miR-105-1在原发性肝细胞癌中表达的研究
发布时间：2019-05-31 20:11
【摘要】：目的定量分析miRNA-105-1(miR-105-1)在原发性肝细胞癌(HCC)及癌旁正常组织中表达水平的差异。方法取患者手术新鲜组织标本(10对T-N配对组织和另外40例原发性肝癌组织)提取RNA,采用实时荧光定量PCR(RT-q PCR)技术检测标本中miR-105-1基因表达量。并下载公共基因表达数据库(GEO)芯片数据加以验证,通过2-ΔΔCt方法计算得到。结果实验结果表明在10对配对的HCC组织中的miR-105-1的表达量显著低于癌旁正常组织,差异有统计学意义(P=0.046)。在50个HCC组织的表达量显著低于10个癌旁组织,差异有统计学意义(P=0.031)。GEO数据库芯片GSE22058-GPL10457中包含96个配对的HCC与癌旁正常组织,miR-105-1在HCC中下调,差异有统计学意义(P=0.035)。GSE21362中包含73个配对的HCC与癌旁正常组织,miR-105-1在HCC中也是下调的,差异有统计学意义(P=0.042)。结论 miR-105-1在原发性肝癌患者中较正常人群表达量低,可能是HCC患者发生发展的重要分子之一,并可以作为判断预后的一个指标和治疗的一个新靶点。
[Abstract]:Objective to quantitatively analyze the expression of miRNA-105-1 (miR-105-1) in primary hepatocellular carcinoma (HCC) and adjacent normal tissues. Methods RNA, was extracted from fresh tissues of patients (10 pairs of T / N matched tissues and 40 cases of primary liver cancer). The expression of miR-105-1 gene was detected by real-time fluorescence quantitative PCR (RT-q PCR). The (GEO) chip data of the common gene expression database were downloaded and verified and calculated by 2-螖 Ct method. Results the results showed that the expression of miR-105-1 in 10 pairs of HCC tissues was significantly lower than that in normal tissues adjacent to cancer (P 鈮，
本文编号：2489989
[Abstract]:Objective to quantitatively analyze the expression of miRNA-105-1 (miR-105-1) in primary hepatocellular carcinoma (HCC) and adjacent normal tissues. Methods RNA, was extracted from fresh tissues of patients (10 pairs of T / N matched tissues and 40 cases of primary liver cancer). The expression of miR-105-1 gene was detected by real-time fluorescence quantitative PCR (RT-q PCR). The (GEO) chip data of the common gene expression database were downloaded and verified and calculated by 2-螖 Ct method. Results the results showed that the expression of miR-105-1 in 10 pairs of HCC tissues was significantly lower than that in normal tissues adjacent to cancer (P 鈮，
本文编号：2489989
本文链接：https://www.wllwen.com/yixuelunwen/zlx/2489989.html
