MicroRNA-150在结膜黏膜相关淋巴组织淋巴瘤增殖、迁移及侵袭中的作用
发布时间：2019-01-24 20:48
【摘要】：目的观察结膜黏膜相关淋巴组织(MALT)淋巴瘤中microRNA-150(miR-150)的表达,探讨miR-150在结膜MALT淋巴瘤中影响肿瘤细胞增殖、迁移与侵袭的机制。方法使用qPCR法检测第二军医大学长征医院收治的3例结膜MALT淋巴瘤患者肿瘤组织及瘤旁组织中miR-150及其可能的下游靶分子Cbl-b的表达。将miR-150抑制物和阴性对照转染人多发性骨髓瘤细胞株RPMI 8226,采用CCK-8法和流式细胞术研究miR-150对RPMI 8226细胞增殖和凋亡的影响,通过Transwell实验研究miR-150对RPMI 8226细胞迁移和侵袭的影响,用蛋白质印迹法检测miR-150对RPMI 8226细胞中Cbl-b表达的调控。结果与瘤旁组织相比,结膜MALT淋巴瘤组织中miR-150表达上调(P0.05,P0.01);抑制miR-150后,RPMI 8226细胞的增殖受到抑制,细胞凋亡明显增加,迁移和侵袭能力降低,与阴性对照组相比差异有统计学意义(P0.05,P0.01)。在结膜MALT淋巴瘤组织中,miR-150下游靶基因Cbl-b表达下调(P0.01);抑制miR-150后,RPMI 8226细胞内Cbl-b蛋白的表达上调(P0.01)。结论 MiR-150对淋巴瘤细胞的增殖、迁移和侵袭有促进作用,其表达上调参与了MALT淋巴瘤的发生。其机制可能与miR-150对下游分子Cbl-b的抑制性调控有关。
[Abstract]:Objective to observe the expression of microRNA-150 (miR-150) in conjunctival mucosa-associated lymphoid tissue (MALT) lymphoma and to explore the mechanism of miR-150 affecting the proliferation, migration and invasion of tumor cells in conjunctival MALT lymphoma. Methods qPCR method was used to detect the expression of miR-150 and its possible downstream target Cbl-b in tumor tissues and adjacent tissues of 3 patients with conjunctival MALT lymphoma treated in Changzheng Hospital of second military Medical University. Human multiple myeloma cell line RPMI 8226 was transfected with miR-150 inhibitor and negative control. The effect of miR-150 on proliferation and apoptosis of RPMI 8226 cells was studied by CCK-8 assay and flow cytometry. The effect of miR-150 on the migration and invasion of RPMI 8226 cells was studied by Transwell assay. The regulation of Cbl-b expression in RPMI 8226 cells by miR-150 was detected by Western blotting. Results the expression of miR-150 was up-regulated in conjunctival MALT lymphoma (P0.05, P0.01). After inhibiting miR-150, the proliferation of RPMI 8226 cells was inhibited, the apoptosis of RPMI 8226 cells was significantly increased, the migration and invasion ability was decreased, compared with the negative control group, the difference was statistically significant (P0.05, P0.01). In conjunctival MALT lymphoma, the Cbl-b expression of miR-150 downstream target gene was down-regulated (P0.01), and the expression of Cbl-b protein was up-regulated in RPMI 8226 cells after miR-150 inhibition (P0.01). Conclusion MiR-150 can promote the proliferation, migration and invasion of Lymphoma cells, and its up-regulation may be involved in the pathogenesis of MALT lymphoma. The mechanism may be related to the inhibitory regulation of Cbl-b by miR-150.
【作者单位】： 第二军医大学长征医院眼科;
【基金】：上海市自然科学基金(14ZR1414000) 上海市卫生和计划生育委员会科研课题面上项目(201445)~~
【分类号】：R739.7
本文编号：2414843
[Abstract]:Objective to observe the expression of microRNA-150 (miR-150) in conjunctival mucosa-associated lymphoid tissue (MALT) lymphoma and to explore the mechanism of miR-150 affecting the proliferation, migration and invasion of tumor cells in conjunctival MALT lymphoma. Methods qPCR method was used to detect the expression of miR-150 and its possible downstream target Cbl-b in tumor tissues and adjacent tissues of 3 patients with conjunctival MALT lymphoma treated in Changzheng Hospital of second military Medical University. Human multiple myeloma cell line RPMI 8226 was transfected with miR-150 inhibitor and negative control. The effect of miR-150 on proliferation and apoptosis of RPMI 8226 cells was studied by CCK-8 assay and flow cytometry. The effect of miR-150 on the migration and invasion of RPMI 8226 cells was studied by Transwell assay. The regulation of Cbl-b expression in RPMI 8226 cells by miR-150 was detected by Western blotting. Results the expression of miR-150 was up-regulated in conjunctival MALT lymphoma (P0.05, P0.01). After inhibiting miR-150, the proliferation of RPMI 8226 cells was inhibited, the apoptosis of RPMI 8226 cells was significantly increased, the migration and invasion ability was decreased, compared with the negative control group, the difference was statistically significant (P0.05, P0.01). In conjunctival MALT lymphoma, the Cbl-b expression of miR-150 downstream target gene was down-regulated (P0.01), and the expression of Cbl-b protein was up-regulated in RPMI 8226 cells after miR-150 inhibition (P0.01). Conclusion MiR-150 can promote the proliferation, migration and invasion of Lymphoma cells, and its up-regulation may be involved in the pathogenesis of MALT lymphoma. The mechanism may be related to the inhibitory regulation of Cbl-b by miR-150.
【作者单位】： 第二军医大学长征医院眼科;
【基金】：上海市自然科学基金(14ZR1414000) 上海市卫生和计划生育委员会科研课题面上项目(201445)~~
【分类号】：R739.7
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1 蔡星;分泌的microRNA-214和microRNA-150在肿瘤与免疫系统相互作用中的功能与机制研究[D];南京大学;2012年
，本文编号：2414843
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