长链非编码核糖核酸Malat-1、p21和GAS5在大肠癌组织中的表达及其诊断价值

发布时间：2019-07-02 11:48

【摘要】：目的研究长链非编码核糖核酸肺癌转移相关转录本1(metastasis-associated lung adenocarcinoma transcript1,Malat-1)、p21和生长停滞特异性转录因子5(growth arrest-specific 5,GAS5)在大肠癌中的表达及诊断价值。方法选择大肠癌早期和中晚期病变(含癌旁病变)、癌前病变、良性腺瘤和非肿瘤性病变各33例内镜下或手术切除标本,行RT-PCR法检测组织中Malat-1、p21和GAS5的表达水平,ELISA法检测血清癌胚抗原(carcino-embryonic antigen,CEA)和糖链抗原(carbohydrate antigen19-9,CA19-9)的表达水平。结果 RT-PCR法显示大肠癌早期、中晚期和癌前病变组中Malat-1、p21和GAS5的表达水平显著高于癌旁病变、良性腺瘤和非肿瘤性病变,中晚期组最高(P0.05);非肿瘤性病变组的CEA和CA19-9水平显著低于其他组(均P0.05),其他5组间比较差异无统计学意义。RT-PCR法检测的Malat-1、p21和GAS5诊断大肠癌的受试者工作曲线(operator characteristic curve,ROC),分析比较曲线下面积(area under the curve,AUC),差异无统计学意义(P0.05)。病理检测结果显示:大肠癌早期、中晚期和癌前病变组各种病理改变显著多于癌旁病变、良性腺瘤和非肿瘤性病变,中晚期组最明显。结论非编码核糖核酸Malat-1、p21和GAS5在大肠癌不同时期组织中有差异性表达,可作为诊断大肠癌和癌前病变的重要指标。
[Abstract]:Objective to study the expression and diagnostic value of long chain non-coding ribonucleic acid lung cancer metastasis related transcript 1 (metastasis-associated lung adenocarcinoma transcript1,Malat-1), p21 and growth stasis specific transcription factor 5 (growth arrest-specific 5, GAS5) in colorectal cancer. Methods 33 cases of early and advanced colorectal cancer lesions (including paracancerous lesions), 33 cases of precancerous lesions, 33 cases of benign adenoma and 33 cases of non-neoplastic lesions were selected. The expression of Malat-1,p21 and GAS5 in tissues was detected by RT-PCR, and the expression of serum carcinoembryonic antigen (carcino-embryonic antigen,CEA) and sugar chain antigen (carbohydrate antigen19-9,CA19-9) were detected by ELISA. Results the expression levels of Malat-1,p21 and GAS5 in early, advanced and precancerous lesions of colorectal cancer were significantly higher than those in paracancerous lesions, benign adenomas and non-neoplastic lesions, and the highest in the middle and late stages of colorectal cancer (P 0.05). The levels of CEA and CA19-9 in the non-neoplastic lesion group were significantly lower than those in the other groups (P 0.05), but there was no significant difference among the other five groups. The working curve (operator characteristic curve,ROC of Malat-1,p21 and GAS5 in the diagnosis of colorectal cancer by RT-PCR), and the area (area under the curve,AUC under the analysis and comparison curve was not significantly different (P 0.05). The results of pathological examination showed that the pathological changes in early, advanced and precancerous lesions of colorectal cancer were significantly higher than those in paracancerous lesions, benign adenoma and non-neoplastic lesions, and the most obvious in the middle and late stages of colorectal cancer. Conclusion non-coding ribonucleic acid Malat-1,p21 and GAS5 are differentially expressed in different stages of colorectal cancer, which can be used as an important index for the diagnosis of colorectal cancer and precancerous lesions.
【作者单位】：湖南省人民医院马王堆院区消化内科;
【分类号】：R735.34

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