人胚胎干细胞对肝癌HepG2细胞体外抑制作用的研究
发布时间：2019-02-14 15:06
【摘要】：目的:研究体外共培养情况下人胚胎干细胞H9对肝癌HepG2细胞的抑制作用。方法:建立人胚胎干细胞(H9)与肝癌HepG2细胞共培养体系,显微镜下观察胚胎干细胞对肿瘤细胞生物学行为的影响,流式细胞仪检测H9细胞对肿瘤细胞的凋亡与周期的影响,Transwell小室法检测H9细胞对HepG2细胞侵袭和迁移的影响,基因芯片分析共培养后HepG2细胞全基因组的表达谱变化。结果:结果发现共培养过程中,肝癌HepG2细胞生长受到抑制,随共培养时间延长,细胞数量逐渐减少,出现老化或凋亡迹象;流式细胞术检测发现HepG2细胞凋亡率显著增加,细胞周期被阻滞于G0/G1期;Transwell实验发现HepG2细胞侵袭、迁移力均降低;基因芯片结果发现HepG2细胞全基因组表达谱发生了显著变化,差异基因涉及多条信号通路。结论:人胚胎干细胞H9体外对人肝癌细胞HepG2有一定程度的抑制作用。
[Abstract]:Aim: to study the inhibitory effect of human embryonic stem cell H 9 on HepG2 cells in vitro. Methods: the co-culture system of human embryonic stem cells (H9) and hepatocellular carcinoma (HepG2) cells was established. The effects of embryonic stem cells on the biological behavior of tumor cells were observed under microscope, and the effects of H9 cells on apoptosis and cell cycle were detected by flow cytometry. The effect of H9 cells on the invasion and migration of HepG2 cells was detected by Transwell chamber assay. The gene microarray was used to analyze the expression profile of the whole genome of HepG2 cells after co-culture. Results: the results showed that the growth of hepatoma HepG2 cells was inhibited during co-culture. With the prolongation of co-culture time, the number of hepatoma cells decreased gradually, and the cells showed signs of aging or apoptosis. Flow cytometry showed that the apoptosis rate of HepG2 cells was significantly increased, cell cycle was blocked in G0/G1 phase, and HepG2 cell invasion and migration decreased by Transwell assay. The results of gene chip showed that the whole genome expression profile of HepG2 cells changed significantly, and the differentially expressed genes were involved in many signal pathways. Conclusion: human embryonic stem cell H 9 has a certain inhibitory effect on human hepatoma cell HepG2 in vitro.
【作者单位】： 重庆医科大学分子医学与肿瘤研究中心;重庆医科大学分子医学与肿瘤研究中心重庆市生物化学与分子药理学重点实验室;
【基金】：国家自然科学基金资助项目(81071770,81201679)
【分类号】：R73-36
本文编号：2422322
[Abstract]:Aim: to study the inhibitory effect of human embryonic stem cell H 9 on HepG2 cells in vitro. Methods: the co-culture system of human embryonic stem cells (H9) and hepatocellular carcinoma (HepG2) cells was established. The effects of embryonic stem cells on the biological behavior of tumor cells were observed under microscope, and the effects of H9 cells on apoptosis and cell cycle were detected by flow cytometry. The effect of H9 cells on the invasion and migration of HepG2 cells was detected by Transwell chamber assay. The gene microarray was used to analyze the expression profile of the whole genome of HepG2 cells after co-culture. Results: the results showed that the growth of hepatoma HepG2 cells was inhibited during co-culture. With the prolongation of co-culture time, the number of hepatoma cells decreased gradually, and the cells showed signs of aging or apoptosis. Flow cytometry showed that the apoptosis rate of HepG2 cells was significantly increased, cell cycle was blocked in G0/G1 phase, and HepG2 cell invasion and migration decreased by Transwell assay. The results of gene chip showed that the whole genome expression profile of HepG2 cells changed significantly, and the differentially expressed genes were involved in many signal pathways. Conclusion: human embryonic stem cell H 9 has a certain inhibitory effect on human hepatoma cell HepG2 in vitro.
【作者单位】： 重庆医科大学分子医学与肿瘤研究中心;重庆医科大学分子医学与肿瘤研究中心重庆市生物化学与分子药理学重点实验室;
【基金】：国家自然科学基金资助项目(81071770,81201679)
【分类号】：R73-36
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