汉防己甲素对胶质瘤细胞的抑制作用

发布时间：2019-03-05 18:01

【摘要】：目的探讨汉防己甲素(Tet)对胶质瘤的作用及其机制。方法 MTT实验与接种肿瘤鸡胚绒毛尿囊膜(CAM)实验检测Tet对U87人胶质瘤细胞增殖的影响;流式分析汉防己甲素对U87人胶质瘤细胞凋亡、周期的影响;苏木素-伊红(HE)染色分析汉防己甲素对血管新生的影响;免疫组织化学实验检测Tet对增殖细胞核抗原(PCNA)与信号传导及转录激活因子(p-STAT3)分布表达影响;Western印迹实验分析Tet对U87人胶质瘤细胞STAT3蛋白及与凋亡相关蛋白表达的影响。结果增殖细胞核抗原能够抑制U87人胶质瘤细胞增殖,呈时间、剂量依赖性;Tet能够诱导U87细胞凋亡呈剂量依赖性;Tet抑制血管新生;Western印迹结果表明Tet降低STAT3磷酸化,STAT3下游基因包括Bcl-2,Cyclin D1,HIF-α和VEGFA在Tet处理之后表达减少;PARP、caspase-3、酶切caspase-9蛋白表达增加,促凋亡蛋白Bax表达上调。结论 Tet抑制胶质瘤细胞增殖,诱导胶质瘤细胞凋亡,抑制血管新生可能与降低STAT3磷酸化有关。
[Abstract]:Objective to investigate the effect and mechanism of Tetrandrine (Tet) on glioma. Methods MTT assay and chorioallantoic membrane (CAM) assay were used to detect the effect of Tet on the proliferation of U87 human glioma cells, and the effects of Tetrandrine on the apoptosis and cell cycle of U87 human glioma cells were analyzed by flow cytometry. The effects of Tetrandrine on angiogenesis were analyzed by hematoxylin-eosin (HE) staining, and the effects of Tet on the distribution of proliferating cell nuclear antigen (PCNA) and signal transduction and activator of transcription (p-STAT3) were detected by immunohistochemistry. Western blot assay was used to analyze the effect of Tet on the expression of STAT3 protein and apoptosis-related protein in U87 human glioma cells. Results proliferating cell nuclear antigen (PCNA) could inhibit the proliferation of U87 human glioma cells in a time-and dose-dependent manner, Tet could induce U87 cell apoptosis in a dose-dependent manner, and Tet inhibited angiogenesis. The results of Western blot showed that Tet decreased the phosphorylation of STAT3, STAT3 downstream genes including Bcl-2,Cyclin D1, HIF-伪 and VEGFA decreased after Tet treatment, and PARP,caspase-3, digestion increased the expression of caspase-9 protein and up-regulated the expression of pro-apoptotic protein Bax. Conclusion Tet can inhibit the proliferation of glioma cells and induce the apoptosis of glioma cells. The inhibition of angiogenesis may be related to the reduction of STAT3 phosphorylation.
【作者单位】：第四军医大学唐都医院神经外科;
【分类号】：R739.41

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