射频加热增强前列腺癌局部化疗疗效的实验研究

发布时间：2019-03-31 17:41

【摘要】：目的建立一种用射频加热(radiofrequency heat, RFH)来增强前列腺癌局部化疗疗效的新的介入治疗方法。材料和方法本实验分为体内实验和体外实验两部分。体外实验部分：将体外培养的人前列腺癌细胞株分为4组：(1)对照组；(2)仅射频加热组；(3)仅化疗组(多西紫杉醇)；(4)射频加热-化疗联合治疗组。各组细胞经不同治疗方法处理后,MTS比色法观察对比射频加热-化疗联合治疗组与其他3组肿瘤细胞增殖水平的差异。体内实验部分：首先建立裸鼠人前列腺癌皮下移植瘤模型,将其分为4组(每组n=6):(1)对照组；(2)仅射频加热组;(3)仅化疗组(多西紫杉醇)；(4)射频加热-化疗联合治疗组。在治疗前、治疗后1周及2周用磁共振扫描监测各组移植瘤模型的肿瘤体积,最后从病理组织水平分析证实各组肿瘤组织的凋亡水平。结果体外实验部分：在射频加热-化疗联合治疗组中,人前列腺癌细胞增殖水平较其他3组明显降低(P0.05)；体内实验部分：(1)射频加热-化疗联合治疗后小鼠移植瘤体积与其他3组相比明显缩小(P0.05)；肿瘤组织TUNEL凋亡检测表明联合治疗组治疗后移植瘤凋亡水平较其他组明显升高(P0.05)。结论本实验从体外和体内两方面验证实了射频加热可以增强前列腺癌局部化疗疗效,为下一步建立影像引导下,联合RFH与肿瘤局部化疗两种治疗手段来清除肿瘤消融边界区瘤组织这一新的肿瘤介入治疗技术奠定了基础,新型的介入治疗方法可能为前列腺癌患者提供新的治疗方案。。
[Abstract]:Objective to establish a new interventional therapy for enhancing the efficacy of local chemotherapy for prostate cancer with radiofrequency heating (radiofrequency heat, RFH). Materials and methods this experiment is divided into two parts: in vivo experiment and in vitro experiment. In vitro experiment part: human prostate cancer cell line cultured in vitro was divided into 4 groups: (1) control group; (2) radiofrequency heating group only; (3) only chemotherapy group (docetaxel); (4) combined radiofrequency heating and chemotherapy group. After the cells in each group were treated with different methods, the proliferation level of tumor cells in the radiofrequency heating-chemotherapy combined therapy group was compared with that in the other three groups by MTS colorimetry. In vivo part: the nude mice model of human prostate cancer transplanted subcutaneously was firstly established and divided into 4 groups (n = 6): (1) control group, (2) radiofrequency heating group only, (3) chemotherapy group (docetaxel only), 3) chemotherapy group (docetaxel group), 3) chemotherapy group (docetaxel group). (4) radiofrequency heating combined with chemotherapy group. Before and 1 and 2 weeks after treatment, the tumor volume of the transplanted tumor model was monitored by magnetic resonance imaging (MRI). Finally, the apoptosis level of the tumor tissue was confirmed by pathological analysis. Results in the part of in vitro experiment: the proliferation level of human prostate cancer cells in the radiofrequency heating-chemotherapy group was significantly lower than that in the other three groups (P0.05). In vivo experiment part: (1) after radiofrequency heating-chemotherapy combined treatment, the transplanted tumor volume of mice was significantly smaller than that of the other three groups (P0.05); The apoptotic level of transplanted tumor in the combined treatment group was significantly higher than that in the other groups (P0.05). The TUNEL apoptosis in the tumor tissue was significantly higher than that in the other groups. Conclusion radiofrequency heating can enhance the efficacy of local chemotherapy for prostate cancer in vitro and in vivo. The combination of RFH and local chemotherapy has laid a foundation for the removal of tumor tissue in the tumor ablation boundary region. The new interventional therapy may provide a new therapy for prostate cancer patients.
【学位授予单位】：浙江大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R737.25

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