卡波西肉瘤中血管内皮生长因子A的表达及意义
发布时间：2019-06-09 15:48
【摘要】：目的:明确卡波西肉瘤组织中血管内皮生长因子A(VEGFA)的表达情况,探讨VEGFA的表达与卡波西肉瘤临床病理特征之间的关系。方法:选取新疆地区30例卡波西肉瘤石蜡组织、21例血管瘤石蜡组织及15例正常皮肤石蜡组织,分别采用实时荧光定量PCR及免疫组化两种方法检测三种石蜡组织中VEGFA m RNA及蛋白的表达水平。结果:VEGFA m RNA在卡波西肉瘤组织中的表达水平为11.67±1.80,在血管瘤组织中的表达水平为10.90±1.22,两组比较差异无统计学意义(P0.05);VEGFA m RNA在正常皮肤组织的表达水平为1.02±0.20,卡波西肉瘤组织中VEGFA m RNA的表达高于正常皮肤组织,差异具有统计学意义(P0.05);VEGFA蛋白在卡波西肉瘤组织、血管瘤组织、正常皮肤组织中的阳性表达率分别为83.3%、71.4%、6.7%,VEGFA蛋白在卡波西肉瘤组织中的表达与正常皮肤组织比较,差异具有统计学意义(P0.05),但与血管瘤组织比较无明显差异(P0.05);VEGFA的表达与卡波西肉瘤患者年龄、性别、HHV-8感染、HIV感染、皮损面积差异无统计学意义(P0.05),而与病理分期呈正相关(rs=0.918,P=0.000),即结节期高于斑片期、斑块期,斑块期高于斑片期,差异有统计学意义(P0.05)。结论:VEGFA在卡波西肉瘤组织中的表达明显升高,且与病理分期呈正相关,提示VEGFA可能与卡波西肉瘤血管的发生、发展有密切关系,这可以为卡波西肉瘤的发病机制提供一个新的研究方向。
[Abstract]:Objective: to investigate the expression of vascular endothelial growth factor A (VEGFA) in Kaposi's sarcomas and to explore the relationship between the expression of VEGFA and the clinicopathological features of Kaposi's sarcomas. Methods: paraffin tissue of 30 cases of Kaposi's sarcomas, 21 cases of hemangioma and 15 cases of normal skin paraffin tissue were selected in Xinjiang. The expression of VEGFA m RNA and protein in three kinds of paraffin tissues were detected by real-time fluorescence quantitative PCR and immunohistochemistry, respectively. Results the expression level of: VEGFA m RNA in Kaposi's sarcomas was 11.67 卤1.80, and that in hemangioma was 10.90 卤1.22. There was no significant difference between the two groups (P 0.05). The expression level of VEGFA m RNA in normal skin tissue was 1.02 卤0.20. the expression of VEGFA m RNA in Kaposi's sarcomas was higher than that in normal skin tissue, the difference was statistically significant (P 0.05). The positive expression rates of VEGFA protein in Kaposi's sarcomas, angiomas and normal skin tissues were 83. 3%, 71. 4% and 6. 7%, respectively. the expression of VEGFA protein in Kaposi's sarcomas was significantly higher than that in normal skin tissues. The difference was statistically significant (P 0.05), but there was no significant difference between the two groups (P 0.05). The expression of VEGFA was not significantly different from age, sex, HHV-8 infection, HIV infection and skin lesion area in patients with Kaposi's sarcomas (P 0.05), but positively correlated with pathological stage (rs=0.918,P=0.000), that is, nodular stage was higher than plaque stage. Plaque stage, plaque stage was higher than plaque stage, the difference was statistically significant (P 0.05). Conclusion: the expression of VEGFA in Kaposi's sarcomas is significantly increased, which is positively correlated with pathological stage, suggesting that VEGFA may be closely related to the occurrence and development of Kaposi's sarcomas. This can provide a new research direction for the pathogenesis of Kaposi's sarcomas.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R730.26
[Abstract]:Objective: to investigate the expression of vascular endothelial growth factor A (VEGFA) in Kaposi's sarcomas and to explore the relationship between the expression of VEGFA and the clinicopathological features of Kaposi's sarcomas. Methods: paraffin tissue of 30 cases of Kaposi's sarcomas, 21 cases of hemangioma and 15 cases of normal skin paraffin tissue were selected in Xinjiang. The expression of VEGFA m RNA and protein in three kinds of paraffin tissues were detected by real-time fluorescence quantitative PCR and immunohistochemistry, respectively. Results the expression level of: VEGFA m RNA in Kaposi's sarcomas was 11.67 卤1.80, and that in hemangioma was 10.90 卤1.22. There was no significant difference between the two groups (P 0.05). The expression level of VEGFA m RNA in normal skin tissue was 1.02 卤0.20. the expression of VEGFA m RNA in Kaposi's sarcomas was higher than that in normal skin tissue, the difference was statistically significant (P 0.05). The positive expression rates of VEGFA protein in Kaposi's sarcomas, angiomas and normal skin tissues were 83. 3%, 71. 4% and 6. 7%, respectively. the expression of VEGFA protein in Kaposi's sarcomas was significantly higher than that in normal skin tissues. The difference was statistically significant (P 0.05), but there was no significant difference between the two groups (P 0.05). The expression of VEGFA was not significantly different from age, sex, HHV-8 infection, HIV infection and skin lesion area in patients with Kaposi's sarcomas (P 0.05), but positively correlated with pathological stage (rs=0.918,P=0.000), that is, nodular stage was higher than plaque stage. Plaque stage, plaque stage was higher than plaque stage, the difference was statistically significant (P 0.05). Conclusion: the expression of VEGFA in Kaposi's sarcomas is significantly increased, which is positively correlated with pathological stage, suggesting that VEGFA may be closely related to the occurrence and development of Kaposi's sarcomas. This can provide a new research direction for the pathogenesis of Kaposi's sarcomas.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R730.26
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