Reduning Injection influenza A/H1N1 virus viral load lung in

  本文关键词：热毒宁注射液抗甲型H1N1流感病毒作用机制研究，由笔耕文化传播整理发布。

热毒宁注射液抗甲型H1N1流感病毒作用机制研究

Mechanism of Reduning Injection against influenza A/H1N1 virus

[1] [2] [3] [4] [5] [6]

WANG Zhen-zhong1＇ 2 BAO Lin-lin3, SUN Lan1＇ 2, BI Yu-an1＇ 2, ZHOU Jurl1＇ 2, XIAO Wei1＇ 2 1. Kanion Pharmaceutical Co., Ltd., Lianyungang 222001, China 2. State Key Laborato

[1]江苏康缘药业股份有限公司,江苏连云港222001; [2]中药制药过程新技术国家重点实验室,江苏连云港222001; [3]中国医学科学院医学实验动物研究所,北京100021

文章摘要：目的研究热毒宁注射液体内抗甲型H1N1流感病毒的作用及其机制。方法采用甲型H1N1流感病毒滴鼻感染BALB／c小鼠制备肺炎模型，，观察热毒宁注射液对甲型H1N1流感病毒感染小鼠平均存活时间、存活率、肺指数、病毒载量及细胞因子的影响。结果与模型组比较，热毒宁注射液显著提高病毒感染小鼠的存活率、延长小鼠平均存活时间（P〈0．05、0．01）；显著降低病毒感染小鼠肺指数、肺组织病毒载量（P〈0．05、0．01）；提高肺组织丫干扰素（IFN-γ）水平，降低白细胞介素-6（IL-6）、肿瘤坏死因子α（TNF-α）水平。结论热毒宁注射液对甲型H1N1流感病毒感染小鼠具有一定的保护作用，其作用机制可能与提高IFN-γ，水平，降低IL-6、TNF-α水平有关。

Abstr：Objective To investigate the effect of Reduning Injection （RI） on the resistance to influenzaA/H1N1 vires and its mechanism. Methods The pneumonic model of BALB/c mice was established by influenza A/H1N1 virus, and the average survival period, survival rate, lung index, viral load, and cytokine level of mice infected by influenza A/H1N1 virus were observed. Results Compared with the model group, RI significantly increased the survival rate and prolonged the average survival period of mice infected by influenza A/H1N1 virus （P 〈 0.05, 0.01）. RI significantly reduced the lung index, viral load, contents of IL-6 and TNF-α, while increased the content of IFN-γ （P 〈 0.05, 0.01）. Conclusion RI has the certain protective effect on mice infected by influenza A/H1N1 virus. Its mechanism may be related to the improvement of the content oflFN-y and the decrease of the contents of IL-6 and TNF-α.

文章关键词：

Keyword：:Reduning Injection influenza A/H1N1 virus viral load lung index cytokines

课题项目：现代中药创新集群与数字制药技术平台（2013ZX09402203）

  本文关键词：热毒宁注射液抗甲型H1N1流感病毒作用机制研究，由笔耕文化传播整理发布。