TP53INP1高表达在砷剂杀伤肺腺癌细胞中的增敏作用
发布时间：2019-06-18 12:31
【摘要】：目的构建TP53INP1高表达的肺腺癌A549细胞,初步研究提高TP53INP1的表达能否提高砷剂对肺腺癌细胞的杀伤作用。方法构建TP53INP1重组真核表达质粒,以慢病毒为载体将其转染到A549细胞,建立稳定高表达TP53INP1基因的A549-TP53INP1细胞株,以流式细胞术和MTT实验检测As_2O_3处理后A549-TP53INP1细胞的凋亡和存活率。结果成功构建稳定A549-TP53INP1细胞株。流式细胞术结果显示,相同剂量As2O3作用下(5~40μmol/L),A549-TP53INP1细胞凋亡率[(15.6±3.5)%]显著高于A549细胞[(10.0±2.5)%](P0.05)。MTT实验显示,As_2O_3处理后A549-TP53INP1细胞的IC50值[(44.64±6.84)μmol/L]显著低于A549细胞[(54.25±6.13)μmol/L](P0.05)。结论 TP53INP1高表达可增加砷剂对肺腺癌细胞的杀灭作用,提示TP53INP1可作为砷剂治疗肺癌的增敏靶点。
[Abstract]:Objective to construct lung cancer cell line A549 with high expression of TP53INP1 and to study whether increasing the expression of TP53INP1 can increase the killing effect of arsenic on lung adenocarcinoma cell line. Methods the recombinant eukaryotic expression plasmid TP53INP1 was constructed and transformed into A549 cells with lentivirus as vector. The A549-TP53INP1 cell line with stable and high expression of TP53INP1 gene was established. The apoptosis and survival rate of A549-TP53INP1 cells treated with As_2O_3 were detected by flow cytometry and MTT assay. Results stable A549-TP53INP1 cell line was successfully constructed. The results of flow cytometry showed that the apoptosis rate of (5 ~ 40 渭 mol / L), A549-TP53INP1) cells [(15.6 卤3.5)%] was significantly higher than that of A549 cells [(10.0 卤2.5)%] (P 0.05). The IC50 value of A549-TP53INP1 cells treated with As_2O_3 [(44.64 卤6.84) 渭 mol/L] was significantly lower than that of A549 cells [(54.25 卤6.13) 渭 mol/L] (P 0.05). Conclusion the high expression of TP53INP1 can increase the killing effect of arsenic on lung cancer cells, suggesting that TP53INP1 can be used as a sensitizing target for arsenic in the treatment of lung cancer.
【作者单位】： 四川大学华西公共卫生学院环境卫生与职业医学系;
【基金】：国家自然科学基金面上项目(No.81372945)
【分类号】：R734.2
[Abstract]:Objective to construct lung cancer cell line A549 with high expression of TP53INP1 and to study whether increasing the expression of TP53INP1 can increase the killing effect of arsenic on lung adenocarcinoma cell line. Methods the recombinant eukaryotic expression plasmid TP53INP1 was constructed and transformed into A549 cells with lentivirus as vector. The A549-TP53INP1 cell line with stable and high expression of TP53INP1 gene was established. The apoptosis and survival rate of A549-TP53INP1 cells treated with As_2O_3 were detected by flow cytometry and MTT assay. Results stable A549-TP53INP1 cell line was successfully constructed. The results of flow cytometry showed that the apoptosis rate of (5 ~ 40 渭 mol / L), A549-TP53INP1) cells [(15.6 卤3.5)%] was significantly higher than that of A549 cells [(10.0 卤2.5)%] (P 0.05). The IC50 value of A549-TP53INP1 cells treated with As_2O_3 [(44.64 卤6.84) 渭 mol/L] was significantly lower than that of A549 cells [(54.25 卤6.13) 渭 mol/L] (P 0.05). Conclusion the high expression of TP53INP1 can increase the killing effect of arsenic on lung cancer cells, suggesting that TP53INP1 can be used as a sensitizing target for arsenic in the treatment of lung cancer.
【作者单位】： 四川大学华西公共卫生学院环境卫生与职业医学系;
【基金】：国家自然科学基金面上项目(No.81372945)
【分类号】：R734.2
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