Kiss-1在胃腺癌组织中的表达及对胃腺癌MKN-45细胞增殖、迁移能力的影响

发布时间：2019-03-20 08:10

【摘要】：目的探讨肿瘤抑制因子(Kiss)-1在胃腺癌组织中的表达及对胃腺癌细胞增殖、迁移能力的影响。方法 qRT-PCR检测50例胃腺癌组织和对应的癌旁组织中Kiss-1水平。分别将Kiss-1小干扰RNA(siRNA Kiss-1)和对照小RNA(siRNA control)、Kiss-1过表达载体(p EGFP-N1-Kiss-1)和对照空载体(p EGFP-N1)转染至胃腺癌细胞株(MKN-45)中,培养48 h后,Western印迹检测细胞中Kiss-1、基质金属蛋白酶(MMP)-9、MMP-2、β-连环蛋白(β-catenin)、C-myc蛋白水平,噻唑蓝(MTT)检测细胞增殖能力,细胞划痕实验检测细胞迁移能力。用Wnt/β-catenin信号通路抑制剂FH535作用胃腺癌细胞后,检测细胞增殖和凋亡情况。结果 Kiss-1在胃腺癌组织中的表达水平显著低于癌旁组织(P0.01)。p EGFP-N1-Kiss-1组细胞存活率和迁移率显著低于p EGFP-N1组,siRNA Kiss-1组显著高于siRNA control组(P0.01)。p EGFP-N1-Kiss-1组细胞中MMP-9、MMP-2、β-catenin、C-myc水平显著低于p EGFP-N1组,siRNA Kiss-1组显著高于siRNA control组(P0.01)。抑制剂作用后的胃腺癌细胞增殖迁移趋势与p EGFP-N1-Kiss-1组一致。结论 Kiss-1在胃腺癌组织中低表达,Kiss-1通过Wnt/β-catenin信号通路抑制胃腺癌细胞增殖迁移能力。
[Abstract]:Objective to investigate the expression of tumor suppressor (Kiss)-1 in gastric adenocarcinoma and its effect on proliferation and migration of gastric adenocarcinoma cells. Methods qRT-PCR was used to detect the level of Kiss-1 in 50 gastric adenocarcinoma tissues and corresponding paracancerous tissues. Kiss-1 small interfering RNA (siRNA Kiss-1, control small RNA (siRNA control), Kiss-1 overexpression vector (p EGFP-N1-Kiss-1) and control empty vector (p EGFP-N1) were transfected into gastric adenocarcinoma cell line (MKN-45), respectively. After 48 hours of culture, the levels of Kiss-1, matrix metalloproteinases (MMP)-9, MMP-2, 尾-catenin), C-myc (尾-catenin), C-myc protein) in the cells were detected by Western blot, and the proliferation ability of the cells was detected by thiazolyl blue (MTT). The ability of cell migration was detected by cell scratch test. Wnt/ 尾-catenin signaling pathway inhibitor FH535 was used to detect the proliferation and apoptosis of gastric adenocarcinoma cells. Results the expression level of Kiss-1 in gastric adenocarcinoma tissues was significantly lower than that in paracancerous tissues (P0.01). The cell survival rate and migration rate in). P EGFP-N1-Kiss-1 group were significantly lower than those in p-EGFP-N1 group. The level of MMP-9,MMP-2, 尾-catenin,C-myc in siRNA Kiss-1 group was significantly higher than that in siRNA control group (P0.01), and the level of MMP-9,MMP-2, 尾-catenin,C-myc in siRNA Kiss-1 group was significantly higher than that in siRNA control group (P0.01). The proliferation and migration trend of gastric adenocarcinoma cells treated with inhibitor was consistent with that of p-EGFP-N1-Kiss-1 group. Conclusion the expression of Kiss-1 is low in gastric adenocarcinoma tissues. Kiss-1 inhibits the proliferation and migration of gastric adenocarcinoma cells through Wnt/ 尾-catenin signaling pathway.
【作者单位】：河北医科大学第一医院病理科;河北医科大学第一医院骨科;
【基金】：河北省卫计委青年科技课题(20150642)
【分类号】：R735.2

【相似文献】