双氢青蒿素对肺癌细胞增殖的抑制作用

发布时间：2019-01-21 08:50

【摘要】：目的探讨双氢青蒿素对肺癌细胞生长增殖的抑制作用。方法用MTT法观察浓度为4、8、16、32、64、128、256μmol/L的双氢青蒿素对三种不同的肺癌细胞SPC-A-1、Calua-3、PC-14作用48 h的增殖抑制作用,同样用MTT检测29μmol/L的双氢青蒿素作用时间为12、24、48、72、96 h后的肺癌细胞增殖情况,经DNA ladder和流式细胞仪检测细胞凋亡情况。结果双氢青蒿素对三种肺癌细胞的抑制率从大到小依次为:SPC-A-1、PC-14、Calua-3。肺癌细胞SPC-A-1在双氢青蒿素的作用下细胞抑制率较PC-14、Calua-3肺癌细胞大,利用软件计算出细胞SPC-A-1、PC-14、Calua-3的IC50分别为28.9、46.9、78.6μmol/L。双氢青蒿素作用时间为12、24、48、72和96 h的细胞抑制率较0 h差异显著(P0.01)。说明随着作用时间的加长,抑制增殖效果越强。29μmol/L的双氢青蒿素作用48 h后的肺癌细胞出现明显的类似梯子样条带,说明双氢青蒿素可以促进肺癌细胞的增殖。流式细胞仪结果表明,经药物作用后的SPC-A-1细胞的凋亡率较对照组显著增高(P0.01),这表明双氢青蒿素抑制肺癌细胞SPC-A-1的增殖主要是通过诱导细胞凋亡所引起的。结论双氢青蒿素可通过诱导细胞凋亡抑制肺癌细胞增殖分化。
[Abstract]:Objective to investigate the inhibitory effect of dihydroartemisinin on the growth and proliferation of lung cancer cells. Methods MTT assay was used to observe the inhibitory effect of dihydroartemisinin on the proliferation of three different lung cancer cells treated with SPC-A-1,Calua-3,PC-14 for 48 h. MTT was also used to detect the proliferation of lung cancer cells with 29 渭 mol/L dihydroartemisinin for 12: 2424, 48, 722, 96 h, and apoptosis was detected by DNA ladder and flow cytometry. Results the inhibitory rates of dihydroartemisinin on three kinds of lung cancer cells were as follows: SPC-A-1,PC-14,Calua-3. The inhibition rate of SPC-A-1 in lung cancer cell line was higher than that in PC-14,Calua-3 lung cancer cell line. The IC50 of cell SPC-A-1,PC-14,Calua-3 calculated by software was 28.9n46.9 渭 mol/L. and 78.6 渭 mol/L., respectively. The inhibitory rate of dihydroartemisinin was significantly higher than that of 0 h (P0.01). The results showed that with the increase of the time of treatment, the inhibition of proliferation was stronger. After 48 h of treatment with 29 渭 mol/L dihydroartemisinin, there were obvious ladder spline bands in lung cancer cells, indicating that dihydroartemisinin could promote the proliferation of lung cancer cells. The results of flow cytometry showed that the apoptosis rate of SPC-A-1 cells treated with drugs was significantly higher than that of the control group (P0.01). This suggests that dihydroartemisinin inhibits the proliferation of lung cancer cell line SPC-A-1 mainly by inducing apoptosis. Conclusion Dihydroartemisinin can inhibit the proliferation and differentiation of lung cancer cells by inducing apoptosis.
【作者单位】：武汉市中医医院综合药学部;武汉市中医医院肿瘤科;
【基金】：湖北省自然科学基金资助(No.2012FKB04450)
【分类号】：R734.2

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