小细胞肺癌干细胞和肿瘤组织中HIF-1α和HIF-2α的表达及意义

发布时间：2019-04-28 17:19

【摘要】：目的观察小细胞肺癌干细胞和肿瘤组织中HIF-1α和HIF-2α的表达,并探讨其临床意义。方法采用干细胞无血清培养技术富集小细胞肺癌细胞系H446第3代肿瘤球细胞作为肿瘤干细胞;实时荧光定量PCR法检测H446干细胞中HIF-1α和HIF-2αmRNA的表达;免疫荧光法检测H446干细胞中HIF-1α和HIF-2α蛋白表达;免疫组化SP法检测小细胞肺癌组织中HIF-1α和HIF-2α蛋白表达。结果小细胞肺癌干细胞HIF-2αmRNA表达上调(P0.05),而HIF-1αmRNA表达下调(P0.05);小细胞肺癌干细胞中HIF-2α蛋白呈阳性,而HIF-1α蛋白呈阴性;小细胞肺癌组织中HIF-1α的阳性率为46.7%(28/60),HIF-2α阳性率为25%(15/60)。相关分析结果显示HIF-2α与小细胞肺癌干细胞标志物u PAR呈正相关且两者共同表达于小细胞肺癌坏死组织周围区域;HIF-2α与小细胞肺癌肿瘤直径和远处转移有关(P0.05),而HIF-1α与患者年龄、性别、肿瘤直径、淋巴结转移、胸膜侵袭和远处转移无关(P0.05)。结论 HIF-2α在小细胞肺癌干细胞中表达上调且与小细胞肺癌干细胞标志物u PAR呈正相关,与小细胞肺癌肿瘤直径和远处转移相关,提示HIF-2α表达可能与小细胞肺癌干细胞样特征有关。
[Abstract]:Objective to observe the expression of HIF-1 伪 and HIF-2 伪 in stem cells and tumor tissues of small cell lung cancer and to explore its clinical significance. Methods small cell lung cancer cell line H446 was enriched by serum-free culture of stem cells, and the expression of HIF-1 伪 and HIF- 2 伪 in H446 stem cells was detected by real-time fluorescence quantitative PCR (real-time fluorescence quantitative polymerase chain reaction). The expression of HIF-1 伪 and HIF-2 伪 in H446 stem cells was detected by immunofluorescence method, and the expression of HIF-1 伪 and HIF-2 伪 in small cell lung cancer was detected by immunohistochemistry SP method. Results the expression of HIF-2 伪 mRNA in small cell lung cancer stem cells was up-regulated (P0.05), while the expression of HIF-1 伪 mRNA was down-regulated (P0.05), HIF-2 伪 protein was positive in small cell lung cancer stem cells, while HIF-1 伪 protein was negative in small cell lung cancer stem cells. The positive rate of HIF-1 伪 and HIF-2 伪 in small cell lung cancer was 46.7% (28 / 60) and 25% (15 / 60) respectively. The results of correlation analysis showed that HIF-2 伪 was positively correlated with the stem cell marker u PAR of small cell lung cancer, and both were co-expressed in the surrounding area of necrotic tissue of small cell lung cancer. HIF-2 伪 was correlated with tumor diameter and distant metastasis of small cell lung cancer (P0.05), while HIF-1 伪 was not associated with age, sex, tumor diameter, lymph node metastasis, pleural invasion and distant metastasis (P0.05). Conclusion the up-regulated expression of HIF-2 伪 in small cell lung cancer stem cells is positively correlated with the stem cell marker u-PAR of small cell lung cancer, and is related to the tumor diameter and distant metastasis of small cell lung cancer. These results suggest that the expression of HIF-2 伪 may be related to the stem cell-like characteristics of small cell lung cancer.
【作者单位】：天津医科大学基础医学院教学中心;
【分类号】：R734.2

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