巨噬细胞加帽蛋白与胃癌细胞增殖及迁移能力的关系
发布时间：2019-02-19 19:06
【摘要】：目的:探讨巨噬细胞加帽蛋白(macrophage-capping protein,CapG)对胃癌细胞系迁移和增殖能力的影响。方法:采用real-time PCR方法检测了4种胃癌细胞AGS、BCG823、PHM82、MNK45中CapG基因的表达情况,选择低表达并且易转染的AGS细胞作为研究对象,设计针对CapG的特异性引物并合成重组质粒。构建可以表达CapG的慢病毒包装系统,通过侵染人胃癌细胞系AGS,建立可稳定表达CapG的细胞株。通过CCK8实验分析过表达CapG基因对AGS细胞的生长和增殖能力的影响,细胞划痕以及Transwell小室实验分析过表达CapG基因对AGS细胞迁移能力的影响。结果:过表达CapG后,AGS细胞的生长速度略低于对照组,但二者间差异无统计学意义(t=2.424,P=0.073)。划痕试验显示CapG实验组划痕间距相对于对照组明显缩小,两组平均缩小距离分别为336.99μm和45.54μm,差异有统计学意义(t=14.97,P=0.004)。Transwell试验显示CapG实验组和对照组穿膜细胞数目分别为176个和70个,CapG实验组显著多于对照组,差异有统计学意义(t=40.00,P0.001)。结论:过表达CapG基因对胃癌细胞系AGS细胞的生长和增殖无显著影响,过表达CapG基因能促进胃癌细胞系AGS细胞的迁移。
[Abstract]:Aim: to investigate the effect of macrophage capsin (macrophage-capping protein,CapG) on migration and proliferation of gastric cancer cell lines. Methods: the expression of CapG gene in AGS,BCG823,PHM82,MNK45 of four gastric cancer cells was detected by real-time PCR method. The low expression and easy transfection AGS cells were selected as the research objects. Specific primers for CapG were designed and recombinant plasmids were synthesized. A lentivirus packaging system expressing CapG was constructed and a stable CapG expression cell line was established by infecting human gastric cancer cell line AGS,. The effects of overexpression of CapG gene on the growth and proliferation of AGS cells were analyzed by CCK8 assay, and the effects of overexpression of CapG gene on the migration ability of AGS cells were analyzed by cell scratch and Transwell chamber experiments. Results: after overexpression of CapG, the growth rate of AGS cells was slightly lower than that of the control group, but there was no significant difference between the two groups (t = 2.424, P < 0.073). Scratch test showed that the distance between scratches in CapG group was significantly smaller than that in control group. The mean reduction distance between the two groups was 336.99 渭 m and 45.54 渭 m, respectively. The difference was statistically significant (t = 14.97, t = 14.97). P0. 004). Transwell test showed that the number of perforating cells in CapG group and control group were 176 and 70, respectively. The number of perforating cells in CapG group was significantly higher than that in control group (t0. 000. 00n P0. 001). Conclusion: overexpression of CapG gene has no significant effect on the growth and proliferation of gastric cancer cell line AGS. Overexpression of CapG gene can promote the migration of gastric cancer cell line AGS.
【作者单位】： 北京大学第三医院消化科;北京大学基础医学院病原生物学系;
【基金】：国家自然科学基金(81450024)资助~~
【分类号】：R735.2
本文编号：2426774
[Abstract]:Aim: to investigate the effect of macrophage capsin (macrophage-capping protein,CapG) on migration and proliferation of gastric cancer cell lines. Methods: the expression of CapG gene in AGS,BCG823,PHM82,MNK45 of four gastric cancer cells was detected by real-time PCR method. The low expression and easy transfection AGS cells were selected as the research objects. Specific primers for CapG were designed and recombinant plasmids were synthesized. A lentivirus packaging system expressing CapG was constructed and a stable CapG expression cell line was established by infecting human gastric cancer cell line AGS,. The effects of overexpression of CapG gene on the growth and proliferation of AGS cells were analyzed by CCK8 assay, and the effects of overexpression of CapG gene on the migration ability of AGS cells were analyzed by cell scratch and Transwell chamber experiments. Results: after overexpression of CapG, the growth rate of AGS cells was slightly lower than that of the control group, but there was no significant difference between the two groups (t = 2.424, P < 0.073). Scratch test showed that the distance between scratches in CapG group was significantly smaller than that in control group. The mean reduction distance between the two groups was 336.99 渭 m and 45.54 渭 m, respectively. The difference was statistically significant (t = 14.97, t = 14.97). P0. 004). Transwell test showed that the number of perforating cells in CapG group and control group were 176 and 70, respectively. The number of perforating cells in CapG group was significantly higher than that in control group (t0. 000. 00n P0. 001). Conclusion: overexpression of CapG gene has no significant effect on the growth and proliferation of gastric cancer cell line AGS. Overexpression of CapG gene can promote the migration of gastric cancer cell line AGS.
【作者单位】： 北京大学第三医院消化科;北京大学基础医学院病原生物学系;
【基金】：国家自然科学基金(81450024)资助~~
【分类号】：R735.2
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1 徐岳军;竺王玉;何剑营;陈冬冬;刘晓光;;巨噬细胞加帽蛋白与肺癌患者转移预后的关系[J];中国卫生检验杂志;2011年10期
，本文编号：2426774
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