Overexpression of CircRNA BCRC4 Regulates Cell Apoptosis and

发布时间：2021-04-15 15:23

　　Emerging evidence has indicated that circular RNAs（circRNAs） play pivotal roles in the regulation of cellular processes and are found to be aberrantly expressed in a variety of tumors. However, the clinical role of circ RNAs in bladder cancer（BC） and the molecular mechanisms have yet to be fully understood. In this study, the clinical specimens were obtained and the expression level of a circ RNA BCRC4 was detected by real-time PCR in both BC tissues and cell line. The circular RNA over-expressi... 

【文章来源】：Journal of Huazhong University of Science and Technology（Medical Sciences）. 2017,37(06)SCI

【文章页数】：5 页

【文章目录】：
1 MATERIALS AND METHODS
    1.1 Cell Culture and Treatment
    1.2 Tissue Specimens of Patients
    1.3 RNA Extraction and Real-time PCR Assays
    1.4 Cell Viability by CCK-8 Assay
    1.5 Cell Apoptosis Assay
    1.6 Western Blotting Analysis
    1.7 Circ RNA Plasmids Construction
    1.8 Statistical Analysis
2 RESULRS
    2.1 Relative Low-expression of BCRC4 in BC Tissues and Cell Lines
    2.2 Inhibitory Effects of BCRC4 Over-expression on Viability and Apoptosis of BC Cells In Vitro
    2.3 Effects of Overexpression of BCRC4 on Expres-sion of mi R-101 and EZH2
    2.4 Up-regulation of BCRC4 Expression and Dow-n-regulation of EZH2 Expression by GA Treatment
3 DISCUSSION
Conflict of Interest Statement



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