胃癌细胞促进网膜脂肪干细胞分化的机制研究
发布时间：2019-07-16 07:39
【摘要】：目的:本实验主要研究在胃癌条件培养基(conditioned medium,CM)诱导下网膜脂肪干细胞(omental-adipose stromal cells,O-ASCs)是否能分化为癌相关成纤维细胞(carcinoma-associated fibroblasts,CAFs),及ERK信号通路在其中的作用。方法:通过诱导分化成骨、成脂及流式细胞鉴定O-ASCs,将O-ASCs与MGC803和SGC7901 CM共培养,通过RT-PCR和Western-blot检测O-ASCs细胞CAFs标志物α-SMA、FSP-1、vimentin,旁分泌因子VEGFA、TGFβ-1、FAP、SDF-1的表达水平。将O-ASCs分为对照组,SGC7901-CM实验组,SGC7901-CM+U0126处理组,12 h后收集细胞。Western blot检测O-ASCs细胞CAFs标志物α-SMA、FSP-1及ERK1/2、p-ERK1/2的表达水平。结果:经鉴定原代培养出的细胞为O-ASCs,在SGC7901 CM和MGC803 CM作用下,CAFs标志物α-SMA、FSP-1、vimentin及旁分泌因子SDF-1、VEGFA、TGFβ-1、FAP表达均有明显增加(P0.05)。与对照组比较SGC7901-CM组α-SMA、FSP-1、p-ERK1/2表达明显增加(P0.05),ERK表达未见明显变化(P0.05)。SGC7901-CM+U0126组与SGC7901-CM组比较,α-SMA、FSP-1及p-ERK1/2的蛋白表达水平明显降低(P0.05),ERK表达变化无统计学意义(P0.05)。结论:O-ASCs通过分化为CAFs及旁分泌作用参与胃癌腹膜转移,ERK信号通路在该过程中发挥了重要作用。
[Abstract]:Aim: to study whether omental adipose stem cells (omental-adipose stromal cells,O-ASCs) can differentiate into cancer-associated fibroblasts (carcinoma-associated fibroblasts,CAFs) induced by gastric cancer conditioned medium (conditioned medium,CM) and the role of ERK signaling pathway. Methods: O-ASCs was co-cultured with MGC803 and SGC7901 CM by inducing osteogenesis, adipogenic and flow cytometry. The expression of CAFs marker 伪-SMA,FSP-1,vimentin, paracrine factor VEGFA,TGF 尾-1, FAPF and SDF 鈮，
本文编号：2514931
[Abstract]:Aim: to study whether omental adipose stem cells (omental-adipose stromal cells,O-ASCs) can differentiate into cancer-associated fibroblasts (carcinoma-associated fibroblasts,CAFs) induced by gastric cancer conditioned medium (conditioned medium,CM) and the role of ERK signaling pathway. Methods: O-ASCs was co-cultured with MGC803 and SGC7901 CM by inducing osteogenesis, adipogenic and flow cytometry. The expression of CAFs marker 伪-SMA,FSP-1,vimentin, paracrine factor VEGFA,TGF 尾-1, FAPF and SDF 鈮，
本文编号：2514931
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