长链非编码RNA H19在食管鳞癌中的表达水平及其临床预后相关性的研究
发布时间：2021-07-06 07:12
食管癌是最致命的恶性肿瘤之一。它是导致肿瘤死亡的第六大原因,也是世界上第八大最常见的肿瘤。主要是因为它极难早期诊断,侵袭能力强,因此预后差以及存活率低。其五年存活率约为15%-25%。更糟糕的是,随着世界范围内人口老龄化的增长,食管癌的发病率也正在逐年上升。中国是食管癌发病率最高的地区之一,尤其以中国河南省安阳市最高。长链非编码RNA（long non-coding RNA,lncRNA）为长度大于200个核苷酸,不具有编码蛋白功能的RNA分子。报道表明,它可以通过与DNA、染色质、蛋白质、RNA等大分子相互作用促进或抑制肿瘤的发生、侵袭和转移,其复杂的作用机制是目前研究的重点和难点。据报道,LncRNAH19在成人组织中表达量低或不表达,但在多种肿瘤中高表达,如大肠癌、乳腺癌、膀胱癌等,且与肿瘤的发生发展密切相关。因此LncH19有可能具有肿瘤早期诊断和预后判断的标志物的临床价值。本课题大规模地使用ISH方法检测食管鳞癌中H19的表达水平,旨在探讨其在食管癌中的临床病理作用。其次运用生物信息学分析方法预测LncRNA H19的靶向miRNA,探讨H19在食管鳞癌中可能的作用机制。方法...
【文章来源】：郑州大学河南省 211工程院校
【文章页数】：65 页
【学位级别】：硕士
【部分图文】：
图１?Ｈ１９原位杂交??
?实验结果???相关，见图２。??＾?１?Ｃ３?水１５一年内死?Ｃ?？〇－，?１?＊＊：４??ｆＭ－?…?■■舰??＇：ＵＵ?＂？?Ｉｉ〇Ｉｊ?ｊ?ｊＩ??图２?ＥＳＣＣ样本中ＬｎｃＨｌ９?ＩＳＨ表达水平柱状图??注：Ａ：两个ＥＳＣＣ组中ＬｎｃＨｌ９ＩＳＨ表达水平差异＾?Ｂ：ＬｎｃＨｌ９表达水平与Ｕ１ＣＣ分期??的关系。＂＊?ｐ?＝?０．〇０ｌ。??表３?ＬｎｃＲＮＡ?ＨＩ９表达与食管鱗癌临床病理特点之间的关系??临床特征?总?弱阳性?中等阳性?强阳性?Ｐ值??（Ｎ＝ｌ２ｌ）?（Ｎ＝ｌ９）?ｆＮ＝６４）?（Ｎ＝３８）??生存分组?０．００丨ａ??术后一年内死亡（％）?６３（５２．１）?６（５．０）?１２（９．９）?４５（３７．２）??术后超过五年生存（％）?５８（４７．９）?２０（１６．５）?２０（１６．５）?１８（１４．９）??年龄?０．１３２ｂ??＞６０（％）?６０（４９．６）?１３（１０．７）?１５（１２．４）?３２（２６．４）??＜６０（％）?６１（５０．４）?１３（１０．７）?１７（１４．０）?３１（２５．６）??性别?０．５９１ｃ??女（％）?６３（５２．１）?１５（１２．４）?１８（１４．９）?３０（２４．８）??男（％）?５８（４７．９）?１?１（９．１）?１４（１１．６）?３３（２７．３）??肿瘤大小?０．００２ａ??＞３ｃｍ（％）?１０７（８８．４）?１８（１４．９）?３０（２４．８）?５９（４８．８）??＜３ｃｍ（％）?１４（１１．６）?８（６．６）?２（１．７）?４（３．３）??ＵＩＣＣ?分期?０．００?ｌｃ??ＰＴ１（％）?１６（１３．２）
图３韦恩图??
