A549与b.End.3细胞共培养对血脑屏障相关的Epsin1、CD309和CD144分子表达的影响
发布时间：2019-07-26 10:55
【摘要】：目的:研究非小细胞肺癌(non-small cell lung cancer,NSCLC)细胞A549与小鼠脑血管内皮细胞b.End.3共培养对b.End.3中Epsin1以及其他相关因子表达量的影响。方法:使用b.End.3小鼠脑血管内皮细胞与非小细胞肺癌A549细胞建立体外血脑屏障模型(blood brain barrier,BBB)、血瘤屏障(blood tumor barrier,BTB)模型。实验分为两组:BBB组、BTB组。使用流式细胞仪分选BTB组中的b.End.3细胞。使用q PCR和Western Blot的方法分别检测分选出的b.End.3细胞中Epsin1、CD309、CD144、Occludin转录和表达水平的变化。结果:与A549细胞共培养后,BTB组跨膜细胞电阻值(transendothelial electrical resistance,TEER)较BBB组下降(P0.05)。共培养后分选出的b.End.3细胞中的Epsin1和CD309、CD144的表达量增高(P0.05),Occludin的表达量下降(P0.05)。结论:A549共培养后体外血脑屏障的开放可能与Epsin1及相关因子的表达增高有关。
[Abstract]:Aim: to study the effect of co-culture of non-small cell lung cancer (non-small cell lung cancer,NSCLC) cell line A549 and mouse cerebrovascular endothelial cell b.End.3 on the expression of Epsin1 and other related factors in b.End.3. Methods: the blood-brain barrier model (blood brain barrier,BBB) and blood-tumor barrier (blood tumor barrier,BTB) model were established by using b.End.3 mouse cerebral vascular endothelial cells and non-small cell lung cancer cell line A549 cells in vitro. The experiment was divided into two groups: BBB group and BTB group. B.End.3 cells in BTB group were separated by flow cytometry. Q PCR and Western Blot methods were used to detect the changes of Epsin1,CD309,CD144,Occludin transcription and expression in the selected b.End.3 cells. Results: after co-culture with A549 cells, the resistance value of transmembrane cells in BTB group was lower than that in BBB group (P 0.05). After co-culture, the expression of Epsin1 and CD309,CD144 in b.End.3 cells increased (P 0.05), and the expression of), Occludin decreased (P 0.05). Conclusion: the opening of blood-brain barrier in vitro after co-culture of A549 may be related to the increased expression of Epsin1 and related factors.
【作者单位】： 昆明医科大学第一附属医院神经外科;昆明医科大学生物化学与分子生物学系;
【基金】：云南省科技厅-昆明医科大学应用基础研究联合专项资金(2015FB033)
【分类号】：R734.2
，
本文编号：2519521
[Abstract]:Aim: to study the effect of co-culture of non-small cell lung cancer (non-small cell lung cancer,NSCLC) cell line A549 and mouse cerebrovascular endothelial cell b.End.3 on the expression of Epsin1 and other related factors in b.End.3. Methods: the blood-brain barrier model (blood brain barrier,BBB) and blood-tumor barrier (blood tumor barrier,BTB) model were established by using b.End.3 mouse cerebral vascular endothelial cells and non-small cell lung cancer cell line A549 cells in vitro. The experiment was divided into two groups: BBB group and BTB group. B.End.3 cells in BTB group were separated by flow cytometry. Q PCR and Western Blot methods were used to detect the changes of Epsin1,CD309,CD144,Occludin transcription and expression in the selected b.End.3 cells. Results: after co-culture with A549 cells, the resistance value of transmembrane cells in BTB group was lower than that in BBB group (P 0.05). After co-culture, the expression of Epsin1 and CD309,CD144 in b.End.3 cells increased (P 0.05), and the expression of), Occludin decreased (P 0.05). Conclusion: the opening of blood-brain barrier in vitro after co-culture of A549 may be related to the increased expression of Epsin1 and related factors.
【作者单位】： 昆明医科大学第一附属医院神经外科;昆明医科大学生物化学与分子生物学系;
【基金】：云南省科技厅-昆明医科大学应用基础研究联合专项资金(2015FB033)
【分类号】：R734.2
，
本文编号：2519521
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