长链非编码H19靶向调节miR-107通过Notch通路对肺癌的侵袭迁移的影响
发布时间：2019-04-18 06:41
【摘要】：目的:探讨长链非编码H19靶向调节miR-107通过Notch通路对肺癌的侵袭迁移的影响情况。方法:q PCR检测肺癌和癌旁组织、不同肺癌细胞中H19的表达情况;Transwell侵袭实验检测沉默H19后肺癌细胞侵袭能力的变化;划痕实验检测沉默H19后肺癌细胞迁移能力的变化;双荧光素酶报告基因检测H19与miR-107的相互作用;q PCR检测肺癌和癌旁组织、不同肺癌细胞中miR-107的表达情况;Transwell侵袭实验检测沉默H19后miR-107对肺癌细胞侵袭能力的影响;划痕实验检测沉默H19后miR-107肺癌细胞迁移能力的影响;裸鼠皮下成瘤检测沉默H19后miR-107对肺癌成瘤大小以及体积的影响。Western blot检测沉默H19后Notch通路蛋白的表达情况。结果:与癌旁组织相比,肺癌组织中H19表达明显增高;肺癌细胞A549中H19表达水平最高;沉默H19可以抑制肺癌细胞侵袭和迁移能力;H19能与miR-107的3'UTR特异性结合;与癌旁组织相比,肺癌组织中miR-107表达明显降低;沉默H19后,抑制miR-107可以促进肺癌细胞侵袭和迁移能力;与H19-siRNA组相比,H19-siRNA+miR-107-inhibitor组荷瘤小鼠肿瘤体积和重量都明显增大。沉默H19后Notch通路蛋白表达情况相应下调。结论:H19在肺癌发生发展过程中起重要作用,H19可以靶向调节miR-107通过Notch信号通路调控肺癌细胞的侵袭和迁移能力。
[Abstract]:Aim: to investigate the effect of long-chain non-coding H19 target-regulated miR-107 on invasion and migration of lung cancer through Notch pathway. Methods: q PCR was used to detect the expression of H19 in lung cancer tissues and different lung cancer cells, Transwell invasion test was used to detect the invasion ability of lung cancer cells after silencing H19, scratch test was used to detect the changes of migration ability of lung cancer cells after silencing H19. The interaction between H19 and miR-107 was detected by double luciferase reporter gene.; q PCR was used to detect the expression of miR-107 in lung cancer and paracancerous tissues, and Transwell invasion assay was used to detect the effect of miR-107 on invasion ability of lung cancer cells after silencing H19. The migration ability of miR-107 lung cancer cells after silencing H19 was detected by scratch assay, and the effect of miR-107 on tumor size and volume after H19 silencing was detected in nude mice. Western blot was used to detect the expression of Notch pathway protein after H19 silencing. Results: compared with the paracancerous tissues, the expression of H19 in lung cancer tissues was significantly higher than that in lung cancer tissues, the expression of H19 in A549 cells was the highest, the ability of invasion and migration of lung cancer cells was inhibited by silencing H19, and H19 could specifically bind to 3'UTR of miR-107. Compared with the adjacent tissues, the expression of miR-107 in lung cancer tissues was significantly decreased, and the inhibition of miR-107 could promote the invasion and migration of lung cancer cells after silencing H19. Compared with H19-siRNA group, the tumor volume and weight in H19-siRNA miR-107-inhibitor group increased significantly. The expression of Notch pathway protein was down-regulated after H19 silencing. Conclusion: H19 plays an important role in the carcinogenesis and development of lung cancer. H19 can target-regulate miR-107 through Notch signaling pathway to regulate the invasion and migration of lung cancer cells.
【作者单位】： 河南科技大学第一附属医院胸外科;河南科技大学第一附属医院风湿科;
【分类号】：R734.2
[Abstract]:Aim: to investigate the effect of long-chain non-coding H19 target-regulated miR-107 on invasion and migration of lung cancer through Notch pathway. Methods: q PCR was used to detect the expression of H19 in lung cancer tissues and different lung cancer cells, Transwell invasion test was used to detect the invasion ability of lung cancer cells after silencing H19, scratch test was used to detect the changes of migration ability of lung cancer cells after silencing H19. The interaction between H19 and miR-107 was detected by double luciferase reporter gene.; q PCR was used to detect the expression of miR-107 in lung cancer and paracancerous tissues, and Transwell invasion assay was used to detect the effect of miR-107 on invasion ability of lung cancer cells after silencing H19. The migration ability of miR-107 lung cancer cells after silencing H19 was detected by scratch assay, and the effect of miR-107 on tumor size and volume after H19 silencing was detected in nude mice. Western blot was used to detect the expression of Notch pathway protein after H19 silencing. Results: compared with the paracancerous tissues, the expression of H19 in lung cancer tissues was significantly higher than that in lung cancer tissues, the expression of H19 in A549 cells was the highest, the ability of invasion and migration of lung cancer cells was inhibited by silencing H19, and H19 could specifically bind to 3'UTR of miR-107. Compared with the adjacent tissues, the expression of miR-107 in lung cancer tissues was significantly decreased, and the inhibition of miR-107 could promote the invasion and migration of lung cancer cells after silencing H19. Compared with H19-siRNA group, the tumor volume and weight in H19-siRNA miR-107-inhibitor group increased significantly. The expression of Notch pathway protein was down-regulated after H19 silencing. Conclusion: H19 plays an important role in the carcinogenesis and development of lung cancer. H19 can target-regulate miR-107 through Notch signaling pathway to regulate the invasion and migration of lung cancer cells.
【作者单位】： 河南科技大学第一附属医院胸外科;河南科技大学第一附属医院风湿科;
【分类号】：R734.2
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