口腔菌群结构动态变化与胃癌发生发展相关性分析

发布时间：2019-01-08 16:41

【摘要】：目的探讨胃癌患者口腔菌群结构动态变化与胃癌的相关性。方法采用微生物鉴定芯片技术高通量分析和比较胃癌患者和健康对照受试者唾液中菌群结构动态变化与胃癌发生发展相关性;运用SPSS软件对基因芯片中424种微生物探针信号进行分析;采用dChip软件分析口腔菌群差异,采用MEGA软件制作系统进化树进行多样性分析。结果与对照组比较,胃癌组患者的唾液中有26种菌群显著减少,差异有统计学意义。其中包括拟杆菌门(Bacteroidetes)5种、变形菌门(Proteobacteria)8种、放线菌门(Actinobacteria)1种、厚壁菌门(Firmicutes)9种、梭杆菌门(Fusobacteria)3种。结论胃癌患者口腔菌群发生变化,有显著变化的细菌种类有望做为胃癌检测的生物标志物。
[Abstract]:Objective to investigate the correlation between the dynamic changes of oral flora and gastric cancer in patients with gastric cancer. Methods the microbiological identification microarray technique was used to analyze and compare the relationship between the dynamic changes of salivary flora structure and the development of gastric cancer in patients with gastric cancer and healthy control subjects. SPSS software was used to analyze the signal of 424 kinds of microbe probe in gene chip, dChip software was used to analyze the difference of oral flora, and MEGA software was used to make phylogenetic tree for diversity analysis. Results compared with the control group, there were 26 kinds of microflora in saliva of gastric cancer patients, and the difference was statistically significant. There were 5 species of (Bacteroidetes), 8 species of (Proteobacteria), 1 species of (Actinobacteria), 9 species of (Firmicutes) and 3 species of (Fusobacteria). Conclusion the oral microflora changes in gastric cancer patients, and the bacteria species with significant changes may be used as biomarkers for gastric cancer detection.
【作者单位】：山东师范大学生命科学学院;齐鲁师范学院生命科学学院;济宁医学院行为医学教育研究所;山东省千佛山医院;山东省食品药品检验研究院;
【基金】：山东省自然科学基金项目(No.ZR2015CL005)
【分类号】：R735.2

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