卵巢癌中gC1qR的表达及其凋亡机制

发布时间：2019-01-27 17:47

【摘要】：目的探讨球状C1q受体(g C1qR)在卵巢癌中的表达,并分析其潜在的分子机制。方法收集48例卵巢癌组织和卵巢癌细胞株SKOV3为分析对象,采用PCR、Western blot分别检测g C1qR mRNA及蛋白质的表达;采用MTT比色法、Transwel实验及流式细胞技术检测卵巢癌细胞的增殖、迁移及凋亡情况;以分子荧光探针H2DCFDA、Fluo-3/AM、JC-1分别测定细胞内活性氧(ROS)、Ca~(2+)浓度以及线粒体膜电位,评估卵巢癌细胞线粒体功能的变化。结果卵巢癌组织中g C1qR mRNA及蛋白质表达分别为2.61±0.34、0.20±0.02,均明显低于其相应的癌旁正常卵巢组织7.32±1.25和0.67±0.06,差异有显著性(P0.001)。g C1qR基因过表达,可显著降低卵巢癌细胞增殖活性、迁移率;其细胞凋亡率明显增加,且呈现显著的细胞凋亡特征;同时,卵巢癌细胞线粒体中ROS含量、细胞内Ca~(2+)浓度显著增高,线粒体膜电位显著减少。结论 g C1qR基因在卵巢癌细胞凋亡中发挥重要作用,可通过引发线粒体功能障碍诱导卵巢癌细胞凋亡。
[Abstract]:Objective to investigate the expression of globular C1q receptor (g C1qR) in ovarian cancer and its potential molecular mechanism. Methods 48 cases of ovarian cancer tissue and ovarian cancer cell line SKOV3 were collected for analysis. PCR,Western blot was used to detect the expression of g C1qR mRNA and protein respectively. The proliferation, migration and apoptosis of ovarian cancer cells were detected by MTT colorimetry, Transwel assay and flow cytometry. The concentration of reactive oxygen species (ROS), Ca~ _ (2) and mitochondrial membrane potential (MMP) in ovarian cancer cells were measured with the molecular fluorescence probe H2DCFDAA Fluo-3 / AMJC-1 to evaluate the changes of mitochondrial function in ovarian cancer cells. Results the expression of g C1qR mRNA and protein in ovarian carcinoma was 2.61 卤0.34 and 0.20 卤0.02, respectively, which was significantly lower than that in normal ovarian tissues (7.32 卤1.25,0.67 卤0.06). The difference was significant (P0. 001). G C1qR gene overexpression could significantly reduce the proliferation activity and mobility of ovarian cancer cells. At the same time, the content of ROS in mitochondria and the concentration of Ca~ (2) in the mitochondria of ovarian cancer cells increased significantly, and the mitochondrial membrane potential decreased significantly. Conclusion g C1qR gene plays an important role in apoptosis of ovarian cancer cells, and it can induce apoptosis of ovarian cancer cells by inducing mitochondrial dysfunction.
【作者单位】：南京医科大学附属妇产医院病理科;苏州大学附属第一医院病理科;
【基金】：江苏省自然科学基金(BK20151078)
【分类号】：R737.31

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