骨内单次注射辛伐他汀促进小鼠乳腺癌肿瘤血管正常化的研究

发布时间：2019-02-19 21:37

【摘要】：目的研究骨内单次注射辛伐他汀对小鼠乳腺癌肿瘤血管结构和功能的影响。方法构建小鼠乳腺癌原位模型,分别给予骨内单次注射辛伐他汀(50μg)或空白载体。α-SMA/CD31免疫荧光双染观察肿瘤血管的周细胞/血管内皮细胞覆盖率;尾静脉注射伊文氏蓝染料观察肿瘤血管渗透性;免疫组化检测肿瘤内部HIF-1α表达水平的变化。结果免疫荧光双染发现与对照组相比辛伐他汀组肿瘤血管周细胞覆盖增多(P0.05);肿瘤组织内伊文氏蓝含量显著降低(P0.05),提示肿瘤血管渗透性减低,肿瘤血管趋于成熟;免疫组化显示肿瘤内部HIF-1α的表达水平降低,乳腺癌肿瘤组织缺氧改善。结论骨内单次注射辛伐他汀能够提高新生血管的周细胞覆盖,促进乳腺癌肿瘤血管正常化。
[Abstract]:Objective to study the effect of single intraosseous injection of simvastatin on vascular structure and function of breast cancer in mice. Methods the in situ model of mouse breast cancer was established by single intraosseous injection of simvastatin (50 渭 g) or blank vector. 伪-SMA/CD31 immunofluorescence double staining was used to observe the pericyte / endothelial cell coverage of tumor vessels. The vascular permeability was observed by injecting Yi Wen blue dye into the tail vein, and the expression of HIF-1 伪 in tumor was detected by immunohistochemistry. Results compared with the control group, the number of tumor perivascular cells in simvastatin group was higher than that in control group (P0.05). Yi Wen blue content in tumor tissue decreased significantly (P0.05), suggesting that tumor vascular permeability decreased, tumor blood vessels tended to mature; immunohistochemistry showed that the expression level of HIF-1 伪 in tumor decreased, breast cancer tumor tissue hypoxia improved. Conclusion single intraosseous injection of simvastatin can increase the pericyte coverage of neovascularization and promote the normalization of tumor blood vessels in breast cancer.
【作者单位】：北京大学第三医院骨科;北京大学第三医院肿瘤化疗与放射病科;脊柱疾病研究北京市重点实验室;
【基金】：国家自然科学基金项目(81672133,81641079) 国家高技术研究发展计划(2015AA020304)
【分类号】：R73-36

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