转移相关,与年龄、临床分期、肿瘤大小、病理类型、脉管瘤栓及BMI指数无关。3.Ki67表达情况与肿瘤大小、临床分期、复发转移相关;与年龄、淋巴结转移情况、病理类型、脉管癌栓、BMI指数无关。4.SBEM与Ki67同时高表达的患者的5年总生存率最差(52.7%,19/36),5年生存率与SBEM-Ki67-组相比具有统计学差异(P=0.020)。5.与各亚组的五年总生存率类似,SBEM与Ki67同时高表达的患者的5年无进展生存率最差(58.3%,19/36),5年无进展生存率与SBEM-Ki67-组相比具有统计学差异(P=0.023)。结论:1.在三阴乳腺癌组织中,SBEM的与淋巴结转移、有无复发转移相关,与年龄、临床分期、肿瘤大小、病理类型、脉管瘤栓及BMI指数无关。2.在三阴乳腺癌组织中,Ki67表达情况与肿瘤大小、临床分期、复发转移相关;与年龄、淋巴结转移情况、病理类型、脉管癌栓、BMI指数无关。3.SBEM与Ki67均高表达提示患者的总生存期和无进展生存期低于二者皆低表达的患者。4.联合检测SBEM与Ki67在三阴乳腺癌组织中的表达情况,对三阴乳腺癌患者的生存预测和后续治疗可能有较大的意义。
[Abstract]:Objective: to investigate the relationship between the expression of SBEM and Ki67 and the clinicopathological features of Sanyin breast cancer. To explore the relationship between the expression of SBEM and Ki67 in breast cancer tissues and prognosis of patients with triple negative breast cancer. Materials and methods: a total of 97 female patients with triple yin breast cancer from September 2005 to March 2011 were selected and treated in the Army tumor diagnosis and treatment Center of Shenyang military region General Hospital from September 2005 to March 2011. The pathological data were collected from the tissue wax blocks of the Department of Pathology of Shenyang military region General Hospital, and the expression of mucin in breast epithelium was detected by PV9000 two-step method. The expression of Ki67, age, tumor size, tumor stage, pathological type, lymph node metastasis, recurrence and metastasis, BMI index, vascular tumor thrombus and other clinicopathological features were obtained by consulting clinical data of related cases. The expression of Ki67 in 5 patients was examined by re-staining. Patients were followed up for 49 months for survival, recurrence and metastasis. SPSS21.0 was used for statistical analysis to obtain the results. Pearson's 蠂 2 test was used to compare the metrological data among the subgroups, and K-M method was used to analyze the survival of the patients. Log-rank test was used to compare the survival curves between groups. All the test methods were bilateral test, P0.05 indicated that the difference was statistically significant. Results: the high expression rate of 1.SBEM was 57.7% (56 / 97), low expression or non-expression was 42.3% (41 / 97). The high expression rate of Ki67 was 60.8% (59 / 97). The low expression rate was 39.2% (38 / 97). SBEM was not correlated with the high expression of Ki67 (p0.05). The expression of 2.SBEM protein was correlated with lymph node metastasis, recurrence and metastasis, and was correlated with age, clinical stage, tumor size and pathological type. The expression of 3.Ki67 was correlated with tumor size, clinical stage, recurrence and metastasis. There was no correlation between age, lymph node metastasis, pathological type, vascular tumor thrombus, BMI index. The 5-year overall survival rate of patients with high expression of 4.SBEM and Ki67 was the worst (52.7% / 36). The 5-year survival rate was significantly higher than that in SBEM-Ki67- group (P0. 020). The 5-year overall survival rate of patients with high expression of SBEM and Ki67 was the worst (58.3 / 19 / 36), and the 5-year progression-free survival rate was statistically different from that of SBEM-Ki67- group (P0. 023). Conclusion: 1. SBEM was associated with lymph node metastasis, recurrence and metastasis, but not with age, clinical stage, tumor size, pathological type, vascular thrombus and BMI index. The expression of Ki67 was correlated with tumor size, clinical stage, recurrence and metastasis. There was no correlation between age, lymph node metastasis, pathological type, vascular tumor thrombus, BMI index. The high expression of 3.SBEM and Ki67 showed that the total survival and progression free survival of patients were lower than those of patients with low expression of both. 4. Combined detection of the expression of SBEM and Ki67 in the tissues of Sanyin breast cancer may be of great significance for the survival prediction and follow-up treatment of patients with triple negative breast cancer.
【学位授予单位】：辽宁中医药大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R737.9

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