血清miR-143和miR-145在胰腺癌中的表达水平和临床意义

发布时间：2019-04-28 19:46

【摘要】：目的1.通过检测miR-143、miR-145在胰腺癌患者血清中的表达情况,比较miR-143、mi R-145表达水平在胰腺癌患者及健康志愿者血清中的差异。2.分析血清mi R-143、miR-145表达水平与胰腺癌患者临床参数的相关性。方法收集30例胰腺癌患者和30例健康志愿者的血清,采用实时荧光定量PCR方法分别检测胰腺癌患者血清、健康志愿者血清中miR-143、miR-145的表达水平。收集相关临床资料,根据胰腺癌患者临床病例参数(年龄、性别、肿瘤部位、肿瘤大小、TMN分期)对所收集的标本进行分组,采用SPSS19.0进行统计学分析,探究胰腺癌血清中miR-143、mi R-145表达水平与其临床参数的相关性。结果胰腺癌患者血清中mi R-143、miR-145表达水平显著低于健康志愿者,差异有统计学意义(P0.05)。患者血清中miR-143、miR-145存在显著正相关关系(P0.05)。logistics回归分析显示血清miR-143是胰腺癌发病的保护因素(P=0.036,OR=0.269)。胰腺癌患者血清miR-143、miR-145的表达水平在年龄、性别、肿瘤平均直径、肿瘤部位、肿瘤TMN分期上的差异均无统计学意义(P0.05)。结论1.胰腺癌患者血清中mi R-143、mi R-145表达水平显著低于健康志愿者,血清miR-143与mi R-145有显著相关性,且血清miR-143是胰腺癌发病的保护因素。2.血清mi R-143、miR-145表达水平与胰腺癌肿瘤标志物CA199及临床预后指标不存在相关性。
[Abstract]:Purpose 1. By detecting the expression of miR-143,miR-145 in serum of patients with pancreatic cancer, we compared the expression of miR-143,miR-145 in serum of patients with pancreatic cancer and healthy volunteers. To analyze the correlation between the expression of serum miR- 143, miR, 145 and clinical parameters in patients with pancreatic cancer. Methods the serum samples of 30 patients with pancreatic cancer and 30 healthy volunteers were collected. The expression of miR-143,miR-145 in the serum of pancreatic cancer patients and healthy volunteers was detected by real-time fluorescence quantitative PCR (real-time fluorescence quantitative PCR). According to the clinical parameters of pancreatic cancer patients (age, sex, tumor location, tumor size, TMN stage), the collected samples were divided into groups and analyzed statistically with SPSS19.0, and the clinical data were collected according to the clinical case parameters (age, sex, tumor location, tumor size, and tumor stage). Objective: to investigate the correlation between the expression of miR-143,mi-R-145 in serum of pancreatic cancer and its clinical parameters. Results the expression levels of miR- 143 and miRc 145 in serum of pancreatic cancer patients were significantly lower than those of healthy volunteers (P0.05). There was a significant positive correlation between miR-143,miR-145 in serum of patients (P0.05). Logistic regression analysis showed that serum miR-143 was a protective factor of pancreatic cancer (P = 0.036, OR = 0.269). There was no significant difference in serum miR-143,miR-145 expression in patients with pancreatic cancer in age, sex, average tumor diameter, tumor location and tumor TMN stage (P0.05). Conclusion 1. The expression of miR-143 and miR- 145 in serum of pancreatic cancer patients was significantly lower than that of healthy volunteers. Serum miR-143 was significantly correlated with miR- 145, and serum miR-143 was the protective factor of pancreatic cancer. There was no correlation between the expression of serum miR- 143 and miR 伪 145 and the tumor markers CA199 and clinical prognosis of pancreatic cancer.
【学位授予单位】：广西医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R735.9

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