肺腺癌中MMP-9、MMP-13、HIF-1α表达与临床病理特征、EGFR突变及预后的关系

发布时间：2019-01-05 13:03

【摘要】：目的探讨MMP-9、MMP-13、HIF-1α在肺腺癌中的表达,分析其与肺腺癌临床病理特征、EGFR突变及预后的关系。方法采用免疫组化SP法检测629例肺腺癌组织中MMP-9、MMP-13、HIF-1α的表达,并选取癌旁正常组织、肺炎性假瘤样增生各50例作为对照。采用实时荧光PCR法检测629例肺腺癌EGFR的突变,并对患者进行随访。结果肺腺癌组中MMP-9、MMP-13、HIF-1α的表达均高于对照组(P0.001)。MMP-9表达与患者淋巴结转移及肿瘤大小有关(P0.05);MMP-13表达与患者吸烟史、TNM分期、淋巴结转移及肿瘤大小有关(P0.05);HIF-1α表达与患者吸烟史、淋巴结转移有关(P0.05);但三者表达均与EGFR突变无关(P0.05)。经Kaplan-Meier生存分析结果显示:MMP-9、MMP-13和HIF-1α阳性组12、24、36个月的累积生存率均明显低于阴性组;COX多因素生存分析结果显示:EGFR突变、MMP-9、MMP-13、HIF-1α的表达、肿瘤大小、TNM分期均是肺腺癌患者生存状况的独立危险因素(P0.05)。结论肺腺癌组织中MMP-9、MMP-13、HIF-1α存在过表达,其表达均与患者淋巴结转移有关,但与EGFR突变无关。MMP-9、MMP-13、HIF-1α呈阳性、EGFR无突变患者生存率低,且均为肺腺癌患者生存状况的独立危险因素。
[Abstract]:Objective to investigate the expression of MMP-9,MMP-13,HIF-1 伪 in lung adenocarcinoma and its relationship with clinicopathological features, EGFR mutation and prognosis. Methods Immunohistochemical SP method was used to detect the expression of MMP-9,MMP-13,HIF-1 伪 in 629 cases of lung adenocarcinoma, and 50 cases of pulmonary pseudotumor-like hyperplasia and 50 cases of pulmonary pseudotumor-like hyperplasia were selected as control. The mutation of EGFR in 629 cases of lung adenocarcinoma was detected by real-time fluorescence PCR and followed-up. Results the expression of MMP-9,MMP-13,HIF-1 伪 in lung adenocarcinoma group was higher than that in control group (P0. 001). The expression of MMP-9 was related to lymph node metastasis and tumor size (P0.05). MMP-13 expression was related to smoking history, TNM stage, lymph node metastasis and tumor size (P0.05); HIF-1 伪 expression was related to smoking history and lymph node metastasis (P0.05); but the expression of EGFR was not correlated with EGFR mutation (P0.05). The results of Kaplan-Meier survival analysis showed that the cumulative survival rate at 36 months in MMP-9,MMP-13 and HIF-1 伪 positive group was significantly lower than that in negative group. COX multivariate survival analysis showed that EGFR mutation, MMP-9,MMP-13,HIF-1 伪 expression, tumor size and TNM stage were independent risk factors for survival status of lung adenocarcinoma patients (P0.05). Conclusion there is overexpression of MMP-9,MMP-13,HIF-1 伪 in lung adenocarcinoma, which is related to lymph node metastasis, but not related to EGFR mutation. MMP-9,MMP-13,HIF-1 伪 is positive and the survival rate is low in patients without EGFR mutation. All of them were independent risk factors for survival status of patients with lung adenocarcinoma.
【作者单位】：河北医科大学第四医院病理科;河北医科大学第四医院放疗科;河北医科大学第四医院肿瘤研究所;
【基金】：基金项目:河北省财政厅冀财(2017043367)
【分类号】：R734.2

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