【参考文献】：
期刊论文
[1]Metastasis-associated long noncoding RNAs in gastrointestinal cancer: Implications for novel biomarkers and therapeutic targets[J]. Fei-Fei Zhang,Yu-Hao Luo,Hui Wang,Liang Zhao. World Journal of Gastroenterology. 2016(39)
[2]Esophageal cancer: Risk factors,screening and endoscopic treatment in Western and Eastern countries[J]. María José Domper Arnal,ángel Ferrández Arenas,ángel Lanas Arbeloa. World Journal of Gastroenterology. 2015(26)
[3]Is inconsistency of α-fetoprotein level a good prognosticator for hepatocellular carcinoma recurrence?[J]. Chung-Bao Hsieh, Teng-Wei Chen, Division of General Sur-gery, Tri-Service General Hospital, Taipei 114, Taiwan, China Chung-Bao Hsieh, Kuo-Piao Chung, Graduate Institute of Health Care Organization Administration, National Taiwan University, Taipei 100, Taiwan, China Chi-Ming Chu, Section of Biostatistics and Informatics, De-partment of Epidemiology, School of Public Health, National Defense Medical Center, Taipei 114, Taiwan, ChinaHeng-Cheng Chu, Department of Internal Medicine, Tri-Service General Hospital, Taipei 114, Taiwan, ChinaCheng-Ping Yu, Department of Pathology, Tri-Service General Hospital, Taipei 114, Taiwan, China. World Journal of Gastroenterology. 2010(24)
本文编号：3267785
【文章来源】：郑州大学河南省 211工程院校
【文章页数】：65 页
【学位级别】：硕士
【部分图文】：
图１?Ｈ１９原位杂交??
?实验结果???相关，见图２。??＾?１?Ｃ３?水１５一年内死?Ｃ?？〇－，?１?＊＊：４??ｆＭ－?…?■■舰??＇：ＵＵ?＂？?Ｉｉ〇Ｉｊ?ｊ?ｊＩ??图２?ＥＳＣＣ样本中ＬｎｃＨｌ９?ＩＳＨ表达水平柱状图??注：Ａ：两个ＥＳＣＣ组中ＬｎｃＨｌ９ＩＳＨ表达水平差异＾?Ｂ：ＬｎｃＨｌ９表达水平与Ｕ１ＣＣ分期??的关系。＂＊?ｐ?＝?０．〇０ｌ。??表３?ＬｎｃＲＮＡ?ＨＩ９表达与食管鱗癌临床病理特点之间的关系??临床特征?总?弱阳性?中等阳性?强阳性?Ｐ值??（Ｎ＝ｌ２ｌ）?（Ｎ＝ｌ９）?ｆＮ＝６４）?（Ｎ＝３８）??生存分组?０．００丨ａ??术后一年内死亡（％）?６３（５２．１）?６（５．０）?１２（９．９）?４５（３７．２）??术后超过五年生存（％）?５８（４７．９）?２０（１６．５）?２０（１６．５）?１８（１４．９）??年龄?０．１３２ｂ??＞６０（％）?６０（４９．６）?１３（１０．７）?１５（１２．４）?３２（２６．４）??＜６０（％）?６１（５０．４）?１３（１０．７）?１７（１４．０）?３１（２５．６）??性别?０．５９１ｃ??女（％）?６３（５２．１）?１５（１２．４）?１８（１４．９）?３０（２４．８）??男（％）?５８（４７．９）?１?１（９．１）?１４（１１．６）?３３（２７．３）??肿瘤大小?０．００２ａ??＞３ｃｍ（％）?１０７（８８．４）?１８（１４．９）?３０（２４．８）?５９（４８．８）??＜３ｃｍ（％）?１４（１１．６）?８（６．６）?２（１．７）?４（３．３）??ＵＩＣＣ?分期?０．００?ｌｃ??ＰＴ１（％）?１６（１３．２）
图３韦恩图??
【参考文献】：
期刊论文
[1]Metastasis-associated long noncoding RNAs in gastrointestinal cancer: Implications for novel biomarkers and therapeutic targets[J]. Fei-Fei Zhang,Yu-Hao Luo,Hui Wang,Liang Zhao. World Journal of Gastroenterology. 2016(39)
[2]Esophageal cancer: Risk factors,screening and endoscopic treatment in Western and Eastern countries[J]. María José Domper Arnal,ángel Ferrández Arenas,ángel Lanas Arbeloa. World Journal of Gastroenterology. 2015(26)
[3]Is inconsistency of α-fetoprotein level a good prognosticator for hepatocellular carcinoma recurrence?[J]. Chung-Bao Hsieh, Teng-Wei Chen, Division of General Sur-gery, Tri-Service General Hospital, Taipei 114, Taiwan, China Chung-Bao Hsieh, Kuo-Piao Chung, Graduate Institute of Health Care Organization Administration, National Taiwan University, Taipei 100, Taiwan, China Chi-Ming Chu, Section of Biostatistics and Informatics, De-partment of Epidemiology, School of Public Health, National Defense Medical Center, Taipei 114, Taiwan, ChinaHeng-Cheng Chu, Department of Internal Medicine, Tri-Service General Hospital, Taipei 114, Taiwan, ChinaCheng-Ping Yu, Department of Pathology, Tri-Service General Hospital, Taipei 114, Taiwan, China. World Journal of Gastroenterology. 2010(24)
本文编号：3267785
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