低浓度阿司匹林通过活化ERK促进肺癌PC14细胞增殖

发布时间：2019-03-04 14:18

【摘要】：目的观察阿司匹林对人肺癌细胞PC14增殖的影响并探讨其作用机制。方法以0、1、2、4、8、16 mmol/L阿司匹林处理肺癌PC14和A549细胞,48 h后以MTT法及集落形成实验检测细胞增殖。为检测低浓度阿司匹林对肺癌PC14细胞增殖的影响与ERK活化间的关系,先以10μmol/L PD98059(ERK抑制剂)预处理PC14细胞30 min,随后加入不同浓度阿司匹林继续培养48 h或7 d,以MTT法及集落形成实验检测细胞增殖情况,并以蛋白质免疫印迹法检测低浓度阿司匹林对ERK活化的影响。结果低浓度的阿司匹林可以促进肺癌PC14细胞和A549细胞的增殖并明显增强PC14细胞ERK的活化;而PD98059预处理组能明显地抑制阿司匹林诱导的PC14细胞的增殖和存活。结论低浓度阿司匹林可促进人肺癌PC14细胞增殖,其机制可能与其增强ERK活化有关。
[Abstract]:Objective To observe the effect of aspirin on the proliferation of human lung cancer cell PC14 and to explore its mechanism of action. Methods Lung cancer PC14 and A549 cells were treated with 0,1,2,4,8,16 mmol/ L aspirin, and the cell proliferation was detected by MTT and colony formation in 48 h. In order to detect the relationship between the effect of low-concentration aspirin on the proliferation of PC14 cells and the activation of ERK, the PC14 cells were pre-treated with 10. m u.mol/ L PD98059 (ERK inhibitor) for 30 min, and then the cells were cultured for 48 h or 7 days with different concentrations of aspirin, and the proliferation of cells was detected by MTT and colony formation. The effect of low-concentration aspirin on the activation of ERK was detected by Western blot. Results The low concentration of aspirin can promote the proliferation of PC14 and A549 cells of lung cancer and enhance the activation of PC14 cells, and the pretreatment group of PD98059 can significantly inhibit the proliferation and survival of PC14 cells induced by aspirin. Conclusion Low-concentration aspirin can promote the proliferation of human lung cancer PC14 cells, and its mechanism may be related to the enhancement of ERK activation.
【作者单位】：锦州医科大学药学院;锦州医科大学护理学院;锦州医科大学医学教育研究所;
【分类号】：R734.2

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