不同剂量的维生素C对奥沙利铂抗肿瘤疗效的影响
发布时间：2019-06-25 09:56
【摘要】：目的:探讨不同剂量的维生素C(抗坏血酸,Vitamin C,VC)对奥沙利铂(L-OHP)在人胃癌裸鼠模型中抗肿瘤疗效的影响。方法:建立人胃癌细胞(SGC-7901)裸鼠皮下移植瘤模型,按照随机数字表法分为8组,每组10只。A组腹腔注射5%葡萄糖注射液;B组腹腔注射L-OHP 6 mg/kg;C、D、E组分别腹腔注射低、中、高剂量VC 100、200、400 mg/kg;F、G、H组分别腹腔注射L-OHP 6 mg/kg及低、中、高剂量VC 100、200、400 mg/kg,各组葡萄糖、VC每天给药1次,L-OHP每3天给药1次,连续用药15天。观察裸鼠生活状态,测裸鼠体质量、瘤体积,计算抑瘤率,绘制移植瘤生长曲线;停药后杀死动物,检测血常规、肝、肾功能,行肿瘤、肝病理组织学检查。结果:VC与L-OHP之间存在交互作用(P0.01),呈拮抗性,VC的浓度越高,对L-OHP抑制肿瘤生长的拮抗作用越强。C、D、E组未表现出抑制肿瘤生长的作用(P0.05)。B、F、G、H组表现出抑制肿瘤生长的作用(P0.01),且随VC浓度增高,抑瘤率下降。结论:不同剂量VC均未表现出抑制胃癌细胞生长的作用;VC对L-OHP的化疗疗效有拮抗作用,且呈剂量依赖性。
[Abstract]:Aim: to investigate the effect of different doses of vitamin C (ascorbic acid, Vitamin C, VC) on the antitumor effect of oxaliplatin (L-OHP) in nude mice with human gastric cancer. Methods: the subcutaneous transplantation tumor model of human gastric cancer cell (SGC-7901) in nude mice was established and divided into 8 groups with 10 mice in each group according to the random digital table method. 5% glucose injection was injected intraperitoneally in group A and low, middle and high dose VC 100200400 mg/kg; were injected intraperitoneally in group B and group B with L-OHP 6 mg/kg;C,D,E, respectively. F, G, H group were intraperitoneally injected with L-OHP 6 mg/kg and low, medium and high dose VC 100200400 mg/kg, groups, VC was given once a day, L-OHP was given once every 3 days for 15 days. The living state of nude mice was observed, the body mass and tumor volume of nude mice were measured, the tumor inhibition rate was calculated, and the growth curve of transplanted tumor was drawn. The animals were killed after drug withdrawal, the blood routine, liver and kidney function, tumor and liver pathology were examined. Results: there was interaction between VC and L-OHP (P 0.01). The higher the concentration of VC, the stronger the antagonistic effect on L-OHP inhibition of tumor growth. C, D, E group showed no inhibitory effect on tumor growth (P 0.05). B, F, G, H group showed inhibitory effect on tumor growth (P 0.01), and the tumor inhibition rate decreased with the increase of VC concentration. Conclusion: different doses of VC have no inhibitory effect on the growth of gastric cancer cells, and VC has antagonistic effect on the chemotherapy effect of L-OHP in a dose-dependent manner.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R735.2
本文编号：2505595
[Abstract]:Aim: to investigate the effect of different doses of vitamin C (ascorbic acid, Vitamin C, VC) on the antitumor effect of oxaliplatin (L-OHP) in nude mice with human gastric cancer. Methods: the subcutaneous transplantation tumor model of human gastric cancer cell (SGC-7901) in nude mice was established and divided into 8 groups with 10 mice in each group according to the random digital table method. 5% glucose injection was injected intraperitoneally in group A and low, middle and high dose VC 100200400 mg/kg; were injected intraperitoneally in group B and group B with L-OHP 6 mg/kg;C,D,E, respectively. F, G, H group were intraperitoneally injected with L-OHP 6 mg/kg and low, medium and high dose VC 100200400 mg/kg, groups, VC was given once a day, L-OHP was given once every 3 days for 15 days. The living state of nude mice was observed, the body mass and tumor volume of nude mice were measured, the tumor inhibition rate was calculated, and the growth curve of transplanted tumor was drawn. The animals were killed after drug withdrawal, the blood routine, liver and kidney function, tumor and liver pathology were examined. Results: there was interaction between VC and L-OHP (P 0.01). The higher the concentration of VC, the stronger the antagonistic effect on L-OHP inhibition of tumor growth. C, D, E group showed no inhibitory effect on tumor growth (P 0.05). B, F, G, H group showed inhibitory effect on tumor growth (P 0.01), and the tumor inhibition rate decreased with the increase of VC concentration. Conclusion: different doses of VC have no inhibitory effect on the growth of gastric cancer cells, and VC has antagonistic effect on the chemotherapy effect of L-OHP in a dose-dependent manner.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R735.2
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