血根碱对宫颈癌细胞增殖、凋亡、侵袭、迁移能力影响机制研究

发布时间：2019-06-29 18:14

【摘要】：目的探讨血根碱对宫颈癌细胞增殖、凋亡、侵袭、迁移能力的影响机制。方法分别用MTT法、流式细胞仪、细胞划痕实验、Transwell小室检测血根碱作用后的宫颈癌细胞增殖、凋亡、迁移、侵袭能力。Western blot检测经血根碱作用后宫颈癌细胞中E-Cadherin、PTEN、β-catenin、MMP2的蛋白表达水平。结果 0.6、0.8μmol/L血根碱对宫颈癌细胞增殖具有抑制作用。0.8μmol/L血根碱作用48h后宫颈癌细胞HeLa和Siha凋亡率高达(45.68±2.26)%和(31.89±3.80)%。0.8μmol/L血根碱作用3h后宫颈癌细胞HeLa和Siha黏附率仅有(67.45±2.13)%和(73.59±2.61)%。0.8μmol/L血根碱作用16h后宫颈癌细胞HeLa和Siha侵袭数目仅有(39.64±1.98)个和(43.87±2.83)个。经血根碱作用后的宫颈癌细胞中E-Cadherin和PTEN的表达量增加,β-catenin和MMP2的表达量减弱。结论血根碱对宫颈癌细胞具有抑制增殖及促凋亡作用,其机制可能与黏附蛋白E-Cadherin、β-catenin和PTEN、MMP2有关。
[Abstract]:Objective to investigate the mechanism of effect of hemophylline on proliferation, apoptosis, invasion and migration of cervical cancer cells. Methods MTT assay, flow cytometry, cell scratch test and Transwell chamber were used to detect the proliferation, apoptosis, migration and invasiveness of cervical cancer cells treated with hemophylline. Western blot was used to detect the protein expression of E 鈮，

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