ZNF580和VEGF在胃癌中的表达及其与临床病理特征及预后的相关性研究
发布时间：2019-04-18 06:47
【摘要】：目的:研究锌指转录因子580(ZNF580)在胃癌中的表达状况,初步探讨其在胃癌发生、发展过程中所扮演的的角色,包括其对于胃癌所造成的影响和血管内皮生长因子(VEGF)之间,潜存的相互联系。方法:将山西医科大学第一医院2010年1月到2013年2月期间,通过手术切除的、病理确诊的144例胃癌以及癌旁组织(距癌肿5cm)的石蜡样本,作为试验研究对象,依据制作芯片的方法并整合免疫组织化学手段,测验ZNF580与VEGF蛋白在胃癌之中的蛋白表达程度。结果:ZNF580和VEGF在144例胃癌中的阳性率(63.9%、57.6%)均明显高于癌旁组(18.8%、13.9%)(P0.05)。在胃癌中ZNF580蛋白和VEGF蛋白的表达分别与肿瘤大小、浸润深度、TNM分期、淋巴结转移有关(P0.05),而它们的表达与年龄、性别及分化程度无关(P0.05)。胃癌组织中ZNF580和VEGF表达正相关(P=0.005)。在单因素生存分析的结果中显示,ZNF580和VEGF蛋白高表达与生存时间均表现为负相关(P0.05)。Cox多元回归分析ZNF580阳性胃癌的预后更差(RR=3.154,P0.05)和VEGF阳性胃癌的预后更差(RR=4.094,P0.05)。结论:ZNF580蛋白在胃癌组织中表现为高表达的状态,且其在胃癌的发生和发展过程中可能与通过与VEGF起协同作用而使胃癌的预后更差,ZNF580结合VEGF也许可以用来做为预测胃癌预后的因子。
[Abstract]:Objective: to study the expression of zinc finger transcription factor 580 (ZNF580) in gastric cancer and its role in the development of gastric cancer, including its influence on gastric cancer and vascular endothelial growth factor (VEGF). Hidden interconnections. Methods: from January 2010 to February 2013, paraffin samples of 144 cases of gastric cancer and paracancerous tissues (5cm), which were surgically resected and pathologically confirmed, were taken as the subjects of the experiment, which were collected from the first Hospital of Shanxi Medical University from January 2010 to February 2013. The expression of ZNF580 and VEGF protein in gastric cancer was measured by the method of microarray and immunohistochemistry. Results: the positive rates of ZNF580 and VEGF in 144 cases of gastric cancer (63.9%, 57.6%) were significantly higher than those in the paracancerous group (18.8%, 13.9%) (P0.05). The expression of ZNF580 protein and VEGF protein were related to tumor size, depth of invasion, TNM stage and lymph node metastasis (P0.05), but their expression was not related to age, sex and differentiation degree (P0.05). There was a positive correlation between the expression of ZNF580 and VEGF in gastric cancer (P < 0. 005). The results of univariate survival analysis showed that there was a negative correlation between the high expression of ZNF580 and VEGF protein and survival time (P0.05). The prognosis of ZNF580 positive gastric cancer was worse than that of ZNF580 positive gastric cancer (RR=3.154,). The prognosis of VEGF-positive gastric cancer was worse than that of P05) (RR=4.094,P0.05). Conclusion: ZNF580 protein is highly expressed in gastric cancer tissues, and it may play a synergistic role with VEGF in the carcinogenesis and development of gastric cancer to make the prognosis of gastric cancer worse. ZNF580 combined with VEGF may be used as a predictor of prognosis in gastric cancer.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R735.2
本文编号：2459838
[Abstract]:Objective: to study the expression of zinc finger transcription factor 580 (ZNF580) in gastric cancer and its role in the development of gastric cancer, including its influence on gastric cancer and vascular endothelial growth factor (VEGF). Hidden interconnections. Methods: from January 2010 to February 2013, paraffin samples of 144 cases of gastric cancer and paracancerous tissues (5cm), which were surgically resected and pathologically confirmed, were taken as the subjects of the experiment, which were collected from the first Hospital of Shanxi Medical University from January 2010 to February 2013. The expression of ZNF580 and VEGF protein in gastric cancer was measured by the method of microarray and immunohistochemistry. Results: the positive rates of ZNF580 and VEGF in 144 cases of gastric cancer (63.9%, 57.6%) were significantly higher than those in the paracancerous group (18.8%, 13.9%) (P0.05). The expression of ZNF580 protein and VEGF protein were related to tumor size, depth of invasion, TNM stage and lymph node metastasis (P0.05), but their expression was not related to age, sex and differentiation degree (P0.05). There was a positive correlation between the expression of ZNF580 and VEGF in gastric cancer (P < 0. 005). The results of univariate survival analysis showed that there was a negative correlation between the high expression of ZNF580 and VEGF protein and survival time (P0.05). The prognosis of ZNF580 positive gastric cancer was worse than that of ZNF580 positive gastric cancer (RR=3.154,). The prognosis of VEGF-positive gastric cancer was worse than that of P05) (RR=4.094,P0.05). Conclusion: ZNF580 protein is highly expressed in gastric cancer tissues, and it may play a synergistic role with VEGF in the carcinogenesis and development of gastric cancer to make the prognosis of gastric cancer worse. ZNF580 combined with VEGF may be used as a predictor of prognosis in gastric cancer.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R735.2
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